Our Anti-QKI-7 mouse monoclonal primary antibody from NeuroMab is produced in-house from hybridoma clone N183/15. It detects human and mouse QKI-7, and is purified by Protein A chromatography. It is great for use in IHC, ICC, WB.
QKI7 is a member of the Signal Transduction and Activation of RNA proteins (STAR proteins) family. QKI5, QKI6, QKI7 and QKI7b are alternatively spliced mRNAs from the QKI1 gene. QKI7 is a RNA-binding protein. QKI6 has roles in the regulation of splicing, export and stability of mRNA. It is involved in myelinization. It is a cytoplasmic isoform. Diseases associated with this gene include Angiocentric Glioma, 6Q Terminal Deletion Syndrome and Schizophrenia.
Purified by Protein A chromatography
1 mg/mL
Monoclonal
N183/15
IgG1
ICC, IHC, WB
Mouse
Qki Qk QKI HKQ Qk1 Qka1
36-38 kDa
Synthetic peptide amino acids 303-317 (LEWIEMPVMPDISAH, C-terminal tail) of human QKI-7 (accession number Q96PU8-5)
Human, Mouse
AB_2173148
Aliquot and store at ≤ -20°C for long term storage. For short term storage, store at 2-8°C. For maximum recovery of product, centrifuge the vial prior to removing the cap.
Liquid
Produced by in vitro bioreactor culture of hybridoma line followed by Protein A affinity chromatography. Purified mAbs are >90% specific antibody.
10 mM Tris, 50 mM Sodium Chloride, 0.065% Sodium Azide pH 7.125
WB: 1:1000
IHC: 1:200
ICC: 1:200
Unconjugated
Does not cross-react with QKI-5, QKI-6 or QKI-7b
Each new lot of antibody is quality control tested by western blot on rat whole brain lysate and confirmed to stain the expected molecular weight band.
These antibodies are to be used as research laboratory reagents and are not for use as diagnostic or therapeutic reagents in humans.
de Bruin, R.G., et al. 2014. Quaking promotes monocyte differentiation into pro-atherogenic macrophages by controlling pre-mRNA splicing and gene expression.. Nature Communications, 10846.
van der Veer, E.P., et al. 2013. Quaking, an RNA-binding protein, is a critical regulator of vascular smooth muscle cell phenotype.. Circulation Research, 1065-1075.
De Nuccio, F., et al. 2023. Oligodendrocytes Prune Axons Containing α-Synuclein Aggregates In Vivo: Lewy Neurites as Precursors of Glial Cytoplasmic Inclusions in Multiple System Atrophy?. Biomolecules, 269.
Fagg, W.S., et al. 2022. Definition of germ layer cell lineage alternative splicing programs reveals a critical role for Quaking in specifying cardiac cell fate. Nucleic acids research, 5313-5334.
