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Array tomography immunofluorescence of a single 70 nm section (left) and volume reconstruction of 8 serial sections (right) of LRWhite-embedded adult mouse cerebellum with L118/66 (VGAT, green), rabbit mAb GAD2 (Cell Signaling #5843, magenta) and DAPI (blue).  Purkinje cells (PC) have a low level of VGAT immunoreactivity, and numerous inhibitory synaptic varicosities in molecular (M) and granule (G) layers are colabeled with VGAT and GAD2. Images courtesy of Kristina Micheva (Stanford)..Adult rat brain hippocampus immunohistochemistry.
Adult rat brain striatum and globus pallidus immunohistochemistry.Adult rat hippocampus immunohistochemistry.
Transfected cell immunoblot: extracts of COS cells transiently transfected with untagged VGlut1, VGlut2, VGlut3 or Kv2.1 plasmid and probed with N29/29 (left) or N52A/42 (right) TC supe.Immunoblot versus crude brain membranes from adult mouse (MBM), rat (RBM) and human hippocampus [HBM(H)], cerebral cortex [HBM(Cx)] and cerebellum [HBM(Cb)] probed with N29/29 (left) and N52A/42 (right) TC supe.
NeuroMab Anti-VGlut2 Antibody
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Adult rat brain immunohistochemistry.Adult rat brain membrane immunoblot.
Adult rat brain immunohistochemistry.Adult rat brain membrane immunoblot.

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