Anti-Nav1.2 Na+ Channel Antibody (K69/3)
Our Anti-Nav1.2 Na+ channel mouse monoclonal primary antibody from NeuroMab is produced in-house from hybridoma clone K69/3. It detects feline, human, mouse, non-human primate, and rat Nav1.2 Na+ channel, and is purified by Protein A chromatography. It is great for use in IHC, ICC, IP, WB.
![Immunoblot against crude membrane fractions from whole mouse (MBM) or rat (RBM) brain and from human hippocampus [HBM(H)], cerebral cortex [HBM(Cx)] or cerebellum [HBM(Cb)] and probed with K69/3 (left) or N52A/42 (right) TC supe.](http://www.antibodiesinc.com/cdn/shop/files/73-024-k69-3-wb-1_{width}x.jpg?v=1703182493)
Adult rat whole brain immunohistochemistry.
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![Immunoblot against crude membrane fractions from whole mouse (MBM) or rat (RBM) brain and from human hippocampus [HBM(H)], cerebral cortex [HBM(Cx)] or cerebellum [HBM(Cb)] and probed with K69/3 (left) or N52A/42 (right) TC supe.](http://www.antibodiesinc.com/cdn/shop/files/73-024-k69-3-wb-1_1024x.jpg?v=1703182493)
Feline, Human, Mouse, Non-Human Primate, Rat
ICC, IHC, WB
Mouse
Ships: 1-2 business days
Product Specific References for Applications and Species
- FRET: Rat
- Immunocytochemistry: Human | Mouse | Rat
- Immunohistochemistry: Feline | Human | Mouse | Non-Human Primate | Rat
- Immunoprecipitation: Rat
- STORM: Rat
- Western Blot: Mouse | Rat
- Additional Publications: Unspecified
| Fluorescence Resonance Energy Transfer: Rat | ||
| PMID | Dilution | Publication |
| 28029095 | not listed | Plant, L.D., et al. 2016. SUMOylation of NaV1. 2 channels mediates the early response to acute hypoxia in central neurons. . Elife, 20054. |
| Immunocytochemistry: Human | ||
| PMID | Dilution | Publication |
| 39804991 | 1:500 | Wang, H, et al. 2025. The Primary Cilia are Associated with the Axon Initial Segment in Neurons. Advanced science (Weinheim, Baden-Wurttemberg, Germany), e2407405. |
| 19806359 | not listed | Merrick, E.C., et al. 2010. The importance of serine 161 in the sodium channel beta3 subunit for modulation of Na(V)1.2 gating.. Pflügers Archiv: European Journal of Physiology, 743-753. |
| Immunocytochemistry: Mouse | ||
| PMID | Dilution | Publication |
| 39415847 | 1:500 | Li, Y, et al. 2024. Alterations in the axon initial segment plasticity is involved in early pathogenesis in Alzheimer's disease. MedComm, e768. |
| 34534742 | not listed | Wang, Y., et al. 2021. The largest isoform of Ankyrin-G is required for lattice structure of the axon initial segment.. Biochemical and Biophysical Research Communications, 28-34. |
| 33192291 | not listed | Smalley, J.L., et al. 2020. Isolation and characterization of multi-protein complexes enriched in the K-Cl Co-transporter 2 from brain plasma membranes. . Frontiers in Molecular Neuroscience, 190. |
| 32592656 | 1:1000 | Shemesh, O.A., et al. 2020. Precision calcium imaging of dense neural populations via a cell-body-targeted calcium indicator. . Neuron, 470-486. |
| 31186137 | not listed | Wang, B., et al. 2019. Cilia function is associated with axon initial segment morphology.. Biochemical and Biophysical Research Communications, 15-21. |
| 28931427 | not listed | Hu, L., et al. 2017. Serotonin 5-HT6 receptors affect cognition in a mouse model of Alzheimer's disease by regulating cilia function.. Alzheimers Research and Therapy, 76. |
| 20133873 | 1:100 | Brackenbury, W.J., et al. 2010. Functional reciprocity between Na+ channel Nav1. 6 and β1 subunits in the coordinated regulation of excitability and neurite outgrowth. . Proceedings of the National Academy of Sciences, 2283-2288. |
| 19013247 | 1:100-1:250 | O'Malley, H.A., et al. 2009. Loss of Na+ channel β2 subunits is neuroprotective in a mouse model of multiple sclerosis. . Molecular and Cellular Neuroscience, 143-155. |
| Immunocytochemistry: Rat | ||
| PMID | Dilution | Publication |
