Our Anti-NSD1 mouse monoclonal primary antibody from NeuroMab is produced in-house from hybridoma clone N312/10. It detects human and mouse NSD1, and is purified by Protein A chromatography. It is great for use in ICC, WB.
Transfected cell immunoblot: extracts of COS cells transiently transfected with HA-tagged NSD1, NSD2, NSD3 or untagged Kv2.1 plasmids and probed with N312/10 (left), 12CA5 (middle) and K89/34 (right).
Nuclear Receptor Binding SET Domain Protein 1 is encoded by the gene NSD1. NSD1 is a member of the class V-like SAM-binding methyltransferase superfamily. NSD1 is a histone methyltransferase that is capable of both negatively or positively influencing transcription, depending on the cellular context. NSD1 is expressed in the brain, kidney, spleen, skeletal muscle, thymus, and lung. Diseases associated with NSD1 include Sotos Syndrome 1 and Overgrowth Syndrome.
Purified by Protein A chromatography
1 mg/mL
Monoclonal
N312/10
IgG2b
ICC, WB
Mouse
NSD1 ARA267 KMT3B
300 kDa
Fusion protein amino acids 2129-2696 (C-terminus) of human NSD1 (accession number Q96L73) produced recombinantly in E. Coli
Human, Mouse
AB_11001827
Aliquot and store at ≤ -20°C for long term storage. For short term storage, store at 2-8°C. For maximum recovery of product, centrifuge the vial prior to removing the cap.
Liquid
Produced by in vitro bioreactor culture of hybridoma line followed by Protein A affinity chromatography. Purified mAbs are >90% specific antibody.
10 mM Tris, 50 mM Sodium Chloride, 0.065% Sodium Azide pH 7.125
WB: 1:1000
Unconjugated
Does not cross-react with NSD2 or NSD3
Each new lot of antibody is quality control tested on cells overexpressing target protein and confirmed to give the expected staining pattern.
These antibodies are to be used as research laboratory reagents and are not for use as diagnostic or therapeutic reagents in humans.
United States
24 months from date of receipt
Shipped on ice packs
Histone-lysine N-methyltransferase, H3 lysine-36 and H4 lysine-20 specific (EC 2.1.1.43) (Androgen receptor coactivator 267 kDa protein) (Androgen receptor-associated protein of 267 kDa) (H3-K36-HMTase) (H4-K20-HMTase) (Lysine N-methyltransferase 3B) (Nuclear receptor-binding SET domain-containing protein 1) (NR-binding SET domain-containing protein)
Jevtic, Z., et al. 2022. SMARCA5 interacts with NUP98-NSD1 oncofusion protein and sustains hematopoietic cells transformation. Journal of experimental & clinical cancer research : CR, 34.
Drosos, Y., et al. 2022. NSD1 mediates antagonism between SWI/SNF and polycomb complexes and is required for transcriptional activation upon EZH2 inhibition. Molecular cell, .
Harutyunyan, A.S., et al. 2020. H3K27M in Gliomas Causes a One-Step Decrease in H3K27 Methylation and Reduced Spreading within the Constraints of H3K36 Methylation. Cell Reports, .
Satterlee, J.S., et al. 2015. Community resources and technologies developed through the NIH Roadmap Epigenomics Program.. Methods of Molecular Biology, 27-49.
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