Anti-LRP4 (Extracellular) Antibody (N207/27)

Name: Anti-LRP4 (Extracellular) Antibody
Brand: NeuroMab
SKU: 75-221
Price: 329.0 USD
Availability: InStock

20 Citations

Our Anti-LRP4 (extracellular) mouse monoclonal primary antibody from NeuroMab is produced in-house from hybridoma clone N207/27. It detects mouse and rat LRP4 (extracellular), and is purified by Protein A chromatography. It is great for use in ICC, WB.

Representative images of EDL muscles of 3 M, 12 M, and 24 M-old mice, whole mount stained with 555-anti-LRP4 (Cat no 75-221, 1:200, red) and NF/Syn (blue). Arrowheads, non-annulospiral axons had no LRP4 expression. Arrows, LRP4 negative annulospiral endings in aged mice. Scale bar, 10 µm. Image from publication CC-BY-4.0. PMID:36765071

Western blot image of mouse skeletal muscle confirming of expression of Flag-LRP4 (catalog no 75-221, 1:1000). Image from publication CC-BY-4.0. PMID:36765071

Rat ColQ−/− muscle cells were transduced to express ColQ-Myc (+) or not (−). After differentiation, the myotubes were treated (+) or not (−) with 10 nM of neural agrin for 1 h, and MuSK tyrosine phosphorylation was assessed as aforementioned. E, MuSK and LRP4 (cat. 75-221, 1:1000) cs levels of ColQ−/− myotubes re-expressing ColQ-Myc (+) or not (−) and not treated with agrin were analyzed. Image from publication CC-BY-4.0. PMID:37356721

Adult rat brain membrane immunoblot

Species Reactivity

Human, Mouse, Rabbit, Rat

Applications

ICC, IHC, WB

Host Species

Mouse

Available Conjugates

490

550

594

650

Go to Full Product Details

Data Sheet

SKU: 75-221

Volume: 100 µL

Variant

Price:

$329.00

Quantity:

Product Details

Target

LRP4 (extracellular)

Target Description

Low-density lipoprotein receptor-related protein 4 or LRP4 is encoded by the gene LRP4. And is a member of the low-density lipoprotein receptor-related protein family. Proteins in this family function in a wide range of physiological processes, including the regulation of lipid metabolism, protection against atherosclerosis, neurodevelopment. LRP4 may be involved in the negative regulation of the canonical Wnt signaling pathway. LRP4 is expressed in bone and several regions of the brain. Mutations in this gene are associated with Cenani-Lenz syndrome.

Format

Purified by Protein A chromatography

Concentration

1 mg/mL

Clonality

Monoclonal

Clone

N207/27

Isotype

IgG2a

Applications

ICC, IHC, WB

Host Species

Mouse

Gene Name

Lrp4 Kiaa0816

Molecular Weight

215 kDa

Antigen

Fusion protein amino acids 26-350 (extracellular N-terminus) of mouse LRP4 (accession number Q8VI56) produced recombinantly in E. Coli

Species Reactivity

Human, Mouse, Rabbit, Rat

Antibody Registry ID

AB_2139030

Storage

Aliquot and store at ≤ -20°C for long term storage. For short term storage, store at 2-8°C. For maximum recovery of product, centrifuge the vial prior to removing the cap.

Physical State

Liquid

Production Notes

Produced by in vitro bioreactor culture of hybridoma line followed by Protein A affinity chromatography. Purified mAbs are >90% specific antibody.

Buffer

10 mM Tris, 50 mM Sodium Chloride, 0.065% Sodium Azide pH 7.125

Dilution Ranges

WB: 1:1000
IHC: 1:500

Conjugate

Unconjugated

Specificity

No cross-reactivity reported

Quality Control

Each new lot of antibody is quality control tested by western blot on rat whole brain lysate and confirmed to stain the expected molecular weight band.

Usage Statement

These antibodies are to be used as research laboratory reagents and are not for use as diagnostic or therapeutic reagents in humans.

Country of Origin

United States

Expiration

24 months from date of receipt

Shipping

Shipped on ice packs

Synonyms

Low-density lipoprotein receptor-related protein 4 (LRP-4) (LDLR dan)

UniProt Details

UniProt (Human): Q8VI56
UniProt (Immunogen Species): Q8VI56

Product Specific References for Applications and Species

Immunohistochemistry: Mouse
Patch Clamp Recording: Mouse
Solid Phase Binding Assay: Human
Western Blot: Mouse | Rabbit | Rat
Additional Publications: Unspecified

Immunohistochemistry: Mouse
PMID	Dilution	Publication
36765071	1:200	Cao, R., et al. 2023. Intrafusal-fiber LRP4 for muscle spindle formation and maintenance in adult and aged animals. Nature Communications, 744.
31268420	1:1000	Zhang, H., et al. 2019. Agrin-Lrp4-Ror2 signaling regulates adult hippocampal neurogenesis in mice.. Elife, e45303.

