Our Anti-TRIP8b (constant) mouse monoclonal primary antibody from NeuroMab is produced in-house from hybridoma clone N212/17. It is KO validated, detects human, mouse, and rat TRIP8b (constant), and is purified by Protein A chromatography. It is great for use in IHC, ICC, WB.
Immunoblot versus crude membranes from adult rat brain (RBM) and WT and TRIP8b KO mouse brains probed with N212/17 (left) and N52A/42 (right) TC supe. Mouse samples courtesy of Dane Chetkovich (Northwestern University).
PEX5-related protein, Peroxisomal Biogenesis Factor 5 Like, TPR-containing Rab8b-interacting protein or TRIP8b is encoded by the gene Pex5l. It is a member of the peroxisomal targeting signal receptor family. TRIP8b is a cytoplasmic accessory subunit of hyperpolarization-activated cyclic nucleotide-gated (HCN) channels. TRIP8b interacts with the carboxyl-terminal region of HCN channels and regulates their cell-surface expression to the dendrites in many types of neurons. TRIP8b plays a role in regulation of HCN channel cyclic nucleotide kinetics. TRIP8b is expressed in the brain. Diseases associated with PEX5L include Rhizomelic Chondrodysplasia Punctata, Type 5 and Rhizomelic Chondrodysplasia Punctata, Type 2.
Purified by Protein A chromatography
1 mg/mL
Monoclonal
N212/17
IgG2a
ICC, IHC, IP, WB
Mouse
Pex5l Pex2 Pex5r Trip8b
70 kDa
Fusion protein amino acids 1-192 (exon 1a, exon 4 and constant region) of rat TRIP8b (accession number Q925N3) produced recombinantly in E. Coli
Human, Mouse, Rat
AB_10698035
Aliquot and store at ≤ -20°C for long term storage. For short term storage, store at 2-8°C. For maximum recovery of product, centrifuge the vial prior to removing the cap.
Liquid
Produced by in vitro bioreactor culture of hybridoma line followed by Protein A affinity chromatography. Purified mAbs are >90% specific antibody.
10 mM Tris, 50 mM Sodium Chloride, 0.065% Sodium Azide pH 7.125
WB: 1:1000
IHC: 1:500
ICC: 1:500
Unconjugated
Cross-reacts with multiple TRIP8b isoforms
Each new lot of antibody is quality control tested by western blot on rat whole brain lysate and confirmed to stain the expected molecular weight band.
These antibodies are to be used as research laboratory reagents and are not for use as diagnostic or therapeutic reagents in humans.
Otto M, et al. 2020. 12(S)-HETE mediates diabetes-induced endothelial dysfunction by activating intracellular endothelial cell TRPV1. Journal of Clinical Investigation, 4999-5010.
Lyman, KA, et al. 2024. Characterization of hyperpolarization-activated cyclic nucleotide-gated channels in oligodendrocytes. Frontiers in Cellular Neuroscience, 1321682.
Chaudhary, R, et al. 2022. Modulation of Pacemaker Channel Function in a Model of Thalamocortical Hyperexcitability by Demyelination and Cytokines. Cerebral Cortex, 4397-4421.
Salling MC, et al. 2018. Alcohol Consumption during Adolescence in a Mouse Model of Binge Drinking Alters the Intrinsic Excitability and Function of the Prefrontal Cortex through a Reduction in the Hyperpolarization-Activated Cation Current. Journal of Neuroscience, 6207-6222.
Zobeiri M, et al. 2018. Modulation of thalamocortical oscillations by TRIP8b, an auxiliary subunit for HCN channels. Brain Structure and Function, 1537-1564.
Piskorowski R, et al. 2011. TRIP8b splice forms act in concert to regulate the localization and expression of HCN1 channels in CA1 pyramidal neurons.. Neuron, 495-509.
Lyman KA, et al. 2017. Allostery between two binding sites in the ion channel subunit TRIP8b confers binding specificity to HCN channels. Journal of Biological Chemistry, 17718-07730.
Hughes BA, et al. 2020. Chronic ethanol exposure alters prelimbic prefrontal cortical Fast-Spiking and Martinotti interneuron function with differential sex specificity in rat brain. Neuropharmacology, 107805.
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