Anti-Mad3 Antibody (N129A/6)

Name: Anti-Mad3 Antibody
Brand: NeuroMab
SKU: 75-227
Price: 319.0 USD
Availability: InStock

Our Anti-Mad3 mouse monoclonal primary antibody from NeuroMab is produced in-house from hybridoma clone N129A/6. It detects human and mouse Mad3, and is purified by Protein A chromatography. It is great for use in ICC, WB.

Transfected cell immunoblot: extracts of COS cells transiently transfected with HA-tagged mouse and human Mad3 (mMad3 and hMad3, respectively) and untagged Kv2.1 plasmids and probed with N129A/6 (left) and K89/41 (right) TC supes.

Species Reactivity

Human, Mouse

Applications

ICC, WB

Host Species

Mouse

Available Conjugates

490

550

594

650

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Data Sheet

SKU: 75-227

Volume: 100 µL

Variant

Price:

$319.00

Quantity:

Product Details

Target

Mad3

Target Description

MAX Dimerization Protein 3 is encoded by the gene MXD3. MXD3 is a member of the Myc superfamily. MXD3 is a transcriptional repressor which forms a heterodimer with the cofactor MAX to form a sequence-specific DNA-binding protein complex which recognizes the core sequence 5'-CAC[GA]TG-3'. MXD3 is expressed in lung and blood. Diseases associated with MXD3 include Perianal Hematoma and Bartholin's Duct Cyst.

Format

Purified by Protein A chromatography

Concentration

1 mg/mL

Clonality

Monoclonal

Clone

N129A/6

Isotype

IgG2b

Applications

ICC, WB

Host Species

Mouse

Gene Name

MXD3 BHLHC13 MAD3

Molecular Weight

25 kDa

Antigen

Fusion protein amino acids 116-206 (C-terminus) of human Mad3 (accession number Q9BW11) produced recombinantly in E. Coli

Species Reactivity

Human, Mouse

Antibody Registry ID

AB_2147865

Storage

Aliquot and store at ≤ -20°C for long term storage. For short term storage, store at 2-8°C. For maximum recovery of product, centrifuge the vial prior to removing the cap.

Physical State

Liquid

Production Notes

Produced by in vitro bioreactor culture of hybridoma line followed by Protein A affinity chromatography. Purified mAbs are >90% specific antibody.

Buffer

10 mM Tris, 50 mM Sodium Chloride, 0.065% Sodium Azide pH 7.125

Conjugate

Unconjugated

Specificity

No cross-reactivity reported

Quality Control

Each new lot of antibody is quality control tested on cells overexpressing target protein and confirmed to give the expected staining pattern.

Usage Statement

These antibodies are to be used as research laboratory reagents and are not for use as diagnostic or therapeutic reagents in humans.

Country of Origin

United States

Expiration

24 months from date of receipt

Shipping

Shipped on ice packs

Synonyms

Max dimerization protein 3 (Max dimerizer 3) (Class C basic helix-loop-helix protein 13) (bHLHc13) (Max-associated protein 3) (Max-interacting transcriptional repressor MAD3) (Myx)

UniProt Details

UniProt (Human): Q9BW11
UniProt (Immunogen Species): Q9BW11

Product Specific References for Applications and Species

Immunocytochemistry: Human
Western Blot: Human

Immunocytochemistry: Human
PMID	Dilution	Publication
31783139	not listed	Yoshida, S., et al. 2019. MXD3 antisense oligonucleotide with superparamagnetic iron oxide nanoparticles: A new targeted approach for neuroblastoma. Nanomedicine, 102127.

Western Blot: Human
PMID	Dilution	Publication
31783139	not listed	Yoshida, S., et al. 2019. MXD3 antisense oligonucleotide with superparamagnetic iron oxide nanoparticles: A new targeted approach for neuroblastoma. Nanomedicine, 102127.

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Anti-Mad3 Antibody (N129A/6)

SKU: 75-227

Product Details

Product Specific References for Applications and Species

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