Anti-Kv4.3 K+ Channel Antibody (K75/41)
Our Anti-Kv4.3 K+ channel mouse monoclonal primary antibody from NeuroMab is produced in-house from hybridoma clone K75/41. It is KO validated, detects drosophila, guinea pig, human, mouse, and rat Kv4.3 K+ channel, and is purified by Protein A chromatography. It is great for use in IHC, ICC, IP, WB.
![Immunoblots on brain membranes prepared from whole rat (RBM) and mouse (MBM) brain, and from human cerebral cortex [HBM(Cx)], and hippocampus [HBM(H)].](http://www.antibodiesinc.com/cdn/shop/files/73-017-k75-41-wb-2_{width}x.jpg?v=1703185935)
Electron micrograph shows K75/41 immunolabelling in the hippocampus using a pre-embedding immunogold method. Immunogold particles (arrows) were observed along the extrasynaptic plasma membrane of dendritic shafts (Den) of interneurons establishing synaptic contacts with excitatory axon terminals (b). Scale bar = 500 nm. Courtesy Dr. Rafael Lujan (Universidad de Castilla-La Mancha).
![Immunoblots on brain membranes prepared from whole rat (RBM) and mouse (MBM) brain, and from human cerebral cortex [HBM(Cx)], and hippocampus [HBM(H)].](http://www.antibodiesinc.com/cdn/shop/files/73-017-k75-41-wb-2_1024x.jpg?v=1703185935)
Drosophila, Hamster, Human, Mouse, Rat
ICC, IHC, IP, WB
Mouse
KO Validated
Product Specific References for Applications and Species
- Array Tomography: Rat
- HIstoblotting: Mouse
- Immunocytochemistry: Human | Mouse | Rat
- Immunohistochemistry: Mouse | Rat
- Immunoprecipitation: Human | Mouse
- Western Blot: Drosophila | Guinea Pig | Human | Mouse | Non-Human Primate | Rabbit| Rat
- Additional Publications: Unspecified
| Array Tomography: Rat | ||
| PMID | Dilution | Publication |
| 26400949 | 1:600 | Simkin, D., et al. 2015. Aging-related hyperexcitability in CA3 pyramidal neurons is mediated by enhanced A-type K+ channel function and expression. . Journal of Neuroscience, 13206-13218. |
| Histoblotting: Mouse | ||
| PMID | Dilution | Publication |
| 30634540 | not listed | Alfaro-Ruíz, R., et al. 2019. Expression, cellular and subcellular localisation of Kv4. 2 and Kv4. 3 channels in the rodent hippocampus. . International journal of molecular sciences, 246. |
| Immunocytochemistry: Human | ||
| PMID | Dilution | Publication |
| 36097791 | 1:400 | Chiu, C.Y., et al. 2022. K+ Channel Kv4.1 is Expressed in the Nociceptors/Secondary Nociceptive Neurons and Participates in Pain Regulation. European Journal of Pain, 2238-2256. |
| 30588629 | not listed | Tyan, L., et al. 2019. Long QT syndrome caveolin‐3 mutations differentially modulate Kv4 and Cav1. 2 channels to contribute to action potential prolongation. . The Journal of physiology, 1531-1551. |
| 23324056 | 1:5 (supe) | David, J.P., et al. 2013. Trafficking of the IKs -complex in MDCK cells: site of subunit assembly and determinants of polarized localization.. Traffic, 399-411. |
| Immunocytochemistry: Mouse | ||
| PMID | Dilution | Publication |
| 31064843 | not listed | Lubbers, E.R., et al. 2019. Defining new mechanistic roles for αii spectrin in cardiac function. . Journal of Biological Chemistry, 9576-9591. |
| 29802197 | 1:10 (supe) | Cheng, L., et al. 2018. Coupling of Smoothened to inhibitory G proteins reduces voltage-gated K+ currents in cardiomyocytes and prolongs cardiac action potential duration. . Journal of Biological Chemistry, 11022-11032. |
| 29802197 | 1:10 (supe) | Cheng, L., et al. 2018. Coupling of Smoothened to inhibitory G proteins reduces voltage-gated K+ currents in cardiomyocytes and prolongs cardiac action potential duration. . Journal of Biological Chemistry, 11022-11032. |
