Our Anti-Kv3.4 K+ channel mouse monoclonal primary antibody from NeuroMab is produced in-house from hybridoma clone N72/16. It detects human, mouse, and rat Kv3.4 K+ channel, and is purified by Protein A chromatography. It is great for use in IHC, ICC, IP, WB.
Immunoblot against crude C. elegans worm extracts and brain membranes from adult rat (RBM) probed with N72/16 TC supe.
Potassium voltage-gated channel subfamily C member 4 or Kv3.4 potassium channel, is a member of the potassium channel, voltage-gated, shaker-related subfamily and the Shaw subfamily (the family includes 4 members Kv3.1-Kv3.4). Kv3.4 is primarily expressed in brain and found in the cerebellum, hippocampus, brain stem and spinal cord. It has also been found in skeletal and arterial smooth muscle. Kv3.4 is a membrane protein that mediates the voltage-dependent potassium ion permeability of excitable membranes. Diseases associated with the gene for this protein (Kcnc4) include Spinocerebellar Ataxia 13 and Autosomal recessive ataxic cerebral palsy. Kv3.4 is also over-expressed in the early stages of Alzheimer’s disease and has been implicated in chronic hypoxia and Parkinson’s disease.
Purified by Protein A chromatography
1 mg/mL
Monoclonal
N72/16
IgG1
ICC, IHC, IP, WB
Mouse
Kcnc4
70 kDa (100 kDa in brain due to glycosylation)
Synthetic peptide amino acids 175-192 (GDEAGDDERELALQRLGP) of rat Kv3.4 (accession number Q63734)
Human, Mouse, Rat
AB_10673277
Aliquot and store at ≤ -20°C for long term storage. For short term storage, store at 2-8°C. For maximum recovery of product, centrifuge the vial prior to removing the cap.
Liquid
Produced by in vitro bioreactor culture of hybridoma line followed by Protein A affinity chromatography. Purified mAbs are >90% specific antibody.
10 mM Tris, 50 mM Sodium Chloride, 0.065% Sodium Azide pH 7.40
WB: 1: 1000
IHC: 1:500
ICC: 1:500
IP: 50 ug
Unconjugated
No cross-reactivity reported
Each new lot of antibody is quality control tested by western blot on rat whole brain lysate and confirmed to stain the expected molecular weight band.
These antibodies are to be used as research laboratory reagents and are not for use as diagnostic or therapeutic reagents in humans.
United States
24 months from date of receipt
Shipped on ice packs
Potassium voltage-gated channel subfamily C member 4 (Raw3) (Voltage-gated potassium channel subunit Kv3.4)
Kim, W.B., et al. 2020. Distribution of K v 3 Subunits in Cochlear Afferent and Efferent Nerve Fibers Implies Distinct Role in Auditory Processing. Experimental Neurobiology, 344-355.
Hartmann, S., et al. 2018. β-Secretase BACE1 Promotes Surface Expression and Function of Kv3.4 at Hippocampal Mossy Fiber Synapses. Journal of Neuroscience, 3480-3494.
Gross, C., et al. 2011. Fragile X mental retardation protein regulates protein expression and mRNA translation of the potassium channel Kv4.2.. Journal of Neuroscience, 5693-5698.
Gittis, A.H., et al. 2010. Mechanisms of sustained high firing rates in two classes of vestibular nucleus neurons: differential contributions of resurgent Na, Kv3, and BK currents.. Journal of Neurophysiology, 1625-1635.
DiFranco, M., et al. 2012. The delayed rectifier potassium conductance in the sarcolemma and the transverse tubular system membranes of mammalian skeletal muscle fibers.. The Journal of General Physiology, 109-137.
Pacheco Otalora, L.F., et al. 2011. Chronic deficit in the expression of voltage-gated potassium channel Kv3.4 subunit in the hippocampus of pilocarpine-treated epileptic rats.. Brain Research, 308-316.
DiFranco, M., et al. 2012. The delayed rectifier potassium conductance in the sarcolemma and the transverse tubular system membranes of mammalian skeletal muscle fibers.. The Journal of General Physiology, 109-137.
Sun, W., et al. 2011. DPP6 establishes the A-type K(+) current gradient critical for the regulation of dendritic excitability in CA1 hippocampal neurons.. Neuron, 1102-1115.
Sun W, et al. 2011. DPP6 establishes the A-type K(+) current gradient critical for the regulation of dendritic excitability in CA1 hippocampal neurons.. Neuron, 1102-15.
Dufour, M.A., et al. 2014. Somatodendritic ion channel expression in substantia nigra pars compacta dopaminergic neurons across postnatal development.. Journal of Neuroscience Research, 981-999.
