Anti-SAP97 Antibody (K64/15)
Our Anti-SAP97 mouse monoclonal primary antibody from NeuroMab is produced in-house from hybridoma clone K64/15. It is KO validated, detects human, mouse, and rat SAP97, and is purified by Protein A chromatography. It is great for use in IHC, ICC, IP, WB.
![Immunoblots on brain membranes prepares from whole rat (RBM) and mouse (MBM) brain, and from human cerebral cortex [HBM(Cx)], and hippocampus [HBM(H)]](http://www.antibodiesinc.com/cdn/shop/products/validation-image-73-030-k64-15-wb-3_{width}x.jpg?v=1626902846)
Adult rat hippocampus immunohistochemistry
![Immunoblots on brain membranes prepares from whole rat (RBM) and mouse (MBM) brain, and from human cerebral cortex [HBM(Cx)], and hippocampus [HBM(H)]](http://www.antibodiesinc.com/cdn/shop/products/validation-image-73-030-k64-15-wb-3_1024x.jpg?v=1626902846)
Product Specific References for Applications and Species
- CoIP: Mouse
- ELISA: Mouse
- Immunocytochemistry: Rat
- Immunohistochemistry: Mouse | Rabbit
- Western Blot: Mouse | Rat
- Additional Publications: Unspecified
| Co-Immunoprecipitation: Mouse | ||
| PMID | Dilution | Publication |
| 25673833 | 10ug | Waters, E.M., et al. 2015. G-protein-coupled estrogen receptor 1 is anatomically positioned to modulate synaptic plasticity in the mouse hippocampus.. Journal of Neuroscience, 2384-2397. |
| ELISA: Mouse | ||
| PMID | Dilution | Publication |
| 22855505 | not listed | Liu, W., et al. 2012. The scaffolding protein synapse-associated protein 97 is required for enhanced signaling through isotype-switched IgG memory B cell receptors.. Science Signaling, ra54. |
| Immunocytochemistry: Rat | ||
| PMID | Dilution | Publication |
| 20100836 | not listed | Marcello, E., et al. 2010. An arginine stretch limits ADAM10 exit from the endoplasmic reticulum.. The Journal of Biological Chemistry, 10376-10384. |
| Immunohistochemistry: Mouse | ||
| PMID | Dilution | Publication |
| 34393723 | 1:250 | Vila A, et al. 2021. Synaptic Scaffolds, Ion Channels and Polyamines in Mouse Photoreceptor Synapses: Anatomy of a Signaling Complex. Frontiers in Cellular Neuroscience, 667046. |
| Immunohistochemistry: Rabbit | ||
| PMID | Dilution | Publication |
| 27558197 | 1:500 | Vila, A., et al. 2015. Membrane-associated guanylate kinase scaffolds organize a horizontal cell synaptic complex restricted to invaginating contacts with photoreceptors.. Journal of Comparative Neurology, 850-867. |
| Western Blot: Mouse | ||
| PMID | Dilution | Publication |
| 34613779 | not listed | Ban, Y., et al. 2021. Prickle promotes the formation and maintenance of glutamatergic synapses by stabilizing the intercellular planar cell polarity complex. Science Advances, eabh2974. |
| 32205379 | 1:1000 | Cheng, W., et al. 2020. Proteasomal-Mediated Degradation of AKAP150 Accompanies AMPAR Endocytosis during cLTD. eNeuro, . |
| 31575955 | 1:500 | Freudneberg, F., et al. 2019. Quantitative analysis of Gria1, Gria2, Dlg1 and Dlg4 expression levels in hippocampus following forced swim stress in mice. Scientific Reports, 14060. |
| 31169885 | not listed | Dong, X., et al. 2019. Discs large homolog 1 regulates B-cell proliferation and antibody production. International Immunology, 759-770. |
| 31028120 | not listed | Dong, X., et al. 2019. Dlg1 maintains dendritic cell function by securing voltage-gated K+ channel integrity. . The Journal of Immunology, 3187-3197. |
| 30586380 | not listed | Favaro PD, et al. 2018. An opposing function of paralogs in balancing developmental synapse maturation. Plos Biology, e2006838. |
| 28291226 | 1:50 | Fragkouli, A., et al. 2017. Neuronal ELAVL proteins utilize AUF-1 as a co-partner to induce neuron-specific alternative splicing of APP.. Scientific Reports, 44507. |
| 27535911 | 1:1000 | Sim, S.E., et al. 2016. The Brain-Enriched MicroRNA miR-9-3p Regulates Synaptic Plasticity and Memory. Journal of Neuroscience, 8641-8652. |
| 26085624 | 1:2000 | Tindi JO, et al. 2015. ANKS1B Gene Product AIDA-1 Controls Hippocampal Synaptic Transmission by Regulating GluN2B Subunit Localization.. Journal of Neuroscience, 8986-96. |
| 25505322 | not listed | Fortin, D.A., et al. 2014. Live imaging of endogenous PSD-95 using ENABLED: a conditional strategy to fluorescently label endogenous proteins. Journal of Neuroscience, 16698-16712. |
| 24795561 | 1:1000 | Joaquin N. Lugo, et al. 2014. Deletion of PTEN produces autism-like behavioral deficits and alterations in synaptic proteins.. Frontiers in Molecular Neuroscience, 27. |
| 24687281 | 1:250 | Heller, B.A., et al. 2014. Functionally distinct PI 3-kinase pathways regulate myelination in the peripheral nervous system.. The Journal of Biology, 1219-1236. |
| 23946397 | not listed | Bonnet, S.A., et al. 2013. Synaptic state-dependent functional interplay between postsynaptic density-95 and synapse-associated protein 102.. Journal of Neuroscience, 13398-13409. |
