Anti-KCC2 Antibody (N1/66)

3 Citations

Our Anti-KCC2 mouse monoclonal primary antibody from NeuroMab is produced in-house from hybridoma clone N1/66. It detects chicken, human, mouse, and rat KCC2, and is purified by Protein A chromatography. It is great for use in IHC, ICC, WB.

Adult rat brain membrane immunoblot.
Adult rat brain membrane immunoblot.
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Adult rat brain membrane immunoblot.
Adult rat brain immunohistochemistry.

Chicken, Human, Mouse, Rat
ICC, IHC, WB
Mouse
490
550
594
650
Carrier-Free

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Data Sheet

SKU: 75-050

Volume: 100 µL
Price:
$329.00
Ships: 1-2 business days

Product Details

KCC2
Solute carrier family 12 member 5 is encoded by the gene SLC12A5 and belongs to the SLC12 family of cation-coupled chloride transporters. SLC12A5 is an integral membrane K-Cl cotransporter that mediates electroneutral potassium-chloride cotransport in mature neurons and is required for neuronal Cl(-) homeostasis. Diseases associated with SLC12A5 include Early Infantile Epileptic Encephalopathy 34 and Idiopathic Generalized Epilepsy 14.
Purified by Protein A chromatography
1 mg/mL
Monoclonal
N1/66
IgG1
ICC, IHC, WB
Mouse
Slc12a5 Kcc2 Kiaa1176
140 kDa
Fusion protein amino acids 932-1043 (intracellular carboxyl terminus) of rat KCC2b (accession number Q63633) produced recombinantly in E. Coli
Chicken, Human, Mouse, Rat
AB_2188800
Aliquot and store at ≤ -20°C for long term storage. For short term storage, store at 2-8°C. For maximum recovery of product, centrifuge the vial prior to removing the cap.
Liquid
Produced by in vitro bioreactor culture of hybridoma line followed by Protein A affinity chromatography. Purified mAbs are >90% specific antibody.
10 mM Tris, 50 mM Sodium Chloride, 0.065% Sodium Azide pH 7.4
ICC: 1:500
Unconjugated
Does not react with KCC1, KCC3 or KCC4
Each new lot of antibody is quality control tested by western blot on rat whole brain lysate and confirmed to stain the expected molecular weight band.
These antibodies are to be used as research laboratory reagents and are not for use as diagnostic or therapeutic reagents in humans.
United States
24 months from date of receipt
Shipped on ice packs
Solute carrier family 12 member 5 (Electroneutral potassium-chloride cotransporter 2) (K-Cl cotransporter 2) (mKCC2) (Neuronal K-Cl cotransporter)
Q63633

Product Specific References for Applications and Species

Immunocytochemistry: Human
PMID Dilution Publication
317961231:500Hinz, L., et al. 2019. KCC2 expression levels are reduced in post mortem brain tissue of Rett syndrome patients. Acta Neuropathology Communications, 196.
Immunocytochemistry: Chicken
PMID Dilution Publication
24722186not listedLaw, C., et al. 2014. Emergence of motor circuit activity. . PLoS One, e93836.
Western Blot: Human
PMID Dilution Publication
28496181not listedKursan, S., et al. 2017. The neuronal K+ Cl− co-transporter 2 (Slc12a5) modulates insulin secretion. . Scientific reports, .

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