Anti-Pan-Neurofascin (Extracellular) Antibody (A12/18)

Name: Anti-Pan-Neurofascin (Extracellular) Antibody
Brand: NeuroMab
SKU: 75-172
Price: 374.0 USD
Availability: InStock

64 Citations

Our Anti-Pan-Neurofascin (extracellular) mouse monoclonal primary antibody from NeuroMab is produced in-house from hybridoma clone A12/18. It detects human, mouse, non-human primate, and rat Pan-Neurofascin (extracellular), and is purified by Protein A chromatography. It is great for use in IHC, ICC, IP, WB.

$Immunoblot on crude membrane fractions from whole mouse (MBM) and rat (RBM) brain and from human hippocampus [HBM(H)], cerebral cortex [HBM(Cx)] and cerebellum [HBM(Cb)] probed with A12/18 (left) and N52A/42 (right) TC supe.$

Neurons transfected with TfR-SEP and mScarlet-NavII-III, fixed at DIV10, and stained with anti-neurofascin (NF) antibody (clone A12/18) and anti-mScarlet antibody. Intense staining of both mScarlet-NavII-III and NF defines the AIS. 18/20 transfected neurons (from 3 cultures) showed colocalization with endogenous NF. Image from publication CC-BY-4.0. PMID: 35978188

Biotinylated proteins colocalize with endogenous protein signals in the axon initial segment, soma, and somatodendrites. The proximity labeling was directed by anti-NFASC (cat. 75-172, 1:1000), anti-NeuN, and anti-MAP2 antibodies individually in DIV14 rat cortical neurons with one minute of reaction time. Image from publication CC-BY-4.0. PMID:38081810

Immunoblot on crude membrane fractions from whole mouse (MBM) and rat (RBM) brain and from human hippocampus [HBM(H)], cerebral cortex [HBM(Cx)] and cerebellum [HBM(Cb)] probed with A12/18 (left) and N52A/42 (right) TC supe.

Species Reactivity

Human, Mouse, Non-Human Primate, Rat

Applications

ICC, IHC, IP, WB

Host Species

Mouse

Available Conjugates

490

550

594

650

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Data Sheet

SKU: 75-172

Volume: 100 µL

Variant

Price:

$374.00

Quantity:

Product Details

Target

Pan-Neurofascin (extracellular)

Target Description

Neurofascin is encoded by the gene NFASC. Two major isoforms of NeuroFascin are expressed as a result of alternative splicing, NF186 and NF155. These proteins are expressed in the central nervous system. In brain, NF186 is expressed at Nodes of Ranvier andNF155 is expressed in unmyelinated axons. Neuofascin plays a role in cell adhesion and is thought to be involved in processes such as neurite extension, synaptogenesis and myelination. Diseases associated with this gene include Demyelinating Polyneuropathy.

Format

Purified by Protein A chromatography

Concentration

1 mg/mL

Clonality

Monoclonal

Clone

A12/18

Isotype

IgG2a

Applications

ICC, IHC, IP, WB

Host Species

Mouse

Gene Name

Nfasc

Molecular Weight

155, 186 kDa

Antigen

Lipid raft fractions isolated from adult rat brain. Target identified as Neurofascin (accession number P97685) by: -binding to NF-186-tranfected COS cells- recognition of NF155 and NF186 but not other highly related L1 CAMs like NrCAM- immunoprecipitation of NF155 and NF186 and confirmation by mass spectrometry - loss of immunostaining against NF186-shRNA knockdown samples Rat: amino acids 25-1110 (extracellular domain common to NF155 and NF186);

Species Reactivity

Human, Mouse, Non-Human Primate, Rat

Antibody Registry ID

AB_2282826

Storage

Aliquot and store at ≤ -20°C for long term storage. For short term storage, store at 2-8°C. For maximum recovery of product, centrifuge the vial prior to removing the cap.

Physical State

Liquid

Production Notes

Produced by in vitro bioreactor culture of hybridoma line followed by Protein A affinity chromatography. Purified mAbs are >90% specific antibody.

Buffer

10 mM Tris, 50 mM Sodium Chloride, 0.065% Sodium Azide pH 7.100

Dilution Ranges

WB: 1:1000
IHC: 1:500
ICC: 1:250

Conjugate

Unconjugated

Specificity

No cross-reactivity reported

Quality Control

Each new lot of antibody is quality control tested by western blot on rat whole brain lysate and confirmed to stain the expected molecular weight band.

Usage Statement

These antibodies are to be used as research laboratory reagents and are not for use as diagnostic or therapeutic reagents in humans.

