Anti-Mitofusin-2 Antibody (N153/5)

Name: Anti-Mitofusin-2 Antibody
Brand: NeuroMab
SKU: 75-173
Price: 319.0 USD
Availability: InStock

Our Anti-Mitofusin-2 mouse monoclonal primary antibody from NeuroMab is produced in-house from hybridoma clone N153/5. It is KO validated, detects mouse and rat Mitofusin-2, and is purified by Protein A chromatography. It is great for use in IHC, ICC, WB.

Immunoblot versus crude cardiac mitochondrial preps from WT, Mfn1 KO or Mfn2 KO mice and probed with N153/5 TC supe (top) and Porin antibody (bottom). Data courtesy of Dr. Suzanne Hoppins, Dr. Laura Lackner and Dr. Jodi Nunnari, UCDavis.

Adult rat hippocampus immunohistochemistry.

Species Reactivity

Mouse, Rat

Applications

ICC, IHC, WB

Host Species

Mouse

Available Conjugates

490

550

594

650

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Data Sheet

KO Validated

SKU: 75-173

Volume: 100 µL

Variant

Price:

$319.00

Quantity:

Product Details

Target

Mitofusin-2

Target Description

Mitofusin 2, or transmembrane GTPase Mfn2 (MFN2) is a mitochondrial membrane protein with GTPase activity that functions to aid mitochondrial clustering and fusion. It may also play a role in mitochondrial metabolism. It is expressed in the outer membrane of mitochondria. MFN2 is expressed in many tissues including the heart, skeletal muscle, tongue, and brain. Mutations in this gene cause Charcot-Marie-Tooth disease type 2A2, and hereditary motor and sensory neuropathy VI,

Format

Purified by Protein A chromatography

Concentration

1 mg/mL

Clonality

Monoclonal

Clone

N153/5

Isotype

IgG2a

Applications

ICC, IHC, WB

Host Species

Mouse

Gene Name

Mfn2 Kiaa0214 Marf

Molecular Weight

90 kDa

Antigen

Fusion protein amino acids 370-600 (cytoplasmic N-terminus) of mouse Mitofusin-2 (accession number Q80U63) produced recombinantly in E. Coli

Species Reactivity

Mouse, Rat

Antibody Registry ID

AB_2297598

Storage

Aliquot and store at ≤ -20°C for long term storage. For short term storage, store at 2-8°C. For maximum recovery of product, centrifuge the vial prior to removing the cap.

Physical State

Liquid

Production Notes

Produced by in vitro bioreactor culture of hybridoma line followed by Protein A affinity chromatography. Purified mAbs are >90% specific antibody.

Buffer

10 mM Tris, 50 mM Sodium Chloride, 0.065% Sodium Azide pH 7.101

Conjugate

Unconjugated

Specificity

No cross-reactivity against Mitofusin-1

Quality Control

Each new lot of antibody is quality control tested on cells overexpressing target protein and confirmed to give the expected staining pattern.

Usage Statement

These antibodies are to be used as research laboratory reagents and are not for use as diagnostic or therapeutic reagents in humans.

Country of Origin

United States

Expiration

24 months from date of receipt

Shipping

Shipped on ice packs

Synonyms

Mitofusin-2 (EC 3.6.5.-) (Hypertension-related protein 1) (Mitochondrial assembly regulatory factor) (HSG protein) (Transmembrane GTPase MFN2)

UniProt Details

UniProt (Human): O95140
UniProt (Immunogen Species): Q80U63

Product Specific References for Applications and Species

Western Blot: Mouse

Western Blot: Mouse
PMID	Dilution	Publication
34152608	1:500	López‐Doménech, G., et al. 2021. Loss of neuronal Miro1 disrupts mitophagy and induces hyperactivation of the integrated stress response. . The EMBO journal, e100715.
21255726	not listed	Hoppins, S., et al. 2011. The soluble form of Bax regulates mitochondrial fusion via MFN2 homotypic complexes.. Molecular Cell, 150-160.

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Anti-Mitofusin-2 Antibody (N153/5)

SKU: 75-173

Product Details

Product Specific References for Applications and Species

Bulk Order Anti-Mitofusin-2 Antibody

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