Anti-Pan-QKI Antibody (N147/6)
Our Anti-Pan-QKI mouse monoclonal primary antibody from NeuroMab is produced in-house from hybridoma clone N147/6. It detects human, mouse, and rat Pan-QKI, and is purified by Protein A chromatography. It is great for use in IHC, ICC, WB.
![Immunoblots on membranes prepared from whole mouse (MBM) and rat (RBM) brain, and from human hippocampus [HBM(H)], cerebral cortex [HBM(Cx)], and cerebellum [HBM(Cb)].](http://www.antibodiesinc.com/cdn/shop/products/validation-image-73-168-n147-6-wb3_61988529-2c8a-4bc7-a54a-e0675f05b9f3_{width}x.jpg?v=1626903796)
Immunoblots on membranes prepared from whole mouse (MBM) and rat (RBM) brain, and from human hippocampus [HBM(H)], cerebral cortex [HBM(Cx)], and cerebellum [HBM(Cb)].
![Immunoblots on membranes prepared from whole mouse (MBM) and rat (RBM) brain, and from human hippocampus [HBM(H)], cerebral cortex [HBM(Cx)], and cerebellum [HBM(Cb)].](http://www.antibodiesinc.com/cdn/shop/products/validation-image-73-168-n147-6-wb3_61988529-2c8a-4bc7-a54a-e0675f05b9f3_1024x.jpg?v=1626903796)
Product Specific References for Applications and Species
- HITS-CLIP: Human
- Immunocytochemistry: Human | Mouse
- Immunohistochemistry: Human | Mouse | Rat
- Western Blot: Human | Mouse
| HITS-CLIP: Human | ||
| PMID | Dilution | Publication |
| 38112323 | not listed | Neumann, DP, et al. 2024. The landscape of alternative polyadenylation during EMT and its regulation by the RNA-binding protein Quaking. RNA Biology, 45302. |
| Immunocytochemistry: Human | ||
| PMID | Dilution | Publication |
| 27454326 | 1:100 | Bin, J.M., et al. 2016. The oligodendrocyte-specific antibody ''CC1'' binds Quaking 7.. Journal of Neurochemistry, 181-186. |
| 26905650 | not listed | de Bruin, R.G., et al. 2016. The RNA-binding protein quaking maintains endothelial barrier function and affects VE-cadherin and β-catenin protein expression. Scientific Reports, 21643. |
| Immunocytochemistry: Mouse | ||
| PMID | Dilution | Publication |
| 30667360 | 1:5 (supe) | Andrews, N.P., et al. 2019. A toolbox of IgG subclass-switched recombinant monoclonal antibodies for enhanced multiplex immunolabeling of brain. Elife, e43322. |
| Immunohistochemistry: Human | ||
| PMID | Dilution | Publication |
| 27029405 | not listed | De Bruin, R.G., et al. 2016. Quaking promotes monocyte differentiation into pro-atherogenic macrophages by controlling pre-mRNA splicing and gene expression. Nature Communications, 10846. |
| 23963726 | not listed | van der Veer, E.P., et al. 2013. Quaking, an RNA-binding protein, is a critical regulator of vascular smooth muscle cell phenotype.. Circulation Research, 1065-1075. |
| Immunohistochemistry: Mouse | ||
| PMID | Dilution | Publication |
| 39308343 | 1:1000 | Min, K.W., et al. 2024. Mature microRNA-binding protein QKI suppresses extracellular microRNA let-7b release. Journal of Cell Science, . |
| 36336067 | 1:100 | New, L.E., et al. 2022. GABAergic Regulation Of Cell Proliferation Within The Adult Mouse Spinal Cord. Neuropharmacology, 109326. |
| 33378678 | 1:200 | Lee, J., et al. 2020. QUAKING regulates microexon alternative splicing of the rho GTPase pathway and controls microglia homeostasis.. Cell Reports, 108560. |
| 26038197 | 1:2 (supe) | Corns, L.F., et al. 2015. Cholinergic Enhancement of Cell Proliferation in the Postnatal Neurogenic Niche of the Mammalian Spinal Cord.. Stem Cells., 2864-76. |
| Immunohistochemistry: Rat | ||
| PMID | Dilution | Publication |
| 33227495 | 1:100 | Patel, B., et al. 2021. Inhibition of mitochondrial fission and iNOS in the dorsal vagal complex protects from overeating and weight gain. Molecular Metabolism, 101123. |
| 22675541 | not listed | Manning CF, et al. 2012. Benefits and pitfalls of secondary antibodies: why choosing the right secondary is of primary importance.. PLoS One, e38313. |
| Western Blot: Human | ||
| PMID | Dilution | Publication |
| 38112323 | 1:5000 | Neumann, DP, et al. 2024. The landscape of alternative polyadenylation during EMT and its regulation by the RNA-binding protein Quaking. RNA Biology, 45302. |
| 35544276 | not listed | Fagg, W.S., et al. 2022. Definition of germ layer cell lineage alternative splicing programs reveals a critical role for Quaking in specifying cardiac cell fate. Nucleic acids research, 5313-5334. |
| 34673031 | 1:1000 | Kundinger, S.R., et al. 2021. Phosphorylation regulates arginine-rich RNA-binding protein solubility and oligomerization. The Journal of biological chemistry, 101306. |
| 34428287 | not listed | Liao, K.C., et al. 2021. The RNA binding protein Quaking represses splicing of the Fibronectin EDA exon and downregulates the interferon response. Nucleic Acids Research, 10034-10045. |
| 31829086 | not listed | Liao, K.C., et al. 2020. The RNA binding protein Quaking represses host interferon response by downregulating MAVS. RNA Biology, 366-380. |
| 27454326 | 1:1000 | Bin, J.M., et al. 2016. The oligodendrocyte-specific antibody ''CC1'' binds Quaking 7.. Journal of Neurochemistry, 181-186. |
| 23630077 | 1:1000 | Teplova, M., et al. 2013. Structure-function studies of STAR family Quaking proteins bound to their in vivo RNA target sites.. Genes & Development, 928-940. |
| 21467216 | 1:1000 | Sánchez-Quiles, V., et al. 2010. HSV-1 Cgal+ infection promotes quaking RNA binding protein production and induces nuclear-cytoplasmic shuttling of quaking I-5 isoform in human hepatoma cells.. Molecular & Cellular Proteomics, . |
| Western Blot: Mouse | ||
| PMID | Dilution | Publication |
| 34968236 | not listed | de Bruin, R.G., et al. 2020. Targeting the RNA-Binding Protein QKI in Myeloid Cells Ameliorates Macrophage-Induced Renal Interstitial Fibrosis. Epigenomes, 2. |
| 30918328 | not listed | Azam, S.H., et al. 2019. Quaking orchestrates a post-transcriptional regulatory network of endothelial cell cycle progression critical to angiogenesis and metastasis. Oncogene, 5191-5210. |
| 30050412 | not listed | Shi, Q., et al. 2018. mTORC1 Activation by Loss of Tsc1 in Myelinating Glia Causes Downregulation of Quaking and Neurofascin 155 Leading to Paranodal Domain …. Frontiers in Cellular Neuroscience, 201. |
| 27317697 | not listed | Llorian, M., et al. 2016. The alternative splicing program of differentiated smooth muscle cells involves concerted non-productive splicing of post-transcriptional regulators.. Nucleic Acids Research, 8933-8950. |
| 20956316 | not listed | Zhao, L., et al. 2010. Quaking I controls a unique cytoplasmic pathway that regulates alternative splicing of myelin-associated glycoprotein.. PNAS: USA, 19061-19066. |