Anti-QKI-6 Antibody (N182/17)

Name: Anti-QKI-6 Antibody
Brand: NeuroMab
SKU: 75-190
Price: 319.0 USD
Availability: InStock

Our Anti-QKI-6 mouse monoclonal primary antibody from NeuroMab is produced in-house from hybridoma clone N182/17. It detects human and mouse QKI-6, and is purified by Protein A chromatography. It is great for use in IHC, ICC, WB.

Adult rat hippocampus immunohistochemistry.

Transfected cell immunoblot: extracts of COS cells transiently transfected with Flag-tagged QKI-5, QKI-6, QKI-7 and QKI-7b and untagged Kv2.1 plasmids and probed with N182/17 (left) and mouse anti-Flag (right.

Western blot of protein extracted from UD hESCs, DE cells, and CM cells probed with antibodies for QKI5 (magenta, top panel) and panQKI (green), QKI6 (magenta, middle panel) and panQKI (green), or QKI7 (magenta, bottom panel; ND denotes not detectable) and panQKI (green). Image from publication CC-BY-4.0. PMID: 35544276

Adult rat hippocampus immunohistochemistry.

Species Reactivity

Human, Mouse

Applications

ICC, IHC, WB

Host Species

Mouse

Available Conjugates

490

550

594

650

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Data Sheet

SKU: 75-190

Volume: 100 µL

Variant

Price:

$319.00

Quantity:

Product Details

Target

QKI-6

Target Description

QKI6 is a member of the Signal Transduction and Activation of RNA proteins (STAR proteins) family. QKI5, QKI6, QKI7 and QKI7b are alternatively spliced mRNAs from the QKI1 gene. QKI6 is a RNA-binding protein. QKI6 has roles in the regulation of splicing, export and stability of mRNA. It is involved in myelinization. It is a cytoplasmic isoform. Diseases associated with this gene include Angiocentric Glioma, 6Q Terminal Deletion Syndrome and Schizophrenia.

Format

Purified by Protein A chromatography

Concentration

1 mg/mL

Clonality

Monoclonal

Clone

N182/17

Isotype

IgG1

Applications

ICC, IHC, WB

Host Species

Mouse

Gene Name

Qki Qk QKI HKQ Qk1 Qka1

Molecular Weight

36-38 kDa

Antigen

Synthetic peptide amino acids 329-341 (PSGVLGMAFPTKG, C-terminal tail) of human QKI-6 (accession number Q96PU8-2)

Species Reactivity

Human, Mouse

Antibody Registry ID

AB_10673512

Storage

Aliquot and store at ≤ -20°C for long term storage. For short term storage, store at 2-8°C. For maximum recovery of product, centrifuge the vial prior to removing the cap.

Physical State

Liquid

Production Notes

Produced by in vitro bioreactor culture of hybridoma line followed by Protein A affinity chromatography. Purified mAbs are >90% specific antibody.

Buffer

10 mM Tris, 50 mM Sodium Chloride, 0.065% Sodium Azide pH 7.118

Conjugate

Unconjugated

Specificity

Does not cross-react with QKI-5, QKI-7 or QKI-7b

Quality Control

Each new lot of antibody is quality control tested by western blot on rat whole brain lysate and confirmed to stain the expected molecular weight band.

Usage Statement

These antibodies are to be used as research laboratory reagents and are not for use as diagnostic or therapeutic reagents in humans.

Country of Origin

United States

Expiration

24 months from date of receipt

Shipping

Shipped on ice packs

Synonyms

Protein quaking (Hqk) (HqkI)

UniProt Details

UniProt (Human): Q96PU8
UniProt (Immunogen Species): Q96PU8-2

Product Specific References for Applications and Species

Immunocytochemistry: Human
Immunohistochemistry: Human | Mouse
Western Blot: Human | Mouse

Immunocytochemistry: Human
PMID	Dilution	Publication
27454326	1:100	Bin, J.M., et al. 2016. The oligodendrocyte-specific antibody ''CC1'' binds Quaking 7.. Journal of Neurochemistry, 181-186.

Immunohistochemistry: Human
PMID	Dilution	Publication
27029405	10ug/ml	De Bruin, R.G., et al. 2016. Quaking promotes monocyte differentiation into pro-atherogenic macrophages by controlling pre-mRNA splicing and gene expression. Nature Communications, 10846.
23963726	not listed	van der Veer, E.P., et al. 2013. Quaking, an RNA-binding protein, is a critical regulator of vascular smooth muscle cell phenotype.. Circulation Research, 1065-1075.

Immunohistochemistry: Mouse
PMID	Dilution	Publication
32202512	not listed	Zhou, X., et al. 2020. Mature myelin maintenance requires Qki to coactivate PPARβ-RXRα-mediated lipid metabolism. Journal of Clinical Investigation, 2220-2236.
27196066	not listed	Suiko, T., et al. 2016. Expression of Quaking RNA-Binding Protein in the Adult and Developing Mouse Retina. PLoS One, e0156033.

Western Blot: Human
PMID	Dilution	Publication
35544276	not listed	Fagg, W.S., et al. 2022. Definition of germ layer cell lineage alternative splicing programs reveals a critical role for Quaking in specifying cardiac cell fate. Nucleic acids research, 5313-5334.
27454326	1:1000	Bin, J.M., et al. 2016. The oligodendrocyte-specific antibody ''CC1'' binds Quaking 7.. Journal of Neurochemistry, 181-186.
27029405	1:1000	De Bruin, R.G., et al. 2016. Quaking promotes monocyte differentiation into pro-atherogenic macrophages by controlling pre-mRNA splicing and gene expression. Nature Communications, 10846.

Western Blot: Mouse
PMID	Dilution	Publication
34968236	not listed	de Bruin, R.G., et al. 2020. Targeting the RNA-Binding Protein QKI in Myeloid Cells Ameliorates Macrophage-Induced Renal Interstitial Fibrosis. Epigenomes, 2.
31331967	1:1000	Caines, R., et al. 2019. The RNA-binding protein QKI controls alternative splicing in vascular cells, producing an effective model for therapy. Journal Cell Science, jcs230276.
24449917	not listed	Chan, C.B., et al. 2014. PIKE is essential for oligodendroglia development and CNS myelination.. PNAS USA, 1993-1998.

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Anti-QKI-6 Antibody (N182/17)

SKU: 75-190

Product Details

Product Specific References for Applications and Species

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