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Figure 1. Jurkat cells were treated with a negative control (left) or staurosporine (middle), then stained using Intracellular GSH Assay (cat. 9137). Cells were read on a flow cytometer using an FL1 99% (2 log) attenuation filter. The median fluorescence intensity of negative control cells was 425,971 in FL1-A (left: Negative), and 289,169 in the induced cells (middle: Positive), a decrease of >30%. Overlay: green: Negative, red: Positive. Data courtesy of Ms. T. Hanson, ICT, 216:52, 051612.

ImmunoChemistry Technologies Intracellular GSH Assay Kit

$246

Figure 1. Jurkat cells were stained with the Intracellular Total ROS Green dye (cat. 9144), then treated with a negative control (left) or 100 µM tert-Butyl hydroperoxide (ROS-inducing agent) (middle). The median fluorescence intensity (MFI) of stained negative control cells was 11,875 in FL1-A (Negative) and 419,067 for ROS-induced cells (Positive). The data are also shown in overlay (black: Negative, red: Positive). Data courtesy of Dr. Kristi Strandberg, ICT, 224:76.

ImmunoChemistry Technologies Intracellular Total ROS Activity Assay

$352

Figure 1. Jurkat cells were treated with DMSO (negative control) or 1 µM staurosporine for 2 hours to induce apoptosis. Cells were then stained with JC-1 dye. Normal healthy cells (two cells, upper and lower left) concentrate JC-1 in mitochondria and fluoresce bright orange. Apoptotic cells (three cells, right), with mitochondria of various stages of permeability, exhibit a reduced orange fluorescence and increased green fluorescence, as JC-1 disperses through the cells. (Dr. Brian Lee, ICT).

Figure 2. Jurkat and HL-60 cells were exposed to DMSO (negative control) (dark orange) or 50 μM CCCP depolarizing agent (light orange) and then incubated with JC-1 for 20 minutes at 37°C, and washed. Aliquots were analyzed in triplicate in a fluorescence plate reader set at 488 nm excitation and 590 nm emission filter settings. Healthy cells exhibited a high level of orange fluorescence; the CCCP-depolarized cells had reduced orange fluorescence as the dye dispersed. (Ms. Tracy Hanson, ICT)

ImmunoChemistry Technologies JC-1 Fluorescent Mitochondrial Membrane Depolarization Assay

From $232.80

ImmunoChemistry Technologies Lysozyme Biotin, Conjugated

$246

Figure 1. Cresyl Violet Perchlorate Excitation and Emission Spectra in Ethanol

Figure 2. MCF-7 cells were exposed to 0.15 µM camptothecin (cat. 6210) for 24 hours at 37°C, then stained with MR-(DEVD)2 for 30 minutes at 37°C, washed, and then stained with 1 µg/mL Hoechst stain. Apoptotic cells with orange-red lysosomal bodies and less intense blue nuclei are intermixed with non-apoptotic cells bearing bright blue nuclei and absent or reduced orange-red lysosomal staining. Photo provided by Dr. Zbigniew Darzynkiewicz at Brander Cancer Research Center Institute, New York, NY.

ImmunoChemistry Technologies Magic Red® Fluorescent Caspase-3/7 Assay Kit

From $205.70

Figure 2. MCF-7 cells were stained with acridine orange for 30 minutes, then washed twice in PBS (cells not stained with Magic Red®) and then analyzed by fluorescence microscopy at 400X using either 492 nm excitation with a 540-550 nm emission filter (A, lysosomes appear yellowish green), or 540 nm excitation with a long pass >640 nm barrier filter (B, lysosomes appear red). Data from the laboratory of Dr. Z. Darzynkiewicz (Brander Cancer Research Center Institute, New York City, NY).

ImmunoChemistry Technologies Magic Red® Fluorescent Cathepsin B Assay Kit

From $212.20

Figure 2. Intracellular cathepsin activity was detected in HL60 cells using Magic Red®-(LR)2 cathepsin K fluorogenic substrate. Intracellular localization of the hydrolyzed fluorescent Magic Red® product was detected using a Nikon Eclipse E800 photomicroscope equipped with a 510–560 nm excitation filter and a 570–620 nm emission/barrier filter at 500X (A). Photo at right (B) shows the corresponding DIC image of the cells. Data courtesy of Dr. Brian Lee, ICT, 061202.

ImmunoChemistry Technologies Magic Red® Fluorescent Cathepsin K Assay Kit

From $212.20

ImmunoChemistry Technologies Magic Red® Fluorescent Cathepsin L Assay Kit

From $212.20

ImmunoChemistry Technologies Methyl Green

From $60

ImmunoChemistry Technologies Methylene Blue Counter Stain

From $60

Figure 1. Jurkat cells were stimulated with Staurosporine for 3 hours (B) or DMSO (A). Mito Flow reagent was added according to the protocol, incubated for 30 minutes and analyzed by flow cytometry: Ex:488nm Em: FL2 channel.

