Anti-KCC2 Antibody (N1/66)

Name: Anti-KCC2 Antibody
Brand: NeuroMab
SKU: 75-050
Price: 319.0 USD
Availability: InStock

Our Anti-KCC2 mouse monoclonal primary antibody from NeuroMab is produced in-house from hybridoma clone N1/66. It detects chicken, human, mouse, and rat KCC2, and is purified by Protein A chromatography. It is great for use in IHC, ICC, WB.

Adult rat brain membrane immunoblot.

Species Reactivity

Chicken, Human, Mouse, Rat

Applications

ICC, IHC, WB

Host Species

Mouse

Available Conjugates

490

550

594

650

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Data Sheet

SKU: 75-050

Volume: 100 µL

Variant

Price:

$319.00

Quantity:

Product Details

Target

KCC2

Target Description

Solute carrier family 12 member 5 is encoded by the gene SLC12A5 and belongs to the SLC12 family of cation-coupled chloride transporters. SLC12A5 is an integral membrane K-Cl cotransporter that mediates electroneutral potassium-chloride cotransport in mature neurons and is required for neuronal Cl(-) homeostasis. Diseases associated with SLC12A5 include Early Infantile Epileptic Encephalopathy 34 and Idiopathic Generalized Epilepsy 14.

Format

Purified by Protein A chromatography

Concentration

1 mg/mL

Clonality

Monoclonal

Clone

N1/66

Isotype

IgG1

Applications

ICC, IHC, WB

Host Species

Mouse

Gene Name

Slc12a5 Kcc2 Kiaa1176

Molecular Weight

140 kDa

Antigen

Fusion protein amino acids 932-1043 (intracellular carboxyl terminus) of rat KCC2b (accession number Q63633) produced recombinantly in E. Coli

Species Reactivity

Chicken, Human, Mouse, Rat

Antibody Registry ID

AB_2188800

Storage

Aliquot and store at ≤ -20°C for long term storage. For short term storage, store at 2-8°C. For maximum recovery of product, centrifuge the vial prior to removing the cap.

Physical State

Liquid

Production Notes

Produced by in vitro bioreactor culture of hybridoma line followed by Protein A affinity chromatography. Purified mAbs are >90% specific antibody.

Buffer

10 mM Tris, 50 mM Sodium Chloride, 0.065% Sodium Azide pH 7.4

Dilution Ranges

ICC: 1:500

Conjugate

Unconjugated

Specificity

Does not react with KCC1, KCC3 or KCC4

Quality Control

Each new lot of antibody is quality control tested by western blot on rat whole brain lysate and confirmed to stain the expected molecular weight band.

Usage Statement

These antibodies are to be used as research laboratory reagents and are not for use as diagnostic or therapeutic reagents in humans.

Country of Origin

United States

Expiration

24 months from date of receipt

Shipping

Shipped on ice packs

Synonyms

Solute carrier family 12 member 5 (Electroneutral potassium-chloride cotransporter 2) (K-Cl cotransporter 2) (mKCC2) (Neuronal K-Cl cotransporter)

UniProt Details

UniProt (Human): Q9H2X9
UniProt (Immunogen Species): Q63633

Product Specific References for Applications and Species

Immunocytochemistry: Human
Immunohistochemistry: Chicken
Western Blot: Human

Immunocytochemistry: Human
PMID	Dilution	Publication
31796123	1:500	Hinz, L., et al. 2019. KCC2 expression levels are reduced in post mortem brain tissue of Rett syndrome patients. Acta Neuropathology Communications, 196.

Immunocytochemistry: Chicken
PMID	Dilution	Publication
24722186	not listed	Law, C., et al. 2014. Emergence of motor circuit activity. . PLoS One, e93836.

Western Blot: Human
PMID	Dilution	Publication
28496181	not listed	Kursan, S., et al. 2017. The neuronal K+ Cl− co-transporter 2 (Slc12a5) modulates insulin secretion. . Scientific reports, .

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Anti-KCC2 Antibody (N1/66)

SKU: 75-050

Product Details

Product Specific References for Applications and Species

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