| 26196737 | 1:100 | Liu, J., et al. 2015. Kv4.3-Encoded Fast Transient Outward Current Is Presented in Kv4.2 Knockout Mouse Cardiomyocytes.. PLoS One, e0133274. |
| 21507952 | 1:10 (supe) | Cheng, L., et al. 2011. Cortactin is required for N-cadherin regulation of Kv1.5 channel function.. The Journal of Biological Chemistry, 20478-20489. |
| Immunocytochemistry: Rat | ||
| PMID | Dilution | Publication |
| 26239200 | 1:8000 | Cheng, C.F., et al. 2016. Coexpression of auxiliary subunits KChIP and DPPL in potassium channel Kv4-positive nociceptors and pain-modulating spinal interneurons.. Journal of Comparative Neurology, 846-73. |
| 26016905 | 1:200 | You, T., et al. 2015. Two novel Brugada syndrome-associated mutations increase KV4.3 membrane expression and function.. International Journal of Molecular Medicine, 309-315. |
| Immunohistochemistry: Mouse | ||
| PMID | Dilution | Publication |
| 38942263 | 1:1000 | Esaki, H, et al. 2024. Role of medial prefrontal cortex voltage-dependent potassium 4.3 channels in nicotine-induced enhancement of object recognition memory in male mice. European journal of pharmacology, 176790. |
| 36640913 | 1:500 | Otuyemi, B., et al. 2023. Domain and Cell Type-specific Immunolocalisation of Voltage-gated Potassium Channels in the Mouse Striatum. Journal of Chemical Neuroanatomy, 102233. |
| 36097791 | 1:400 | Chiu, C.Y., et al. 2022. K+ Channel Kv4.1 is Expressed in the Nociceptors/Secondary Nociceptive Neurons and Participates in Pain Regulation. European Journal of Pain, 2238-2256. |
| 35098924 | 1:1500 | Hobson, B.D., et al. 2022. Subcellular proteomics of dopamine neurons in the mouse brain. eLife, e70921. |
| 31248728 | 1:250 | Zheng, Y., et al. 2019. Deep sequencing of somatosensory neurons reveals molecular determinants of intrinsic physiological properties. . Neuron, 598-616. |
| 31248728 | 1:250 | Zheng, Y., et al. 2019. Deep sequencing of somatosensory neurons reveals molecular determinants of intrinsic physiological properties. . Neuron, 598-616. |
| 30634540 | not listed | Alfaro-Ruíz, R., et al. 2019. Expression, cellular and subcellular localisation of Kv4. 2 and Kv4. 3 channels in the rodent hippocampus. . International journal of molecular sciences, 246. |
| 22612819 | not listed | Menegola, M., et al. 2012. The importance of immunohistochemical analyses in evaluating the phenotype of Kv channel knockout mice.. Epilepsia, 142-149. |
| 21676499 | 0.25 | Wykes, R., et al. 2012. Changes in the physiology of CA1 hippocampal pyramidal neurons in preplaque CRND8 mice. . Neurobiology of aging, 1609-1623. |
| 21329741 | 1:500 | Prüss, H., et al. 2011. Potassium channel expression in adult murine neural progenitor cells.. Neuroscience, 19-29. |
| 17122053 | 1:1000 | Burkhalter, A., et al. 2006. Differential expression of I(A) channel subunits Kv4.2 and Kv4.3 in mouse visual cortical neurons and synapses.. Journal of Neuroscience, 12274-82. |
| 17122038 | not listed | Menegola, M., et al. 2006. Unanticipated region- and cell-specific downregulation of individual KChIP auxiliary subunit isotypes in Kv4.2 knock-out mouse brain.. Journal of Neuroscience, 2137-42. |
| Immunohistochemistry: Rat | ||
| PMID | Dilution | Publication |
| 36181963 | 1:400 | Chiu, C.Y., et al. 2022. NS5806 Inhibits ERK Activation to Attenuate Pain Induced by Peripheral Nerve Injury. Neuroscience Letters, 136890. |
| 32899153 | not listed | Alfaro-Ruíz, R., et al. 2020. Cellular and subcellular localisation of kv4-associated kchip proteins in the rat cerebellum. . International journal of molecular sciences, 6403. |
| 32726631 | not listed | Holderith, N., et al. 2020. A High-Resolution Method for Quantitative Molecular Analysis of Functionally Characterized Individual Synapses. Cell Reports, 107968. |