Country of Origin

United States

Expiration

24 months from date of receipt

Shipping

Shipped on ice packs

Synonyms

Neurofascin NF-155 NF-186

UniProt Details

UniProt (Human): O94856
UniProt (Immunogen Species): P97685

Product Specific References for Applications and Species

Immunocytochemistry: Human | Mouse | Non-Human Primate | Rat
Immunohistochemistry: Human | Rat
Immunoproximity Labeling: Rat
Live Cell Imaging: Rat
Pulsed-pH Live Imaging: Rat
Western Blot: Human | Mouse | Rat
Additional Publications: Unspecified

Immunocytochemistry: Human
PMID	Dilution	Publication
35314674	1:100	Keren-Kaplan, T., et al. 2022. RUFY3 and RUFY4 are ARL8 effectors that promote coupling of endolysosomes to dynein-dynactin. Nature Communications, 1506.
31100061	1:50 (supe)	Guardia, C. M., et al. 2019. Reversible association with motor proteins (RAMP): A streptavidin-based method to manipulate organelle positioning. PLoS Biology, e3000279.
26671463	not listed	van Beuningen, S., et al. 2015. TRIM46 Controls Neuronal Polarity and Axon Specification by Driving the Formation of Parallel Microtubule Arrays. Neuron, 1208-26.
25283719	1:50-1:100	Notturno, F., et al. 2014. Autoantibodies to neurofascin-186 and gliomedin in multifocal motor neuropathy.. Journal of Neuroimmunology, 207-12.
24859753	1:100	Lukinavičius, G., et al. 2014. Fluorogenic probes for live-cell imaging of the cytoskeleton.. Nature Methods, 731-3.

Immunocytochemistry: Mouse
PMID	Dilution	Publication
35978188	1:500	Eichel, K., et al. 2022. Endocytosis in the axon initial segment maintains neuronal polarity. Nature, 128-135.
31328333	1:100	Dubessy, A. L., et al. 2019. Role of a Contactin multi‐molecular complex secreted by oligodendrocytes in nodal protein clustering in the CNS. Glia, 2248-63.
27098687	1:500	Fréal, A., et al. 2016. Cooperative Interactions between 480 kDa Ankyrin-G and EB Proteins Assemble the Axon Initial Segment. Journal of Neuroscience, 4421-33.
26947559	1:200	D'Este, E., et al. 2016. Subcortical cytoskeleton periodicity throughout the nervous system. Scientific Reports, 22741.
25950943	not listed	Papandréou, M. J., et al. 2015. CK2-regulated schwannomin-interacting protein IQCJ-SCHIP-1 association with AnkG contributes to the maintenance of the axon initial segment.. Journal of Neurochemistry, 527-37.

Immunocytochemistry: Non-Human Primate
PMID	Dilution	Publication
31100061	1:50 (supe)	Guardia, C. M., et al. 2019. Reversible association with motor proteins (RAMP): A streptavidin-based method to manipulate organelle positioning. PLoS Biology, e3000279.
22649224	not listed	Yap, C. C., et al. 2012. Doublecortin (DCX) mediates endocytosis of neurofascin independently of microtubule binding.. Journal of Neuroscience, 7439-53.