| 23349866 | 1:200 | He, Y.L., et al. 2013. Exposure to extremely low-frequency electromagnetic fields modulates Na+ currents in rat cerebellar granule cells through increase of AA/PGE2 and EP receptor-mediated cAMP/PKA pathway.. PLoS One, e54376. |
| 20045450 | not listed | Conant, K., et al. 2010. Matrix metalloproteinase-dependent shedding of intercellular adhesion molecule-5 occurs with long-term potentiation.. Neuroscience, 508-521. |
| 19603521 | 1:100 | Kress, G.J., et al. 2010. Axonal sodium channel distribution shapes the depolarized action potential threshold of dentate granule neurons.. Hippocampus, 558-571. |
| 18953054 | 1:100 | Lonigro, A., et al. 2009. Disruption of neurofascin and gliomedin at nodes of Ranvier precedes demyelination in experimental allergic neuritis.. Brain, 260-273. |
| Immunohistochemistry: Feline | ||
| PMID | Dilution | Publication |
| 33449375 | 1:1000 | Deardorff, A.S., et al. 2021. Location, location, location: the organization and roles of potassium channels in mammalian motoneurons.. Journal of Physiology, 1391-1420. |
| Immunohistochemistry: Human | ||
| PMID | Dilution | Publication |
| 20147455 | 1:100 | Black, J.A., et al. 2010. Astrocytes within multiple sclerosis lesions upregulate sodium channel Nav1. 5. . Brain, 835-846. |
| 18442883 | 1:100 | Zhao, P., et al. 2008. Voltage-gated sodium channel expression in rat and human epidermal keratinocytes: evidence for a role in pain. . Pain, 90-105. |
| Immunohistochemistry: Mouse | ||
| PMID | Dilution | Publication |
| 35259014 | not listed | Jo, Y., et al. 2022. Poly-dipeptides produced from C9orf72 hexanucleotide repeats cause selective motor neuron hyperexcitability in ALS.. PNAS: USA, e2113813119. |
| 34850743 | 1:500 | Li, M., et al. 2021. Antisense oligonucleotide therapy reduces seizures and extends life span in an SCN2A gain-of-function epilepsy model.. The Journal of Clinical Investigation, 23. |
| 34496232 | 1:400 | Gould, E., et al. 2021. SCN2A contributes to oligodendroglia excitability and development in the mammalian brain.. Cell Reports, 109653. |
| 33449375 | 1:1000 | Deardorff, A.S., et al. 2021. Location, location, location: the organization and roles of potassium channels in mammalian motoneurons.. Journal of Physiology, 1391-1420. |
| 31933626 | 1:300 | Alshammari, M.A., et al. 2019. Changes in the Fluorescence Tracking of NaV1.6 Protein Expression in a BTBR T+Itpr3tf/J Autistic Mouse Model.. Neural Plasticity, 4893103. |
| 31354436 | 1:200 | Yang, J., et al. 2019. Biophysical properties of somatic and axonal voltage-gated sodium channels in midbrain dopaminergic neurons. . Frontiers in Cellular Neuroscience, 17. |
| 31201232 | 1:200 | Hosoi, N., et al. 2019. Deletion of Class II ADP-Ribosylation Factors in Mice Causes Tremor by the Nav1.6 Loss in Cerebellar Purkinje Cell Axon Initial Segments.. Journal of Neuroscience, 6339-6353. |
| 30125271 | 1:1000 | Nunes, D., et al. 2018. Axonal sodium channel NaV1. 2 drives granule cell dendritic GABA release and rapid odor discrimination. . PLoS Biology, e2003816. |
| 26909021 | 1:300 | Alshammari, M.A., et al. 2016. Improved methods for fluorescence microscopy detection of macromolecules at the axon initial segment. . Frontiers in Cellular Neuroscience, 5. |
| 25948275 | 1:100 | Hamada, M.S. , et al. 2015. Myelin loss and axonal ion channel adaptations associated with gray matter neuronal hyperexcitability.. Journal of Neuroscience, 7272-7286. |
| 25378553 | 1:300 | Gazina, E.V., et al. 2015. Neonatal'' Nav1.2 reduces neuronal excitability and affects seizure susceptibility and behaviour.. Human Molecular Genetics, 1457-1468. |
| 25085892 | 1 : 500 | Wang, X., et al. 2014. Thorough GABAergic innervation of the entire axon initial segment revealed by an optogenetic ''laserspritzer''.. The Journal of Physiology, 4257-4276. |
| 24077057 | 1:1500 | Vega, A.V., et al. 2013. Interaction between the transcriptional corepressor Sin3B and voltage-gated sodium channels modulates functional channel expression. . Scientific Reports, 1-8. |
| 21676499 | 1:300 | Wykes, R., et al. 2012. Changes in the physiology of CA1 hippocampal pyramidal neurons in preplaque CRND8 mice. . Neurobiology of Aging, 1609-1623. |
