Our Anti-Slo1/BKAlpha potassium channel mouse monoclonal primary antibody from NeuroMab is produced in-house from hybridoma clone L6/23. It is KO validated, detects mouse and rat Slo1/BKAlpha potassium channel, and is purified by Protein A chromatography. It is great for use in IHC, ICC, IP, WB.
Immunoblot against crude membranes from adult rat brain (RBM), wild-type (MBM-WT) and BKAlpha knockout (MBM- BKaKO) mouse brains probed with L6/23 (left), L6/60 (middle) or N52A/42 (right) TC supe. Reproduced from PLoS One (2010 Wersinger et al, PMID 21079807).
Calcium-activated potassium channel subunit alpha-1, Potassium Calcium-Activated Channel Subfamily M Alpha 1 or Slo1/BKalpha is encoded by the gene KCNMA1. BKalpha binds to one of 4 different beta subunits to form a channel in the calcium activated large conductance (MaxiK channel) family. This potassium channel is activated by membrane depolarization or an increase in cytosolic ca2+ and mediated the export of K+. BKalpha is involved with various cell processes including contraction of smooth muscle, regulation of transmitter release, innate immunity and regulation of membrane potential. Diseases associated with KCNMA1 include forms of Epilepsy and Developmental Delay Cerebellar Atrophy and Seizures.
Purified by Protein A chromatography
1 mg/mL
Monoclonal
L6/23
IgG1
ICC, IHC, IP, WB
Mouse
Kcnma1 Kcnma
110-130 kDa
Fusion protein amino acids 690-1196 (cytoplasmic C-terminus) of mouse Slo1 (accession number Q08460) produced recombinantly in E. Coli
Mouse, Rat
AB_2491105
Aliquot and store at ≤ -20°C for long term storage. For short term storage, store at 2-8°C. For maximum recovery of product, centrifuge the vial prior to removing the cap.
Liquid
Produced by in vitro bioreactor culture of hybridoma line followed by Protein A affinity chromatography. Purified mAbs are >90% specific antibody.
10 mM Tris, 50 mM Sodium Chloride, 0.065% Sodium Azide pH 7.125
IHC: 1:250
Unconjugated
No cross-reactivity reported
Each new lot of antibody is quality control tested by western blot on rat whole brain lysate and confirmed to stain the expected molecular weight band.
These antibodies are to be used as research laboratory reagents and are not for use as diagnostic or therapeutic reagents in humans.
Jeng, J.Y., et al. 2022. AAV-Mediated Rescue of Eps8 Expression In Vivo Restores Hair-Cell Function in a Mouse Model of Recessive Deafness. Molecular Therapy Methods & Clinical Development, 355-370.
Carlton, A., et al. 2020. Loss of Baiap2l2 destabilizes the transducing stereocilia of cochlear hair cells and leads to deafness. The Journal of Physiology, 1173-1198.
Zorrilla de San Martín, J., et al. 2010. Ca(2+) and Ca(2+)-activated K(+) channels that support and modulate transmitter release at the olivocochlear efferent-inner hair cell synapse.. Journal of Neuroscience, 12157-12167.
