Our Anti-TRIP8b (exon 4) mouse monoclonal primary antibody from NeuroMab is produced in-house from hybridoma clone N212/3. It is KO validated, detects human, mouse, and rat TRIP8b (exon 4), and is purified by Protein A chromatography. It is great for use in IHC, ICC, WB.
Transfected cell immunoblot: extracts of COS cells transiently transfected with GFP-tagged TRIP8b-1a-4, TRIP8b-1b-2, TRIP8b-1a-2 or untagged Kv2.1 plasmid and probed with N212/3 (left) and N86/8 (right) TC supe.
PEX5-related protein, Peroxisomal Biogenesis Factor 5 Like, TPR-containing Rab8b-interacting protein or TRIP8b is encoded by the gene Pex5l. It is a member of the peroxisomal targeting signal receptor family. TRIP8b is a cytoplasmic accessory subunit of hyperpolarization-activated cyclic nucleotide-gated (HCN) channels. TRIP8b interacts with the carboxyl-terminal region of HCN channels and regulates their cell-surface expression to the dendrites in many types of neurons. TRIP8b plays a role in regulation of HCN channel cyclic nucleotide kinetics. TRIP8b is expressed in the brain. Diseases associated with PEX5L include Rhizomelic Chondrodysplasia Punctata, Type 5 and Rhizomelic Chondrodysplasia Punctata, Type 2.
Purified by Protein A chromatography
1 mg/mL
Monoclonal
N212/3
IgG1
ICC, IHC, WB
Mouse
Pex5l Pex2 Pex5r Trip8b
70 kDa
Fusion protein amino acids 1-192 (exon 1a, exon 4 and constant region) of rat TRIP8b (accession number Q925N3) produced recombinantly in E. Coli
Human, Mouse, Rat
AB_10671951
Aliquot and store at ≤ -20°C for long term storage. For short term storage, store at 2-8°C. For maximum recovery of product, centrifuge the vial prior to removing the cap.
Liquid
Produced by in vitro bioreactor culture of hybridoma line followed by Protein A affinity chromatography. Purified mAbs are >90% specific antibody.
10 mM Tris, 50 mM Sodium Chloride, 0.065% Sodium Azide pH 7.125
Unconjugated
Specific for exon 4(does not react with TRIP8b isoforms containing exons 1a, 1b or 2)
Each new lot of antibody is quality control tested by western blot on rat whole brain lysate and confirmed to stain the expected molecular weight band.
These antibodies are to be used as research laboratory reagents and are not for use as diagnostic or therapeutic reagents in humans.
Brandalise, F., et al. 2020. Fragile X Mental Retardation Protein Bidirectionally Controls Dendritic Ih in a Cell Type-Specific Manner between Mouse Hippocampus and Prefrontal Cortex. Journal of Neuroscience, 5327-5340.
Rátkai, A., et al. 2021. Homeostatic plasticity and burst activity are mediated by hyperpolarization-activated cation currents and T-type calcium channels in neuronal cultures. Scientific Reports, 3236.
Foote, K.M., et al. 2019. Phosphorylation of the HCN channel auxiliary subunit TRIP8b is altered in an animal model of temporal lobe epilepsy and modulates channel function. Journal of Biological Chemistry, 15743-15758.
Piskorowski, R., et al. 2011. TRIP8b splice forms act in concert to regulate the localization and expression of HCN1 channels in CA1 pyramidal neurons.. Neuron, 495-509.
Luo, X.D., et al. 2023. Activation of Metabotropic Glutamate Receptor 1 Regulates Hippocampal CA1 Region Excitability in Rats with Status Epilepticus by Suppressing the HCN1 Channel. Neural Regeneration Research, 594-602.
Foote, K.M., et al. 2019. Phosphorylation of the HCN channel auxiliary subunit TRIP8b is altered in an animal model of temporal lobe epilepsy and modulates channel function. Journal of Biological Chemistry, 15743-15758.
Villarroel, H.S., et al. 2018. NPY Induces Stress Resilience via Downregulation of Ih in Principal Neurons of Rat Basolateral Amygdala. Journal of Neuroscience, 4505-4520.
Zhou, M., et al. 2015. Imbalance of HCN1 and HCN2 expression in hippocampal CA1 area impairs spatial learning and memory in rats with chronic morphine exposure.. Progress in Neuro-Psychopharmacology and Biological Psychiatry, 207-214.
Li, C.J., et al. 2014. Activation of GABAB receptors ameliorates cognitive impairment via restoring the balance of HCN1/HCN2 surface expression in the hippocampal CA1 area in rats with chronic cerebral hypoperfusion.. Molecular Neurobiology, 704-720.
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