| 20628201 | not listed | Wimmer, V.C., et al. 2010. Axon initial segment dysfunction in a mouse model of genetic epilepsy with febrile seizures plus. . The Journal of Clinical Investigation, 2661-2671. |
| 20375142 | not listed | Wimmer, V.C., et al. 2010. Axon initial segment dysfunction in epilepsy. . The Journal of Physiology, 1829-1840. |
| 20371507 | 1:500 | Liao, Y., et al. 2010. Molecular correlates of age-dependent seizures in an inherited neonatal-infantile epilepsy.. Brain, 1403-1414. |
| Immunohistochemistry: Non-Human Primate | ||
| PMID | Dilution | Publication |
| 34496232 | 1:400 | Gould, E., et al. 2021. SCN2A contributes to oligodendroglia excitability and development in the mammalian brain.. Cell Reports, 109653. |
| Immunohistochemistry: Rat | ||
| PMID | Dilution | Publication |
| 33449375 | 1:1000 | Deardorff, A.S., et al. 2021. Location, location, location: the organization and roles of potassium channels in mammalian motoneurons.. Journal of Physiology, 1391-1420. |
| 31223136 | 1:100 | Effraim, P.R., et al. 2019. Fibroblast growth factor homologous factor 2 (FGF-13) associates with Nav1. 7 in DRG neurons and alters its current properties in an isoform-dependent manner.. Neurobiology of Pain, 100029. |
| 28916793 | 1:50 | Berret, E., et al. 2017. Oligodendroglial excitability mediated by glutamatergic inputs and Nav1.2 activation.. Nature Communications, 1-15. |
| 25294986 | 1:400 | Tian, C., et al. 2014. Molecular identity of axonal sodium channels in human cortical pyramidal cells. . Frontiers in Cellular Neuroscience, 297. |
| 24077057 | 1:1500 | Vega, A.V., et al. 2013. Interaction between the transcriptional corepressor Sin3B and voltage-gated sodium channels modulates functional channel expression. . Scientific Reports, 1-8. |
| 22473424 | 1 : 500 | Huang, C.Y., et al. 2012. Coexpression of high-voltage-activated ion channels Kv3.4 and Cav1.2 in pioneer axons during pathfinding in the developing rat forebrain.. Journal of Comparative Neurology, 3650-3672. |
| 21734302 | 1:200 | Lysakowski, A., et al. 2011. Molecular microdomains in a sensory terminal, the vestibular calyx ending.. Journal of Neuroscience, 10101-10114. |
| 21569247 | 1:100 | Ahn, H.S., et al. 2011. Nav1. 7 is the predominant sodium channel in rodent olfactory sensory neurons. . Molecular Pain, 1744-8069. |
| 18769561 | 1:500-1:1000 | Mojumder, D.K., et al. 2008. Subcellular compartmentalization of two calcium binding proteins, calretinin and calbindin-28 kDa, in ganglion and amacrine cells of the rat retina. . Molecular Vision, 1600. |
| Immunoprecipitation: Rat | ||
| PMID | Dilution | Publication |
| 24737319 | not listed | Baek, J.H., et al. 2014. Reciprocal changes in phosphorylation and methylation of mammalian brain sodium channels in response to seizures.. The Journal of Biological Chemistry, 15363-15373. |
| 20131913 | 2.5 μg/ml | Berendt, F.J., et al. 2010. Multisite phosphorylation of voltage-gated sodium channel alpha subunits from rat brain.. Journal of Proteome Research, 1976-1984. |
| STORM: Rat | ||
| PMID | Dilution | Publication |
| 28029095 | not listed | Plant, L.D., et al. 2016. SUMOylation of NaV1. 2 channels mediates the early response to acute hypoxia in central neurons. . Elife, 20054. |
| Western Blot: Mouse | ||
| PMID | Dilution | Publication |
| 34156984 | 1:200 | Wang, H.G., et al. 2021. Scn2a severe hypomorphic mutation decreases excitatory synaptic input and causes autism-associated behaviors.. JCI Insight, e150698. |
| 31933626 | 1:500 | Alshammari, M.A., et al. 2019. Changes in the Fluorescence Tracking of NaV1.6 Protein Expression in a BTBR T+Itpr3tf/J Autistic Mouse Model.. Neural Plasticity, 4893103. |
| 31920555 | 1:200 | Mi, Z., et al. 2019. Alterations of electrophysiological properties and ion channel expression in prefrontal cortex of a mouse model of schizophrenia. . Frontiers in Cellular Neuroscience, 554. |