Immunocytochemistry: Rat
PMID	Dilution	Publication
38081810	1:1000	Zhang, W, et al. 2023. Immunoproximity biotinylation reveals the axon initial segment proteome. Nature Communications, 8201.
33571451	not listed	Zahavi, E. E., et al. 2021. Combined kinesin-1 and kinesin-3 activity drives axonal trafficking of TrkB receptors in Rab6 carriers. Developmental Cell, 494-508.e7.
32958558	not listed	Collura, K. M., et al. 2020. The palmitoyl acyltransferases ZDHHC5 and ZDHHC8 are uniquely present in DRG axons and control retrograde signaling via the Gp130/JAK/STAT3 pathway. Journal of Biological Chemistry, 15427-37.
32553155	1:1000	De Pace, R., et al. 2020. Synaptic Vesicle Precursors and Lysosomes Are Transported by Different Mechanisms in the Axon of Mammalian Neurons. Cell Reports, 107775.
32239080	not listed	Gerasimaitė, R., et al. 2020. Efflux pump insensitive rhodamine-jasplakinolide conjugates for G- and F-actin imaging in living cells. Organic & Biomolecular Chemistry, 2929-37.
31882453	1:100	Gaub, B. M., et al. 2020. Neurons differentiate magnitude and location of mechanical stimuli. Proceedings of the National Academy of Sciences, 848-856.
31727776	not listed	Torii, T., et al. 2020. NuMA1 promotes axon initial segment assembly through inhibition of endocytosis. Journal of Cell Biology, e201907048.
31658245	not listed	Ribeiro, L. F., et al. 2019. SorCS1-mediated sorting in dendrites maintains neurexin axonal surface polarization required for synaptic function. PLoS Biology, e3000466.
30967428	1:200	Harterink, M., et al. 2019. TRIM46 Organizes Microtubule Fasciculation in the Axon Initial Segment. Journal of Neuroscience, 4864-73.
30772082	not listed	Farías, G. G., et al. 2019. Feedback-Driven Mechanisms between Microtubules and the Endoplasmic Reticulum Instruct Neuronal Polarity. Neuron, 184-201.e8.
30598502	not listed	Hivert, B., et al. 2019. ADAM22 and ADAM23 modulate the targeting of the Kv1 channel-associated protein LGI1 to the axon initial segment. . Journal of Cell Science, jcs219774.
30016622	1ug/ml	Karasmanis, E.P., et al. 2018. Polarity of Neuronal Membrane Traffic Requires Sorting of Kinesin Motor Cargo during Entry into Dendrites by a Microtubule-Associated Septin. Developmental Cell, 204-218.
29395909	not listed	Berger, S.L., et al. 2018. Localized Myosin II Activity Regulates Assembly and Plasticity of the Axon Initial Segment. Neuron, 555-570.
28828584	not listed	Guo, Y., et al. 2017. Neurons with Multiple Axons Have Functional Axon Initial Segments. Neuroscience Bulletin, 641-52.
28559423	not listed	Ganguly, A., et al. 2017. Hsc70 chaperone activity is required for the cytosolic slow axonal transport of synapsin. Journal of Cell Biology, 2059-74.
28533267	not listed	Pinatel, D., et al. 2017. The Kv1-associated molecules TAG-1 and Caspr2 are selectively targeted to the axon initial segment in hippocampal neurons. Journal of Cell Science, 2209-2220.
28394337	not listed	Harterink, M., et al. 2017. DeActs: genetically encoded tools for perturbing the actin cytoskeleton in single cells. Nature Methods, 479-82.
27932952	not listed	Dumitrescu, A. S., et al. 2016. Evaluating Tools for Live Imaging of Structural Plasticity at the Axon Initial Segment. Frontiers in Cellular Neuroscience, 268.
27697928	not listed	Albrecht, D., et al. 2016. Nanoscopic compartmentalization of membrane protein motion at the axon initial segment.. The Journal of Biology, 37-46.
27559088	not listed	Guo, X., et al. 2016. Rab5 and its effector FHF contribute to neuronal polarity through dynein-dependent retrieval of somatodendritic proteins from the axon. Proceedings of the National Academy of Sciences, E5318-27.
27356871	not listed	Sohn, P. D., et al. 2016. Acetylated tau destabilizes the cytoskeleton in the axon initial segment and is mislocalized to the somatodendritic compartment.. Molecular Neurodegeneration, 47.
27220554	1:400	Sidenstein, S. C., et al. 2016. Multicolour Multilevel STED nanoscopy of Actin/Spectrin Organization at Synapses.. Scientific Reports, 26725.
26888935	1:100	Benned-Jensen, T., et al. 2016. Live Imaging of Kv7.2/7.3 Cell Surface Dynamics at the Axon Initial Segment: High Steady-State Stability and Calpain-Dependent Excitotoxic Downregulation Revealed.. Journal of Neuroscience, 2261-6.
26553875	1:100	Norkett, R., et al. 2016. DISC1-dependent Regulation of Mitochondrial Dynamics Controls the Morphogenesis of Complex Neuronal Dendrites.. The Journal of Biological Chemistry, 613-29.
26526995	not listed	Evans, M. D., et al. 2015. Rapid Modulation of Axon Initial Segment Length Influences Repetitive Spike Firing.. Cell Report, 1233-45.
26216902	not listed	Ganguly, A., et al. 2015. A dynamic formin-dependent deep F-actin network in axons.. The Journal of Biology, 401-417.
26019348	1:200	Franssen, E. H., et al. 2015. Exclusion of integrins from CNS axons is regulated by Arf6 activation and the AIS.. Journal of Neuroscience, 8359-75.
25950943	not listed	Papandréou, M. J., et al. 2015. CK2-regulated schwannomin-interacting protein IQCJ-SCHIP-1 association with AnkG contributes to the maintenance of the axon initial segment.. Journal of Neurochemistry, 527-37.
25561543	not listed	Freeman, S.A., et al. 2015. Acceleration of conduction velocity linked to clustering of nodal components precedes myelination. . Proceedings of the National Academy of Sciences, E321-E328.
24959118	not listed	Muir, J., et al. 2014. Plasticity of GABAA receptor diffusion dynamics at the axon initial segment.. Frontiers in Neuroscience, 151.
24711503	1:100	Jones, S. L., et al. 2014. Axon initial segment cytoskeleton comprises a multiprotein submembranous coat containing sparse actin filaments.. The Journal of Biology, 67-81.
22649224	not listed	Yap, C. C., et al. 2012. Doublecortin (DCX) mediates endocytosis of neurofascin independently of microtubule binding.. Journal of Neuroscience, 7439-53.

