Anti-RBM17/SPF45 Antibody (N219/5)

Name: Anti-RBM17/SPF45 Antibody
Brand: NeuroMab
SKU: 75-234
Price: 319.0 USD
Availability: InStock

Our Anti-RBM17/SPF45 mouse monoclonal primary antibody from NeuroMab is produced in-house from hybridoma clone N219/5. It detects human, mouse, and rat RBM17/SPF45, and is purified by Protein A chromatography. It is great for use in IHC, ICC, WB.

Adult rat hippocampus immunohistochemistry.

Species Reactivity

Human, Mouse, Rat

Applications

ICC, IHC, WB

Host Species

Mouse

Available Conjugates

490

550

594

650

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Data Sheet

SKU: 75-234

Volume: 100 µL

Variant

Price:

$319.00

Quantity:

Product Details

Target

RBM17/SPF45

Target Description

RNA-Binding Motif Protein 17 or Splicing factor 45 is encoded by the gene RBM17. RBM17 is a component of the spliceosome complex and displays catalytic activity during mRNA splicing. RBM17 is involved in alternative splicing, resulting in different isoforms of a gene. RBM is also thought to play a role in DNA repair (Red: Chaouki et al 2006) RBM17 is widely expressed in the nucleus and important for development. Diseases associated with this gene include sickle cell anemia and forms of Ataxia 1.

Format

Purified by Protein A chromatography

Concentration

1 mg/mL

Clonality

Monoclonal

Clone

N219/5

Isotype

IgG1

Applications

ICC, IHC, WB

Host Species

Mouse

Gene Name

RBM17 SPF45

Molecular Weight

45-52 kDa

Antigen

Fusion protein amino acids 100-200 of human RBM17 (accession number Q96I25) produced recombinantly in E. Coli

Species Reactivity

Human, Mouse, Rat

Antibody Registry ID

AB_10675301

Storage

Aliquot and store at ≤ -20°C for long term storage. For short term storage, store at 2-8°C. For maximum recovery of product, centrifuge the vial prior to removing the cap.

Physical State

Liquid

Production Notes

Produced by in vitro bioreactor culture of hybridoma line followed by Protein A affinity chromatography. Purified mAbs are >90% specific antibody.

Buffer

10 mM Tris, 50 mM Sodium Chloride, 0.065% Sodium Azide pH 7.125

Conjugate

Unconjugated

Specificity

No cross-reactivity reported

Quality Control

Each new lot of antibody is quality control tested by western blot on rat whole brain lysate and confirmed to stain the expected molecular weight band.

Usage Statement

These antibodies are to be used as research laboratory reagents and are not for use as diagnostic or therapeutic reagents in humans.

Country of Origin

United States

Expiration

24 months from date of receipt

Shipping

Shipped on ice packs

Synonyms

Splicing factor 45 (45 kDa-splicing factor) (RNA-binding motif protein 17)

UniProt Details

UniProt (Human): Q96I25
UniProt (Immunogen Species): Q96I25

Product Specific References for Applications and Species

Immunohistochemistry: Mouse
Western Blot: Mouse

Immunohistochemistry: Mouse
PMID	Dilution	Publication
28007900	not listed	Tan, Q., et al. 2016. Extensive cryptic splicing upon loss of RBM17 and TDP43 in neurodegeneration models. Human Molecular Genetics, 5083-5093.

Western Blot: Mouse
PMID	Dilution	Publication
30332651	1:2500	De Maio, A., et al. 2018. RBM17 Interacts with U2SURP and CHERP to Regulate Expression and Splicing of RNA-Processing Proteins. Cell Reports, 726-736.

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Anti-RBM17/SPF45 Antibody (N219/5)

SKU: 75-234

Product Details

Product Specific References for Applications and Species

Bulk Order Anti-RBM17/SPF45 Antibody

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