Anti-Kv2.2 Potassium Channel Antibody (N372B/60)

Name: Anti-Kv2.2 Potassium Channel Antibody
Brand: NeuroMab
SKU: 75-360
Price: 319.0 USD
Availability: InStock

9 Citations

Our Anti-Kv2.2 potassium channel mouse monoclonal primary antibody from NeuroMab is produced in-house from hybridoma clone N372B/60. It is KO validated, detects mouse and rat Kv2.2 potassium channel, and is purified by Protein A chromatography. It is great for use in IHC, ICC, WB.

Immunoblot against extracts of COS cells transiently transfected with GFP-tagged Kv2.2long, Myc-tagged Kv2.2short or untagged Kv2.1 plasmid probed with N372B/60 (left), N372C/51 (middle) or N52A/42 (right) TC supe.

Immunofluorescence staining of adult wild-type (WT) and Kv2.2 knockout (KO) mouse magnocellular preoptic nucleus (MCPO) with N372B/60 (red) and Hoechst nuclear stain (blue.

Species Reactivity

Mouse, Rat

Applications

ICC, IHC, WB

Host Species

Mouse

Available Conjugates

490

550

594

650

Go to Full Product Details

Data Sheet

KO Validated

SKU: 75-360

Volume: 100 µL

Variant

Price:

$319.00

Quantity:

Product Details

Target

Kv2.2 potassium channel

Target Description

Voltage-gated K+ channels are important determinants of neuronal membrane excitability (Pongs, 1999). Moreover, differences in K+ channel expression patterns and densities contribute to the variations in action potential waveforms and repetitive firing patterns evident in different neuronal cell types. The delayed rectifier-type (IK)channels (Kv1.5, Kv2.1, and Kv2.2) are expressed on all neuronal somata and proximal dendrites and are also found in a wide variety of non-neuronal cells types including pancreatic islets, alveolar cells and cardiac myocytes (Hwang et al., 1993; Yan et al., 2004; Michaelevski et al., 2003). Kv2.1 and Kv2.2 form distinct populations of K+ channels and these subunits are thought to be primarily responsible for IK in superior cervical ganglion cells (Blaine and Ribera, 1998; Burger and Ribera, 1996).

Format

Purified by Protein A chromatography

Concentration

1 mg/mL

Clonality

Monoclonal

Clone

N372B/60

Isotype

IgG2b

Applications

ICC, IHC, WB

Host Species

Mouse

Gene Name

Kcnb2

Molecular Weight

120 kDa

Antigen

Fusion protein amino acids 717-907 (cytoplasmic C-terminus) of rat Kv2.2 long isoform (accession number Q63099) produced recombinantly in E. Coli

Species Reactivity

Mouse, Rat

Antibody Registry ID

AB_2315868

Storage

Aliquot and store at ≤ -20°C for long term storage. For short term storage, store at 2-8°C. For maximum recovery of product, centrifuge the vial prior to removing the cap.

Physical State

Liquid

Production Notes

Produced by in vitro bioreactor culture of hybridoma line followed by Protein A affinity chromatography. Purified mAbs are >90% specific antibody.

Buffer

10 mM Tris, 50 mM Sodium Chloride, 0.065% Sodium Azide pH 7.125

Conjugate

Unconjugated

Specificity

Does not cross-react with Kv2.2 short isoformDoes not cross-react with Kv2.1

Quality Control

Each new lot of antibody is quality control tested by western blot on rat whole brain lysate and confirmed to stain the expected molecular weight band.

Usage Statement

These antibodies are to be used as research laboratory reagents and are not for use as diagnostic or therapeutic reagents in humans.

Country of Origin

United States

Expiration

24 months from date of receipt

Shipping

Shipped on ice packs

Synonyms

Potassium voltage-gated channel subfamily B member 2 (CDRK) (Voltage-gated potassium channel subunit Kv2.2)

UniProt Details

UniProt (Human): Q92953
UniProt (Immunogen Species): Q63099

Product Specific References for Applications and Species

Immunohistochemistry: Mouse | Rat
Western Blot: Mouse

Immunohistochemistry: Mouse
PMID	Dilution	Publication
38335058	1ug/ml	Stewart, RG, et al. 2024. Distinct cellular expression and subcellular localization of Kv2 voltage-gated K+ channel subtypes in dorsal root ganglion neurons conserved between mice and humans. The Journal of Comparative Neurology, e25575.
34435615	not listed	Newkirk, G.S., et al. 2022. Kv2.1 Potassium Channels Regulate Repetitive Burst Firing in Extratelencephalic Neocortical Pyramidal Neurons. Cerebral Cortex, 1055-1076.
33837049	1ug/ml	Irie, T., et al. 2021. Essential Role of Somatic Kv2 Channels in High-Frequency Firing in Cartwheel Cells of the Dorsal Cochlear Nucleus. eNeuro, ENEURO.0515-20.2021.
29483285	1:10 (supe)	Gayet-Primo, J., et al. 2018. Heteromeric KV2/KV8. 2 channels mediate delayed rectifier potassium currents in primate photoreceptors. Journal of Neuroscience, 3414-3427.
29403353	not listed	Bishop, H.I., et al. 2018. Kv2 Ion Channels Determine the Expression and Localization of the Associated AMIGO-1 Cell Adhesion Molecule in Adult Brain Neurons. Frontiers in Molecular Neuroscience, 1.
28856240	1:10 (supe)	Palacio, S., et al. 2017. Heterogeneity in Kv2 Channel Expression Shapes Action Potential Characteristics and Firing Patterns in CA1 versus CA2 Hippocampal Pyramidal Neurons. eNeuro, .
24494598	not listed	Speca, D.J., et al. 2014. Deletion of the Kv2.1 delayed rectifier potassium channel leads to neuronal and behavioral hyperexcitability.. Genes, Brain and Behavior, 394-408.

Immunohistochemistry: Rat
PMID	Dilution	Publication
30091655	10-20ug/ml	Kirmiz, M., et al. 2018. Remodeling neuronal ER-PM junctions is a conserved nonconducting function of Kv2 plasma membrane ion channels. MolecularBiology of the Cell, 2410-2432.

Western Blot: Mouse
PMID	Dilution	Publication
26538660	1:5 (supe)	Bishop, H.I., et al. 2015. Distinct Cell- and Layer-Specific Expression Patterns and Independent Regulation of Kv2 Channel Subtypes in Cortical Pyramidal Neurons.. J Neurosci, 14922-14942.

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Anti-Kv2.2 Potassium Channel Antibody (N372B/60)

SKU: 75-360

Product Details

Product Specific References for Applications and Species

Bulk Order Anti-Kv2.2 Potassium Channel Antibody

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