| 32490811 | 1:500 | Oláh, V.J., et al. 2020. Functional specification of CCK+ interneurons by alternative isoforms of Kv4. 3 auxiliary subunits. . Elife, e58515. |
| 28330877 | 1:4000 | Kuo, Y., et al. 2017. K + Channel Modulatory Subunits KChIP and DPP Participate in Kv4-Mediated Mechanical Pain Control. Journal of Neuroscience, 4391-4404. |
| 27807163 | 1:500 | Rizwan, A.P., et al. 2016. Long-Term potentiation at the mossy fiber–granule cell relay invokes postsynaptic second-messenger regulation of Kv4 channels.. Journal of Neuroscience, 11196-11207. |
| 26239200 | 1:4000 | Cheng, C.F., et al. 2016. Coexpression of auxiliary subunits KChIP and DPPL in potassium channel Kv4-positive nociceptors and pain-modulating spinal interneurons.. Journal of Comparative Neurology, 846-73. |
| 26400949 | 1:500 | Simkin, D., et al. 2015. Aging-related hyperexcitability in CA3 pyramidal neurons is mediated by enhanced A-type K+ channel function and expression. . Journal of Neuroscience, 13206-13218. |
| 25355692 | 1:100-1:1000 | Wang, W.C., et al. 2015. Immunohistochemical localization of DPP10 in rat brain supports the existence of a Kv4/KChIP/DPPL ternary complex in neurons.. Journal of Comparative Neurology, 608-628. |
| 24037673 | not listed | Rainnie, D.G., et al. 2014. Distribution and functional expression of Kv4 family α subunits and associated KChIP β subunits in the bed nucleus of the stria terminalis.. Journal of Comparative Neurology, 609-25. |
| 22820170 | 1:200 | Matsuyoshi, H., et al. 2012. Distinct cellular distributions of Kv4 pore-forming and auxiliary subunits in rat dorsal root ganglion neurons.. Life Sciences, 258-263. |
| 22473424 | 1:100 | Huang, C.Y., et al. 2012. Coexpression of high-voltage-activated ion channels Kv3.4 and Cav1.2 in pioneer axons during pathfinding in the developing rat forebrain.. Journal of Comparative Neurology, 3650-3672. |
| 21451062 | not listed | Guan, D., et al. 2011. Postnatal development of A-type and Kv1- and Kv2-mediated potassium channel currents in neocortical pyramidal neurons.. Journal of Neurophysiology, 2976-88. |
| 21129448 | 1:1000 | Bourdeau, M.L., et al. 2011. KChIP1 modulation of Kv4.3-mediated A-type K(+) currents and repetitive firing in hippocampal interneurons.. Neuroscience, 173-87. |
| 20849929 | 1:1000 | Dabrowska, J., et al. 2010. Expression and distribution of Kv4 potassium channel subunits and potassium channel interacting proteins in subpopulations of interneurons in the basolateral amygdala.. Neuroscience, 721-33. |
| 18727953 | not listed | Monaghan, M.M., et al. 2008. Altered expression and localization of hippocampal A-type potassium channel subunits in the pilocarpine-induced model of temporal lobe epilepsy.. Neuroscience, 550-562. |
| 18495361 | 1:500 | Menegola, M., et al. 2008. Dendritic A-type potassium channel subunit expression in CA1 hippocampal interneurons.. Neuroscience, 953-64. |
| 18495361 | not listed | Menegola, M., et al. 2008. Dendritic A-type potassium channel subunit expression in CA1 hippocampal interneurons.. Neuroscience, 953-64. |
| 16525047 | 1:500 | Kollo, M., et al. 2006. Novel subcellular distribution pattern of A-type K+ channels on neuronal surface. Journal of Neuroscience, 2684-91. |
| Immunoprecipitation: Human | ||
| PMID | Dilution | Publication |
| 22198508 | 0.8 ug | Zhang, Y.H., et al. 2012. Modulation of human cardiac transient outward potassium current by EGFR tyrosine kinase and Src-family kinases.. Cardiovascular Research, 424-433. |
| Immunoprecipitation: Mouse | ||
| PMID | Dilution | Publication |