Immunohistochemistry: Human
PMID	Dilution	Publication
32289157	1:3000	Bekku, Y., et al. 2020. Independent anterograde transport and retrograde cotransport of domain components of myelinated axons. Journal of Cell Biology, e201906071.
26671463	not listed	van Beuningen, S., et al. 2015. TRIM46 Controls Neuronal Polarity and Axon Specification by Driving the Formation of Parallel Microtubule Arrays. Neuron, 1208-26.

Immunohistochemistry: Rat
PMID	Dilution	Publication
30772082	not listed	Farías, G. G., et al. 2019. Feedback-Driven Mechanisms between Microtubules and the Endoplasmic Reticulum Instruct Neuronal Polarity. Neuron, 184-201.e8.

Immunoproximity Labeling: Rat
PMID	Dilution	Publication
38081810	not listed	Zhang, W, et al. 2023. Immunoproximity biotinylation reveals the axon initial segment proteome. Nature Communications, 8201.

Live Cell Imaging: Rat
PMID	Dilution	Publication
36859689	not listed	Christensen, K.R., et al. 2023. Phosphomimetics at Ser199/Ser202/Thr205 in Tau Impairs Axonal Transport in Rat Hippocampal Neurons. Molecular Neurobiology, .

Pulsed-pH Live Imaging: Rat
PMID	Dilution	Publication
35978188	1:250	Eichel, K., et al. 2022. Endocytosis in the axon initial segment maintains neuronal polarity. Nature, 128-135.

Western Blot: Human
PMID	Dilution	Publication
31900387	1:500	Hamdan, H., et al. 2020. Mapping axon initial segment structure and function by multiplexed proximity biotinylation. Nature Communications, 100.
25378584	not listed	Jain, S., et al. 2015. Polarized sorting of the copper transporter ATP7B in neurons mediated by recognition of a dileucine signal by AP-1.. Molecular Biology of the Cell, 218-28.
22473675	not listed	Dhaunchak, A.S., et al. 2012. Implication of perturbed axoglial apparatus in early pediatric multiple sclerosis.. Annals of Neurology, 601-13.
20830807	not listed	Dhaunchak, A.S., et al. 2010. A proteome map of axoglial specializations isolated and purified from human central nervous system.. Glia, 1949-60.

Western Blot: Mouse
PMID	Dilution	Publication
36085162	1:10,000	Hatayama, M., et al. 2022. SLITRK1-Mediated Noradrenergic Projection Suppression in the Neonatal Prefrontal Cortex. Communications Biology, 935.
31910261	not listed	Lee, M., et al. 2020. Ecm29-mediated proteasomal distribution modulates excitatory GABA responses in the developing brain. Journal of Cell Biology, e201903033.
27098687	1:500	Fréal, A., et al. 2016. Cooperative Interactions between 480 kDa Ankyrin-G and EB Proteins Assemble the Axon Initial Segment. Journal of Neuroscience, 4421-33.
25950943	not listed	Papandréou, M. J., et al. 2015. CK2-regulated schwannomin-interacting protein IQCJ-SCHIP-1 association with AnkG contributes to the maintenance of the axon initial segment.. Journal of Neurochemistry, 527-37.

Western Blot: Rat
PMID	Dilution	Publication
28785724	1:1000	Kneynsberg, A., et al. 2017. Aging Does Not Affect Axon Initial Segment Structure and Somatic Localization of Tau Protein in Hippocampal Neurons of Fischer 344 Rats. eNeuro, ENEURO.0043-17.2017.
25950943	not listed	Papandréou, M. J., et al. 2015. CK2-regulated schwannomin-interacting protein IQCJ-SCHIP-1 association with AnkG contributes to the maintenance of the axon initial segment.. Journal of Neurochemistry, 527-37.

Additional Publications: Unspecified
PMID	Publication
27631998	Farías, G. G., et al. 2016. Imaging the Polarized Sorting of Proteins from the Golgi Complex in Live Neurons. Methods in Molecular Biology, 13-30.
25732815	D'Este, E., et al. 2015. STED nanoscopy reveals the ubiquity of subcortical cytoskeleton periodicity in living neurons.. Cell Report, 1246-51.
22632975	Galiano, M.R., et al. 2012. A distal axonal cytoskeleton forms an intra-axonal boundary that controls axon initial segment assembly.. Cell, 1125-39.
19001126	Hedstrom, K.L., et al. 2008. AnkyrinG is required for maintenance of the axon initial segment and neuronal polarity.. The Journal of Biology, 635-40.

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Anti-Pan-Neurofascin (Extracellular) Antibody (A12/18)

SKU: 75-172

Product Details

Product Specific References for Applications and Species

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