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Western blot image of human autophosphorylated TRPM7 C-terminal kinase domain (lanes 1-6). The blot was treated with lambda phosphatase to dephosphorylate TRPM7 phosphosites (lanes 2, 4, & 6). The blot was probed with rabbit polyclonals anti-TRPM7 (Ser-1513) phospho-specific (lanes 1 & 2), anti-TRPM7 (a.a.1484-1497) TP5691 antibody (lanes 3 & 4), or anti-TRPM7 (Ser-1493) phospho-specific (lanes 5 & 6).
Immunohistochemistry of Formalin-Fixed Paraffin-Embedded (FFPE) prostate cancer cells showing specific staining of TRPM8. Western Blot of transfected COS cells showing specific immunolabeling of TRPM8.
Tissue and transfected cell immunoblot: extracts of rat brain membranes (RBM) and of COS cells transiently transfected with HA-tagged mouse and human TRPML3 (mTRPML3 and hTRPML3) or untagged Kv2.1 plasmid and probed with N268/19 (left) and K89/34 (right) TC supe.
Immunofluorescence staining of adult dorsal root ganglion sections from TrpV1 WT and -/- mice with N221/17 TC supe (red), Kv1.2 rabbit polyclonal (green) and Hoechst nuclear stain (blue). Images courtesy of Matthew Rasband (Baylor College of Medicine).Adult rat spinal cord dorsal horn immunohistochemistry.

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