Patch Clamp Recording: Mouse
PMID	Dilution	Publication
24462100	not listed	Wang, K., et al. 2014. Distinct Ca2+ sources in dendritic spines of hippocampal CA1 neurons couple to SK and Kv4 channels.. Neuron, 379-387.

Solid Phase Binding Assay: Human
PMID	Dilution	Publication
34233932	not listed	Yu, Z., et al. 2021. Characterization of LRP4/agrin antibodies from a patient with myasthenia gravis. Neurology, e975-e987.

Western Blot: Mouse
PMID	Dilution	Publication
36765071	1:1000	Cao, R., et al. 2023. Intrafusal-fiber LRP4 for muscle spindle formation and maintenance in adult and aged animals. Nature Communications, 744.
34090516	1:500	Jing, H., et al. 2021. Synapse-specific Lrp4 mRNA enrichment requires Lrp4/MuSK signaling, muscle activity and Wnt non-canonical pathway. Cell Bioscience, 105.
34063992	1:1000	Hui, T.K., et al. 2021. Ablation of Lrp4 in Schwann cells promotes peripheral nerve regeneration in mice. Biology (Basel), 452.
33987657	1:1000	Hui, T., et al. 2021. Increasing LRP4 diminishes neuromuscular deficits in a mouse model of Duchenne muscular dystrophy. Human Molecular Genetics, 1579-1590.
32687671	1:1000	Chen, A., et al. 2020. APC2 CDH1 negatively regulates agrin signaling by promoting the ubiquitination and proteolytic degradation of DOK7. FASEB, 12009-12023.
32457076	1:1000	Zhang, H., et al. 2020. A role of low-density lipoprotein receptor-related protein 4 (LRP4) in astrocytic Aβ clearance.. Journal of Neuroscience, 5347-5361.
31261376	1:1000	Xiao, Y., et al. 2020. Loss of mitochondrial protein CHCHD10 in skeletal muscle causes neuromuscular junction impairment. Human Molecular Genetics, 1784-1796.
28193701	not listed	Xiong, L., et al. 2017. Osteoblastic Lrp4 promotes osteoclastogenesis by regulating ATP release and adenosine-A2AR signaling.. The Journal of Biology, 761-778.
27294513	1:1000	Sun, X.D., et al. 2016. Lrp4 in astrocytes modulates glutamatergic transmission. Nature Neuroscience, 1010-1018.
25407677	1:2500	Gomez, A.M., et al. 2014. Synaptic plasticity and cognitive function are disrupted in the absence of Lrp4.. Elife, e04287.
25319686	1:1000	Barik, A., et al. 2013. LRP4 is critical for neuromuscular junction maintenance.. Journal of Neuroscience, 13892-13905.
24200689	1:1000	Shen, C., et al. 2013. Antibodies against low-density lipoprotein receptor–related protein 4 induce myasthenia gravis. Journal of Clinical Investigation, 5190-5202.
22794264	1:1000	Wu, H., et al. 2012. Distinct roles of muscle and motoneuron LRP4 in neuromuscular junction formation.. Neuron, 94-107.

Western Blot: Rabbit
PMID	Dilution	Publication
24200689	1:1000	Shen, C., et al. 2013. Antibodies against low-density lipoprotein receptor–related protein 4 induce myasthenia gravis. Journal of Clinical Investigation, 5190-5202.

Western Blot: Rat
PMID	Dilution	Publication
37356721	1:1000	Uyen Dao, TM, et al. 2023. The Collagen ColQ Binds to LRP4 and Regulates the Activation of the Muscle-Specific Kinase/LRP4 Receptor Complex by Agrin at the Neuromuscular Junction. The Journal of Biological Chemistry, 104962.

Additional Publications: Unspecified
PMID	Publication
38734896	Sun, S, et al. 2024. The MuSK agonist antibody protects the neuromuscular junction and extends the lifespan in C9orf72-ALS mice. Molecular Therapy, 0.

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Anti-LRP4 (Extracellular) Antibody (N207/27)

SKU: 75-221

Product Details

Product Specific References for Applications and Species

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