Anti-VAPA/B Antibody (N479/107)
Our Anti-VAPA/B mouse monoclonal primary antibody from NeuroMab is produced in-house from hybridoma clone N479/107. It detects mouse and rat VAPA/B, and is purified by Protein A chromatography. It is great for use in IHC, ICC, WB.
SKU: 75-496
Product Details
VAPA/B
VAPA and VAPB are members of the VAMP-associated protein family (VAP) (aka vesicle-associated membrane proteins) which are abundant scaffolding proteins predominantly localized to the endoplasmic reticulum (ER). VAPs have recently been shown to interact with Kv2.1 and Kv2.2 channels forming junctions to link the ER to the plasma membrane (Johnson B. et al., 2018).
Purified by Protein A chromatography
1 mg/mL
Monoclonal
N479/107
IgG2b
ICC, IHC, WB
Mouse
Vapa Vap33
30 kDa
Fusion protein amino acids 1-219 (N-terminus) of rat VAPA (accession number Q9Z270) produced recombinantly in E. Coli
Mouse, Rat
AB_2722711
Aliquot and store at ≤ -20°C for long term storage. For short term storage, store at 2-8°C. For maximum recovery of product, centrifuge the vial prior to removing the cap.
Liquid
Produced by in vitro bioreactor culture of hybridoma line followed by Protein A affinity chromatography. Purified mAbs are >90% specific antibody.
10 mM Tris, 50 mM Sodium Chloride, 0.065% Sodium Azide pH 7.125
IHC: 1:250
Unconjugated
No cross-reactivity reported
Each new lot of antibody is quality control tested on cells overexpressing target protein and confirmed to give the expected staining pattern.
These antibodies are to be used as research laboratory reagents and are not for use as diagnostic or therapeutic reagents in humans.
United States
24 months from date of receipt
Shipped on ice packs
VAPA (Vesicle-associated membrane protein-associated protein A) (VAMP-associated protein A) (33 kDa VAMPassociatedprotein) (VAMP-A) (VAP-33)
UniProt (Human): Q9P0L0
UniProt (Immunogen Species): Q9Z270
UniProt (Immunogen Species): Q9Z270
Product Specific References for Applications and Species
Immunocytochemistry: Rat | ||
PMID | Dilution | Publication |
38177103 | 1:100 | Bapat, O., et al. 2024. VAP spatially stabilizes dendritic mitochondria to locally support synaptic plasticity. Nature Communications, 205. |
Immunohistochemistry: Rat | ||
PMID | Dilution | Publication |
33449375 | 1:250 | Deardoff, A.S., et al. 2021. Location, location, location: the organization and roles of potassium channels in mammalian motoneurons. Journal of Physiology, 1391-1420. |