ImmunoChemistry Technologies Mito Flow - Mitochondrial membrane potential detection kit

From $288

Antibodies Incorporated Mitotic Spindle Positive Control

$67

ImmunoChemistry Technologies Monster Block ELISA Blocking Buffer

From $54

ImmunoChemistry Technologies Mouse Serum

$70

Figure 1. Assay principle: a non‐fluorescent detection reagent is oxidized in the presence of hydrogen peroxide and MPO to produce its fluorescent analog.

Figure 2. A MPO standard curve was prepared and run, as described in the protocol, in the absence (A) or presence (B) of 20 mM catalase inhibitor. MPO standard and reaction mix were added to a 96-well black plate and incubated at room temperature in the dark for 30 minutes. The wells were read using Ex: 530nm and Em: 590nm. There is an approximately 50% reduction in signal in the presence of 20mM catalase inhibitor (note the different scales on the x-axis).

ImmunoChemistry Technologies Myeloperoxidase Detection Kit

$522

ImmunoChemistry Technologies Native Human Cathepsin D

$356.40

Figure 1. Brain abscesses were induced in mice following inoculation of live S. aureus. Mice received i.v. injections of DyLight® 690-VAD-FMK tracer or DyLight® 690 Dye Control (cat. 9113) 17 hours post-infection; brain tissue was imaged 1 hour later ex vivo using an IVIS® Spectrum™ (Caliper Life Sciences). Strong caspase activity was seen in brain abscesses using DyLight® 690-VAD-FMK tracer (right); minimal signal was detected in the DyLight® 690 Free Dye Control (left).

ImmunoChemistry Technologies Near Infrared DyLight® 690 Free Dye Control Assay

$312

Figure 1. Balb/c mice were either inoculated with HSV-1 virus, or given a sham treatment. Seven days later, the mice were injected IV with either DyLight® 747-VAD-FMK (kit 9114), NIR-DyLight® 747 Free Dye (kit 9115), or no reagent. Strong caspase activity was seen in the brain of the HSV-1 infected / NIR-DyLight® 747 Tracer-treated mouse. The liver is the route of clearance for DyLight® Tracers. All mice show fluorescence signal in the tail where reagent is likely to pool after IV injection.

ImmunoChemistry Technologies Near Infrared DyLight® 747 Free Dye Control Assay

$312

Experimental design of Aim2WT or Aim2−/− bone marrow (BM) transplantation to Ldlr−/−:Mx1cre:c-mybfl/fl mice receiving i.v. DNA (top) and flow cytometry histograms (bottom) of caspase-1 activity(Assay kit, Cat no. 9122) in bone marrow recipients 24 h after i.v. DNA. Image from publication, CC-BY-4.0, PMID: 39112714.

ImmunoChemistry Technologies Near-Infrared DyLight® 690 Tracer in vivo Active Caspase (VAD) Assay

$452

Figure 1. Apoptotic tissues in murine tumor models were imaged in vivo with 747-VAD-FMK (cat. 9114). Negative control (placebo) mice (left) and experimentally treated mice (right) were injected intravenously with DyLight® 747-VAD-FMK tracer and imaged non-invasively at various time points with the CRi Maestro™ imaging system. Apoptotic tumor tissues fluoresce bright blue in the experimental group subjects, indicating that the experimental treatment had an apoptotic effect.

ImmunoChemistry Technologies Near-Infrared DyLight® 747 Tracer in vivo Active Caspase (VAD) Assay

$452

Figure 1. Using FAM-FLICA®, non-apoptotic cells (unstained, left of histogram) can easily be distinguished from apoptotic cells exhibiting green fluorescence (right of histogram). Jurkat cells were either treated with DMSO as control (A), or 1 µM staurosporine (B) to induce apoptosis. Cells were stained with FAM-FLICA® and analyzed using a flow cytometer in FL-1. Only 7% of non-induced cells (A, right) are apoptotic compared with 96.5% of the induced cells (B, right). (ICT 226:17-19.)

Figure 2. Using 7-AAD, live cells (unstained, left side of each histogram) can easily be distinguished from dead/membrane compromised cells exhibiting red fluorescence (right side of each histogram). Jurkat cells were either untreated (A), or treated with 90% ethanol for 60 seconds, then stained with 7-AAD, and analyzed using a flow cytometer in FL-3. Only 4% of untreated cells (A) are dead compared with 97.6% of the treated cells (B). Data courtesy of Dr. Kristi Strandberg, ICT 226:30-31.

ImmunoChemistry Technologies Necrosis vs Apoptosis Assay Kit

From $351

ImmunoChemistry Technologies Neptune Assay Diluent

From $59

Figure 1. A pool of Jurkat cells was spiked with FAM-YVAD-FMK (cat. 9146) and divided into separate treatment groups. Starting with 24 hour samples and working backwards, 10 µM Nigericin was added to cells and the samples were incubated at 37°C throughout the induction process. The cells were washed and analyzed on a flow cytometer. The level of caspase-1 activity directly correlated to the period of exposure to Nigericin Data. courtesy of Mrs. Tracy Murphy, ICT (220:78).

ImmunoChemistry Technologies Nigericin

$46.60

Figure 1. Typical standard curves. A new curve must be generated each time the assay is run.

Figure 2. Typical nitrite linearity curve

ImmunoChemistry Technologies Nitric Oxide Colorimetric Detection Kit

$350

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