| 31308096 | 1:1000 | Gomez, C.D., et al. 2019. Early life inflammation increases CA1 pyramidal neuron excitability in a sex and age dependent manner through a chloride homeostasis disruption. . Journal of Neuroscience, 7244-7259. |
| 31249508 | 1:500 | Shin, W., et al. 2019. Scn2a haploinsufficiency in mice suppresses hippocampal neuronal excitability, excitatory synaptic drive, and long-term potentiation, and spatial learning and memory. . Frontiers in Molecular Neuroscience, 145. |
| 30125271 | not listed | Nunes, D., et al. 2018. Axonal sodium channel NaV1. 2 drives granule cell dendritic GABA release and rapid odor discrimination. . PLoS Biology, e2003816. |
| 29491011 | 1:200 | Zhou, L., et al. 2018. Celecoxib ameliorates seizure susceptibility in autosomal dominant lateral temporal epilepsy. . Journal of Neuroscience, 3346-3357. |
| 28238546 | 1:1000 | Makinson, C.D., et al. 2017. Regulation of thalamic and cortical network synchrony by Scn8a. . Neuron, 1165-1179. |
| 26889602 | 1:2000 | Bosch, M.K., et al. 2016. Proteomic analysis of native cerebellar iFGF14 complexes.. Channels, 297-312. |
| 25203314 | 1:500 | Li, T., et al. 2014. Action potential initiation in neocortical inhibitory interneurons. . PLoS Biology, e1001944. |
| 24704313 | 1:1000 | Makinson, C.D., et al. 2014. Role of the hippocampus in Nav1. 6 (Scn8a) mediated seizure resistance. . Neurobiology of Disease, 16-25. |
| 22131424 | 1:500 | Kaphzan, H., et al. 2011. Alterations in intrinsic membrane properties and the axon initial segment in a mouse model of Angelman syndrome.. Journal of Neuroscience, 17637-17648. |
| 19013247 | 1:100-1:250 | O'Malley, H.A., et al. 2009. Loss of Na+ channel β2 subunits is neuroprotective in a mouse model of multiple sclerosis. . Molecular and Cellular Neuroscience, 143-155. |
| Western Blot: Rat | ||
| PMID | Dilution | Publication |
| 28871036 | not listed | Cho, I.H., et al. 2017. Sodium channel β2 subunits prevent action potential propagation failures at axonal branch points. . Journal of Neuroscience, 9519-9533. |
| 26494800 | 1:200 | Yin, L., et al. 2017. Selective Modulation of Axonal Sodium Channel Subtypes by 5-HT1A Receptor in Cortical Pyramidal Neuron.. Cereb Cortex, 509-521. |
| 20067584 | not listed | Bouvier, D., et al. 2010. EphA4 is localized in clathrin-coated and synaptic vesicles in adult mouse brain.. Journal of Neurochemistry, 153-165. |
| 19692609 | 1:1000 | von Reyn, C.R., et al. 2009. Calpain mediates proteolysis of the voltage-gated sodium channel α-subunit. . Journal of Neuroscience, 10350-10356. |
| Additional Publications: Unspecified | ||
| PMID | Publication | |
| 25421039 | Wimmer, V.C., et al. 2015. Sodium channel β1 subunit localizes to axon initial segments of excitatory and inhibitory neurons and shows regional heterogeneity in mouse brain.. Journal of Comparative Neurology, 814-830. | |
| 24681347 | Liu, W., et al. 2014. KCNE2 modulates cardiac L-type Ca(2+) channel.. Journal of Molecular and Cellular Cardiology, 208-218. | |
| 22528969 | Martínez-Hernández, J., et al. 2013. Polarised localisation of the voltage-gated sodium channel Na(v)1.2 in cerebellar granule cells.. The Cerebellum, 16-26. | |
| 22473675 | Dhaunchak, A.S., et al. 2012. Implication of perturbed axoglial apparatus in early pediatric multiple sclerosis.. Annals of Neurology, 601-613. | |
| 19800949 | Crawford, D.K., et al. 2009. Functional recovery of callosal axons following demyelination: a critical window.. Neuroscience, 1407-1421. | |
| 19743470 | Thomas, E.A., et al. 2009. Heat opens axon initial segment sodium channels: a febrile seizure mechanism?. Annals of Neurology, 219-226. | |
| 18601978 | Vega, A.V., et al. 2008. Reduced expression of Nav1. 6 sodium channels and compensation by Nav1. 2 channels in mice heterozygous for a null mutation in Scn8a. . Neuroscience letters, 69-73. | |
| 18079688 | Mojumder, D.K., et al. 2007. Voltage-gated sodium channel alpha-subunits Na(v)1.1, Na(v)1.2, and Na(v)1.6 in the distal mammalian retina.. Molecular Vision, 2163-2182. | |
