Anti-Kv3.3 Potassium Channel Antibody (N375/67)

Name: Anti-Kv3.3 Potassium Channel Antibody
Brand: NeuroMab
SKU: 75-354
Price: 319.0 USD
Availability: InStock

3 Citations

Our Anti-Kv3.3 potassium channel mouse monoclonal primary antibody from NeuroMab is produced in-house from hybridoma clone N375/67. It detects human, mouse, and rat Kv3.3 potassium channel, and is purified by Protein A chromatography. It is great for use in IHC, ICC, WB.

Adult rat hippocampus immunohistochemistry.

$Immunoblot against adult rat brain membrane (RBM) fractions and extracts of COS cells transiently transfected with untagged Kv3.3a, Kv3.3b or Kv2.1 plasmid probed with N375/67 (left) or N52A/42 (right) TC supe.$

Adult rat hippocampus immunohistochemistry.

Species Reactivity

Human, Mouse, Rat

Applications

ICC, IHC, WB

Host Species

Mouse

Available Conjugates

490

550

594

650

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Data Sheet

SKU: 75-354

Volume: 100 µL

Variant

Price:

$319.00

Quantity:

Product Details

Target

Kv3.3 potassium channel

Target Description

Potassium voltage-gated channel subfamily C member 3 or Kv3.3 potassium channel, is a member of the potassium channel, voltage-gated, shaker-related subfamily and the Shaw subfamily (the family includes 4 members Kv3.1-Kv3.4). Note that this protein has several splice variants and that antibody N375/67 recognizes both Kv3.3b and Kv3.3a. Kv3.3 is primarily expressed in brain. Kv3.3 is highly expressed in purkinje cells and deep cerebellar nuclei in the cerebellum. Kv3.3a is also expressed in brain, but not found in the cerebellum. Kv3.3 is a membrane protein that mediates the voltage-dependent potassium ion permeability of excitable membranes. Diseases associated with the gene for this protein (Kcnc3) include Spinocerebellar Ataxia 13 and Autosomal recessive ataxic cerebral palsy.

Format

Purified by Protein A chromatography

Concentration

1 mg/mL

Clonality

Monoclonal

Clone

N375/67

Isotype

IgG1

Applications

ICC, IHC, WB

Host Species

Mouse

Gene Name

Kcnc3

Molecular Weight

80-100 kDa (depending on isoform)

Antigen

Fusion protein amino acids 628-728 (cytoplasmic C-terminus) of mouse Kv3.3b (accession number Q63959) produced recombinantly in E. Coli

Species Reactivity

Human, Mouse, Rat

Antibody Registry ID

AB_2315872

Storage

Aliquot and store at ≤ -20°C for long term storage. For short term storage, store at 2-8°C. For maximum recovery of product, centrifuge the vial prior to removing the cap.

Physical State

Liquid

Production Notes

Produced by in vitro bioreactor culture of hybridoma line followed by Protein A affinity chromatography. Purified mAbs are >90% specific antibody.

Buffer

10 mM Tris, 50 mM Sodium Chloride, 0.065% Sodium Azide pH 7.125

Conjugate

Unconjugated

Specificity

Does not cross-react with Kv3.4 or Kv3.2

Quality Control

Each new lot of antibody is quality control tested by western blot on rat whole brain lysate and confirmed to stain the expected molecular weight band.

Usage Statement

These antibodies are to be used as research laboratory reagents and are not for use as diagnostic or therapeutic reagents in humans.

Country of Origin

United States

Expiration

24 months from date of receipt

Shipping

Shipped on ice packs

Synonyms

Potassium voltage-gated channel subfamily C member 3 (KSHIIID) (Voltage-gated potassium channel subunit Kv3.3)

UniProt Details

UniProt (Human): O14649
UniProt (Immunogen Species): Q63959

Product Specific References for Applications and Species

Immunocytochemistry: Rat
Immunohistochemistry: Mouse

Immunocytochemistry: Rat
PMID	Dilution	Publication
33154197	1:50	Kim, W.B., et al. 2020. Distribution of K v 3 Subunits in Cochlear Afferent and Efferent Nerve Fibers Implies Distinct Role in Auditory Processing. Experimental Neurobiology, 344-355.

Immunohistochemistry: Mouse
PMID	Dilution	Publication
35510987	1:3000	Richardson, A., et al. 2022. Kv3.3 subunits control presynaptic action potential waveform and neurotransmitter release at a central excitatory synapse. Elife, e75219.
31201232	1:300	Hosoi, N., et al. 2019. Deletion of Class II ADP-Ribosylation Factors in Mice Causes Tremor by the Nav1.6 Loss in Cerebellar Purkinje Cell Axon Initial Segments. Journal of Neuroscience, 6339-6353.

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Anti-Kv3.3 Potassium Channel Antibody (N375/67)

SKU: 75-354

Product Details

Product Specific References for Applications and Species

Bulk Order Anti-Kv3.3 Potassium Channel Antibody

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