| 23719805 | 1:1000 | Hoy, J.L., et al. 2013. Neuroligin1 drives synaptic and behavioral maturation through intracellular interactions.. Journal of Neuroscience, 9364-9384. |
| 23297362 | not listed | Ng, A.A., et al. 2013. The CMT4B disease-causing phosphatases Mtmr2 and Mtmr13 localize to the Schwann cell cytoplasm and endomembrane compartments, where they depend upon each other to achieve wild-type levels of protein expression.. Human Molecular Genetics, 1493-1506. |
| 23268962 | 1:200 | Nagura, H., et al. 2012. Impaired synaptic clustering of postsynaptic density proteins and altered signal transmission in hippocampal neurons, and disrupted learning behavior in PDZ1 and PDZ2 ligand binding-deficient PSD-95 knockin mice.. Molecular Brain, 43. |
| 20547132 | not listed | Sumioka, A., et al. 2010. TARP phosphorylation regulates synaptic AMPA receptors through lipid bilayers.. Neuron, 755-767. |
| Western Blot: Rat | ||
| PMID | Dilution | Publication |
| 37079350 | not listed | Bonnet, C., et al. 2023. Regulation of different phases of AMPA receptor intracellular transport by 4.1N and SAP97. Elife, e85609. |
| 35145085 | 1:500 | Kay, Y., et al. 2022. Schizophrenia-associated SAP97 mutations increase glutamatergic synapse strength in the dentate gyrus and impair contextual episodic memory in rats. . Nature communications, 45307. |
| 34592633 | 1:3000 | Del Angel, Y.C., et al. 2021. Down-regulation of AMPA receptors and long-term potentiation during early epileptogenesis. Epilepsy Behavior, 108320. |
| 29218323 | not listed | Zhang, L., et al. 2017. SAP97 Binding Partner CRIPT Promotes Dendrite Growth In Vitro and In Vivo. eNeuro, . |
| 28152104 | 1:2000 | von Ossowski, L., et al. 2017. Cysteine 893 is a target of regulatory thiol modifications of GluA1 AMPA receptors. Plos One, e0171489. |
| 27026592 | not listed | Boccitto, M., et al. 2016. Opposing actions of the synapse-associated protein of 97-kDa molecular weight (SAP97) and Disrupted in Schizophrenia 1 (DISC1) on Wnt/β-catenin signaling. Neuroscience, 22-30. |
| 25701814 | not listed | Zhang, L., et al. 2015. Structure-function analysis of SAP97, a modular scaffolding protein that drives dendrite growth.. Molecular and Cellular Neuroscience, 31-44. |
| 24705401 | 1:1000 | Bustos, F.J., et al. 2014. PSD95 suppresses dendritic arbor development in mature hippocampal neurons by occluding the clustering of NR2B-NMDA receptors.. PLoS One, e94037. |
| 24687281 | 1:250 | Heller, B.A., et al. 2014. Functionally distinct PI 3-kinase pathways regulate myelination in the peripheral nervous system.. The Journal of Biology, 1219-1236. |
| 21911612 | 1:100 | Joseph, B.K., et al. 2011. Postsynaptic density-95 scaffolding of Shaker-type K⁺ channels in smooth muscle cells regulates the diameter of cerebral arteries.. The Journal of Physiology, 5143-5152. |
| 19858198 | 1:1000 | Nikandrova, Y.A., et al. 2010. Ca2+/calmodulin-dependent protein kinase II binds to and phosphorylates a specific SAP97 splice variant to disrupt association with AKAP79/150 and modulate alpha-amino-3-hydroxy-5-methyl-4-isoxazolepropionic acid-type glutamate receptor (AMPAR) activity.. The Journal of Biological Chemistry, 923-934. |
| 17670980 | not listed | Al-Hallaq, R.A., et al. 2007. NMDA di-heteromeric receptor populations and associated proteins in rat hippocampus.. Journal of Neuroscience, 8334-8343. |
| Additional Publications: Unspecified | ||
| PMID | Publication | |
| 31923461 | Loomis, C., et al. 2020. Identification of MAGUK scaffold proteins as intracellular binding partners of synaptic adhesion protein Slitrk2. Molecular and Cellular Neuroscience, 103465. | |
| 30836092 | Bach, A., et al. 2019. Selectivity, efficacy and toxicity studies of UCCB01-144, a dimeric neuroprotective PSD-95 inhibitor. Neuropharmacology, 100-111. | |
| 29212815 | Purlyte, E., et al. 2018. Rab29 activation of the Parkinson's disease‐associated LRRK2 kinase.. The EMBO Journal, 45309. | |
| 27796754 | Caffino, L., et al. 2017. Ketamine Self-Administration Reduces the Homeostasis of the Glutamate Synapse in the Rat Brain. Molecular Neurobiology, 7186-7193. | |
| 26684546 | Nagura, H., et al. 2016. Characterization of physiological phenotypes of dentate gyrus synapses of PDZ1/2 domain-deficient PSD-95-knockin mice.. European Journal of Neuroscience, 618-625. | |
| 25365955 | Jafari, Z., et al. 2015. Bilingual proficiency and cognitive reserve in Persian-English bilingual older adults.. Aging Clinical and Experimental Research, 351-357. | |
| 24911070 | Caffino, L., et al. 2014. Cocaine-induced glutamate receptor trafficking is abrogated by extinction training in the rat hippocampus.. Pharmacological Reports, 198-204. | |