| 38891076 | 12ug | Belghazi, M, et al. 2024. High-Resolution Proteomics Unravel a Native Functional Complex of Cav1.3, SK3, and Hyperpolarization-Activated Cyclic Nucleotide-Gated Channels in Midbrain Dopaminergic Neurons. Cells, . |
| 31064843 | 2ug | Lubbers, E.R., et al. 2019. Defining new mechanistic roles for αii spectrin in cardiac function. . Journal of Biological Chemistry, 9576-9591. |
| Western Blot: Drosophila | ||
| PMID | Dilution | Publication |
| 34932577 | 1:500 | Vallejos, M.J., et al. 2021. Age-related changes in Kv4/Shal and Kv1/Shaker expression in Drosophila and a role for reactive oxygen species. PloS One, e0261087. |
| Western Blot: Guinea Pig | ||
| PMID | Dilution | Publication |
| 26764482 | 1:500 | Nassal, D.M., et al. 2016. Myocardial KChIP2 expression in guinea pig resolves an expanded electrophysiologic role. . PloS one, e0146561. |
| Western Blot: Human | ||
| PMID | Dilution | Publication |
| 31074016 | 1:1000 | Wang, Y., et al. 2019. Contribution of potassium channels to action potential repolarization of human embryonic stem cell‐derived cardiomyocytes. . British Journal of Pharmacology, 2780-2794. |
| 25854634 | 1:1000 | Duarri, A., et al. 2015. Spinocerebellar ataxia type 19/22 mutations alter heterocomplex Kv4.3 channel function and gating in a dominant manner.. Cell Mol Life Sciences, 3387-3399. |
| 22160437 | not listed | Marshall, G.E., et al. 2011. Remodelling of human atrial K+ currents but not ion channel expression by chronic β-blockade.. Pflügers Archiv: European Journal of Physiology, 537-548. |
| Western Blot: Mouse | ||
| PMID | Dilution | Publication |
| 36842733 | not listed | Markandeya, Y.S., et al. 2023. Caveolin-3 and Caveolae Regulate Ventricular Repolarization. Journal of Molecular and Cellular Cardiology, 38-49. |
| 32822706 | not listed | Tiwari, D., et al. 2020. The potassium channel Kv4. 2 regulates dendritic spine morphology, electroencephalographic characteristics and seizure susceptibility in mice.. Experimental Neurology, 113437. |
| 31064843 | not listed | Lubbers, E.R., et al. 2019. Defining new mechanistic roles for αii spectrin in cardiac function. . Journal of Biological Chemistry, 9576-9591. |
| 27023865 | not listed | Schilling, J.M., et al. 2016. Electrophysiology and metabolism of caveolin-3-overexpressing mice. . Basic research in cardiology, 1-13. |
| 27023865 | not listed | Schilling, J.M., et al. 2016. Electrophysiology and metabolism of caveolin-3-overexpressing mice. . Basic research in cardiology, 1-13. |
| 26611208 | not listed | Zhang, X., et al. 2016. Persistent increases in Ca(2+) influx through Cav1.2 shortens action potential and causes Ca(2+) overload-induced afterdepolarizations and arrhythmias.. Basic Research in Cardiology, 1-13. |
| 24375641 | 1:200 | Lopez-Izquierdo, A., et al. 2014. The absence of insulin signaling in the heart induces changes in potassium channel expression and ventricular repolarization.. American Journal of Physiology-Heart and Circulatory Physiology, H747-H754. |
| 21943606 | 1:1000 | Sun, W., et al. 2011. DPP6 establishes the A-type K(+) current gradient critical for the regulation of dendritic excitability in CA1 hippocampal neurons.. Neuron, 1102-15. |
| 21646717 | not listed | Stein, A.B., et al. 2011. Loss of H3K4 methylation destabilizes gene expression patterns and physiological functions in adult murine cardiomyocytes.. Journal of Clinical Investigation, 2641-2650. |
| 21507952 | not listed | Cheng, L., et al. 2011. Cortactin is required for N-cadherin regulation of Kv1.5 channel function.. The Journal of Biological Chemistry, 20478-20489. |
