Anti-TRIP8b (Constant) Antibody (N212/7)

Name: Anti-TRIP8b (Constant) Antibody
Brand: NeuroMab
SKU: 75-243
Price: 319.0 USD
Availability: InStock

4 Citations

Our Anti-TRIP8b (constant) mouse monoclonal primary antibody from NeuroMab is produced in-house from hybridoma clone N212/7. It is KO validated, detects human, mouse, and rat TRIP8b (constant), and is purified by Protein A chromatography. It is great for use in IHC, ICC, WB.

Adult rat hippocampus immunohistochemistry.

Transfected cell immunoblot: extracts of COS cells transiently transfected with GFP-tagged TRIP8b-1a-4, TRIP8b-1b-2, TRIP8b-1a-2 or untagged Kv2.1 plasmid and probed with N212/7 (left) or N86/8 (right) TC supe.

Immunoblot versus crude membranes from adult rat brain (RBM) and WT and TRIP8b KO mouse brains probed with N212/7 (left) and N52A/42 (right) TC supe. Mouse brains courtesy of Dane Chetkovich (Northwestern University).

Adult rat hippocampus immunohistochemistry.

Species Reactivity

Human, Mouse, Rat

Applications

ICC, IHC, WB

Host Species

Mouse

Available Conjugates

490

550

594

650

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Data Sheet

KO Validated

SKU: 75-243

Volume: 100 µL

Variant

Price:

$319.00

Quantity:

Product Details

Target

TRIP8b (constant)

Target Description

PEX5-related protein, Peroxisomal Biogenesis Factor 5 Like, TPR-containing Rab8b-interacting protein or TRIP8b is encoded by the gene Pex5l. It is a member of the peroxisomal targeting signal receptor family. TRIP8b is a cytoplasmic accessory subunit of hyperpolarization-activated cyclic nucleotide-gated (HCN) channels. TRIP8b interacts with the carboxyl-terminal region of HCN channels and regulates their cell-surface expression to the dendrites in many types of neurons. TRIP8b plays a role in regulation of HCN channel cyclic nucleotide kinetics. TRIP8b is expressed in the brain. Diseases associated with PEX5L include Rhizomelic Chondrodysplasia Punctata, Type 5 and Rhizomelic Chondrodysplasia Punctata, Type 2.

Format

Purified by Protein A chromatography

Concentration

1 mg/mL

Clonality

Monoclonal

Clone

N212/7

Isotype

IgG2b

Applications

ICC, IHC, WB

Host Species

Mouse

Gene Name

Pex5l Pex2 Pex5r Trip8b

Molecular Weight

70 kDa

Antigen

Fusion protein amino acids 1-192 (exon 1a, exon 4 and constant region) of rat TRIP8b (accession number Q925N3) produced recombinantly in E. Coli

Species Reactivity

Human, Mouse, Rat

Antibody Registry ID

AB_10675329

Storage

Aliquot and store at ≤ -20°C for long term storage. For short term storage, store at 2-8°C. For maximum recovery of product, centrifuge the vial prior to removing the cap.

Physical State

Liquid

Production Notes

Produced by in vitro bioreactor culture of hybridoma line followed by Protein A affinity chromatography. Purified mAbs are >90% specific antibody.

Buffer

10 mM Tris, 50 mM Sodium Chloride, 0.065% Sodium Azide pH 7.125

Conjugate

Unconjugated

Specificity

Cross-reacts with multiple TRIP8b isoforms

Quality Control

Each new lot of antibody is quality control tested by western blot on rat whole brain lysate and confirmed to stain the expected molecular weight band.

Usage Statement

These antibodies are to be used as research laboratory reagents and are not for use as diagnostic or therapeutic reagents in humans.

Country of Origin

United States

Expiration

24 months from date of receipt

Shipping

Shipped on ice packs

Synonyms

PEX5-related protein (PEX5-like protein) (Peroxin-5-related protein) (TPR-containing Rab8b-interacting protein) (Tetratricopeptide repeat-containing Rab8b-interacting protein) (Pex5Rp) (TRIP8b)

UniProt Details

UniProt (Human): Q8IYB4
UniProt (Immunogen Species): Q925N3

Product Specific References for Applications and Species

Co-Immunoprecipitation: Mouse
Immunohistochemistry: Mouse
Western Blot: Mouse | Rat

Co-Immunoprecipitation: Mouse
PMID	Dilution	Publication
31045576	not listed	Hammelmann, V., et al. . Abolishing cAMP sensitivity in HCN2 pacemaker channels induces generalized seizures. JCI Insight, e126418.

Immunohistochemistry: Mouse
PMID	Dilution	Publication
35076711	1:50	Chaudhary, R., et al. 2022. Modulation of pacemaker channel function in a model of thalamocortical hyperexcitability by demyelination and cytokines. Cerebral Cortex, 4397-4421.

Western Blot: Mouse
PMID	Dilution	Publication
31045576	1:1000	Hammelmann, V., et al. 2019. Abolishing cAMP sensitivity in HCN2 pacemaker channels induces generalized seizures. JCI Insight, e126418.
23382386	not listed	Cao-Ehlker, X., et al. 2013. Up-regulation of hyperpolarization-activated cyclic nucleotide-gated channel 3 (HCN3) by specific interaction with K+ channel tetramerization domain-containing protein 3 (KCTD3).. Journal of Biological Chemistry, 7580-7589.

Western Blot: Rat
PMID	Dilution	Publication
29436607	1:800	Liu, Q., et al. 2018. Decreased hyperpolarization-activated cyclic nucleotide-gated channels are involved in bladder dysfunction associated with spinal cord injury. International Journal of Molecular Medicine, 2609-2618.

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Anti-TRIP8b (Constant) Antibody (N212/7)

SKU: 75-243

Product Details

Product Specific References for Applications and Species

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