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Immunoblot against membranes from wild- type (N4+/+) or NKCX4 knockout (N4-/-) mouse brains probed with N414/25 TC supe. Data courtesy of Sunita Sharma and Jonathan Lytton (University of Calgary).Adult rat hippocampus immunohistochemistry.
Serial dilutions of BSA-conjugated modified and unmodified peptides dotted onto membrane and probed with N161/20 TC supe (left) and a mouse loading control (right).
Immunoblot on crude membrane fractions from whole mouse (MBM) and rat (RBM) brain and from human hippocampus [HBM(H)], cerebral cortex [HBM(Cx)] and cerebellum [HBM(Cb)] probed with N449/73 (left) and N52A/42 (right) TC supe.Adult rat brain membrane immunoblot.
Adult rat hippocampus immunohistochemistry.Neuronal immunoblot: lysates from neocortical neurons cultured under amino acid starvation and probed with rabbit polyclonal anti-SNAT1 (left) and N104/32 (right). Data courtesy of Jeffery Erickson (LSUHSC)..
Adult rat brain membrane (RBM) immunoblot.Surface expression of SNAT1 and SNAT2 in HCC1806 cells starved of amino acids over 24 h. Image from publication CC-BY-4.0. PMID:36210829

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