| 20943905 | not listed | Norris, A.J., et al. 2010. Interdependent roles for accessory KChIP2, KChIP3, and KChIP4 subunits in the generation of Kv4-encoded IA channels in cortical pyramidal neurons.. Journal of Neuroscience, 13644-13655. |
| 18045613 | not listed | Niwa, N., et al. 2008. Kv4.3 is not required for the generation of functional Ito,f channels in adult mouse ventricles.. Journal of Molecular and Cellular Cardiology, 95-104. |
| Western Blot: Rabbit | ||
| PMID | Dilution | Publication |
| 22160437 | not listed | Marshall, G.E., et al. 2011. Remodelling of human atrial K+ currents but not ion channel expression by chronic β-blockade.. Pflügers Archiv: European Journal of Physiology, 537-548. |
| Western Blot: Rat | ||
| PMID | Dilution | Publication |
| 29881800 | not listed | Lai, Y.C., et al. 2018. Myocardial remodeling and susceptibility to ventricular tachycardia in a model of chronic epilepsy. . Epilepsia open, 213-223. |
| 28677679 | 1:100 | Sakai, A., et al. 2017. MicroRNA cluster miR-17-92 regulates multiple functionally related voltage-gated potassium channels in chronic neuropathic pain. . Nature communications, 1-13. |
| 28263709 | 1:500 | Nassal, D.M., et al. 2017. KChIP2 is a core transcriptional regulator of cardiac excitability.. Elife, 17304. |
| 27259067 | 1:1000 | Lei, Z., et al. 2016. Reduced expression of IA channels is associated with post-ischemic seizures.. Epilepsy Research, 40-48. |
| 26400949 | 1 : 1000 | Simkin, D., et al. 2015. Aging-related hyperexcitability in CA3 pyramidal neurons is mediated by enhanced A-type K+ channel function and expression. . Journal of Neuroscience, 13206-13218. |
| 23066017 | 1:2000 | Yao, J.J., et al. 2012. Neuritin activates insulin receptor pathway to up-regulate Kv4.2-mediated transient outward K+ current in rat cerebellar granule neurons.. The Journal of Biological Chemistry, 41534-41545. |
| 23066017 | 1:2000 | Yao, J.J., et al. 2012. Neuritin activates insulin receptor pathway to up-regulate Kv4.2-mediated transient outward K+ current in rat cerebellar granule neurons.. The Journal of Biological Chemistry, 41534-41545. |
| 22180649 | not listed | Zhang, M., et al. 2012. KCNE2 protein is more abundant in ventricles than in atria and can accelerate hERG protein degradation in a phosphorylation-dependent manner.. American Journal of Physiology-Heart and Circulatory Physiology, H910-H922. |
| 22160437 | not listed | Marshall, G.E., et al. 2011. Remodelling of human atrial K+ currents but not ion channel expression by chronic β-blockade.. Pflügers Archiv: European Journal of Physiology, 537-548. |
| 20849929 | 1:1000 | Dabrowska, J., et al. 2010. Expression and distribution of Kv4 potassium channel subunits and potassium channel interacting proteins in subpopulations of interneurons in the basolateral amygdala.. Neuroscience, 721-33. |
| 19372218 | not listed | Jiang, M., et al. 2009. Dynamic partnership between KCNQ1 and KCNE1 and influence on cardiac IKs current amplitude by KCNE2.. The Journal of Biological Chemistry, 16452-16462. |
| 18458082 | not listed | Schwenk, J., et al. 2008. NMR analysis of KChIP4a reveals structural basis for control of surface expression of Kv4 channel complexes.. The Journal of Biological Chemistry, 18937-18946. |
| Additional Publications: Unspecified | ||
| PMID | Publication | |
| 26121254 | Cheng, C.F., et al. 2015. Nerve growth factor-induced synapse-like structures in contralateral sensory ganglia contribute to chronic mirror-image pain.. Pain, 2295-2309. | |
| 25447080 | Gillet, L., et al. 2015. Cardiac-specific ablation of synapse-associated protein SAP97 in mice decreases potassium currents but not sodium current. Heart Rhythm, 181-192. | |
| 25043828 | Lei, Z., et al. 2014. Reduced expression of IA channels is associated with postischemic seizures in hyperglycemic rats.. Journal of Neuroscience Research, 1775-1784. | |
| 24723263 | Dufour, M.A., et al. 2014. Somatodendritic ion channel expression in substantia nigra pars compacta dopaminergic neurons across postnatal development.. Journal of Neuroscience Research, 981-999. | |
| 23963749 | Duarri, A., et al. 2013. The L450F [Corrected] mutation in KCND3 brings spinocerebellar ataxia and Brugada syndrome closer together.. NeuroGenetics, 257-258. | |
| 23280838 | Duarri, A., et al. 2012. Mutations in potassium channel kcnd3 cause spinocerebellar ataxia type 19.. Annals of Neurology, 870-880. | |
| 22155597 | Hu, D., et al. 2012. A novel rare variant in SCN1Bb linked to Brugada syndrome and SIDS by combined modulation of Nav1. 5 and Kv4. 3 channel currents. . Heart Rhythm, 760-769. | |
| 21895522 | Lei, Z., et al. 2012. Alterations of A-type potassium channels in hippocampal neurons after traumatic brain injury.. Journal of Neurotrauma, 235-245. | |
| 22220205 | Sethi, J., et al. 2011. Kv4.2 mediates histamine modulation of preoptic neuron activity and body temperature.. PLoS One, e29134. | |
| 21849540 | Hourez, R., et al. 2011. Aminopyridines correct early dysfunction and delay neurodegeneration in a mouse model of spinocerebellar ataxia type 1.. Journal of Neuroscience, 11795-11807. | |
| 21507952 | Cheng, L., et al. 2011. Cortactin is required for N-cadherin regulation of Kv1.5 channel function.. The Journal of Biological Chemistry, 20478-20489. | |
| 21440618 | Nowak, A., et al. 2011. Kv3.1b and Kv3.3 channel subunit expression in murine spinal dorsal horn GABAergic interneurones.. Journal of Chemical Neuroanatomy, 30-38. | |
| 21115483 | Schwetz, T.A., et al. 2011. Sialic acids attached to O-glycans modulate voltage-gated potassium channel gating.. The Journal of Biological Chemistry, 4123-4132. | |
| 20650612 | Bealer, S.L., et al. 2010. Autonomic and cellular mechanisms mediating detrimental cardiac effects of status epilepticus.. Epilepsy Research, 66-73. | |
| 20371829 | Norris, A.J., et al. 2010. Molecular dissection of I(A) in cortical pyramidal neurons reveals three distinct components encoded by Kv4.2, Kv4.3, and Kv1.4 alpha-subunits.. Journal of Neuroscience, 5092-5101. | |
| 20354865 | Cotella, D., et al. 2010. Impaired glycosylation blocks DPP10 cell surface expression and alters the electrophysiology of Ito channel complex.. Pflügers Archiv: European Journal of Physiology, 87-97. | |
| 19668710 | Phuket, T.R., et al. 2009. Kv4 Channels Underlie the Subthreshold-Operating A-type K-current in Nociceptive Dorsal Root Ganglion Neurons.. Frontiers in Molecular Neuroscience, 3. | |
| 19344330 | Eyre, M.D., et al. 2009. Molecular diversity of deep short-axon cells of the rat main olfactory bulb.. European Journal of Neuroscience, 1397-1407. | |
| 18978958 | Clark, B.D., et al. 2008. DPP6 Localization in Brain Supports Function as a Kv4 Channel Associated Protein.. Frontiers in Molecular Neuroscience, 8. | |
| 18276729 | Amarillo, Y., et al. 2008. Ternary Kv4.2 channels recapitulate voltage-dependent inactivation kinetics of A-type K+ channels in cerebellar granule neurons.. The Journal of Physiology, 2093-2106. | |
| 17855600 | Chien, L.Y., et al. 2007. Reduced expression of A-type potassium channels in primary sensory neurons induces mechanical hypersensitivity.. Journal of Neuroscience, 9855-9865. | |
| 17314290 | Bourdeau, M.L., et al. 2007. Kv4.3-mediated A-type K+ currents underlie rhythmic activity in hippocampal interneurons.. Journal of Neuroscience, 1942-1953. | |