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Figure 1. Jurkat cells were stained with DAF-2DA dye (Kit 9155), washed, and then treated with DMSO control (left histogram) or 1 mM DEA NONOate, a nitric oxide donor (middle histogram). Cells were read on the FL1 channel of a flow cytometer. The median fluorescence intensity (MFI) of DEA NONOate treated cells was 3.5-fold higher than for the control cells. Data also overlaid in a single plot (right, black: Negative; right, red: Positive). Data courtesy of Dr. Kristi Strandberg, ICT, 227:76.

Figure 2. Jurkat suspension cells were stained with DAF-2DA dye, washed, and then treated with DMSO control (Panels A-C) or DEA NONOate (Panels D-F), a nitric oxide donor. Cells treated with DEA NONOate showed an increased level of green fluorescence relative to the untreated cells (Panels A v and D). Microscope images were obtained using a Nikon Eclipse 90i microscope with a Hamamatsu Flash 4.0 camera. Data courtesy of Dr. Kristi Strandberg (ICT 228:57-58).

ImmunoChemistry Technologies Nitric Oxide Synthase Assay

$223

Aves Labs Non-Immune Chicken IgY Secondary Antibody

$102

ImmunoChemistry Technologies NP-40

From $90

ImmunoChemistry Technologies Nuclear Fast Red

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Antibodies Incorporated Nucleolar Positive Control

$67

ImmunoChemistry Technologies One step Polymer HRP Goat IgG (H+L)

From $293

ImmunoChemistry Technologies One step Polymer HRP Goat with DAB

From $289

ImmunoChemistry Technologies One Step Polymer HRP Mouse and Rat IgG (H+L)

From $293

ImmunoChemistry Technologies One step Polymer HRP Mouse and Rat IgG with DAB

From $289

ImmunoChemistry Technologies One step Polymer HRP Mouse, Rabbit & Rat (H+L) Detection Kit

From $340

ImmunoChemistry Technologies One step Polymer HRP Mouse, Rat and Rabbit with DAB

From $335

ImmunoChemistry Technologies One step Polymer HRP Rabbit IgG (H+L) IHC Detection Kit

From $293

ImmunoChemistry Technologies One step Polymer HRP Rabbit IgG with DAB

From $289

ImmunoChemistry Technologies Organo / Limonene Mount™

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ImmunoChemistry Technologies Pack of Desiccant Packets

$8

ImmunoChemistry Technologies Pepsin Reagent (Ready to Use)

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ImmunoChemistry Technologies Peroxidase Signal Enhancer Kit

$117

ImmunoChemistry Technologies Peroxidase Stabilizing Buffer

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Figure 1. (a) Fluorescent responses of NP3 (5 μM) toward various analytes (10 μM). Data shown represent fluorescent intensity at 470nm, 30 min after addition of the analytes. (b) ONOO− (final 10 μM) was quickly injected into a solution of NP3 (final 5μM), and the fluorescent intensity at 470 nm was plotted against time. (c) Fluorescence enhancement of NP3 (5μM) at 470 nm as a function of ONOO− (0−10 μM) after 15 min of reaction. All data acquired in PBS (10 mM, pH 7.4) with excitation at 375 nm.

Figure 2. (a) Time-lapse images taken from living EA.hy926 endothelial cells. Cells were preincubated with NP3 (5.0 μM), followed by stimulation with or without SIN-1 (0.5 mM). (b) Dynamic changes of NP3 fluorescence after SIN-1 (0.5 mM) treatment in panel a. (c) Effects of ONOO− scavengers uric acid (100 μM) and FeTTPS (1 μM) on changes in NP3 fluorescence in endothelial cells in the presence of ONOO− (60 μM). PI (red) stains nuclei. NP3 fluorescence was collected at 420−480 nm with λex 405 nm.

ImmunoChemistry Technologies Peroxynitrite Detection Kit

$298

ImmunoChemistry Technologies Phosph-Free Block ELISA Blocking Buffer

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ImmunoChemistry Technologies Phosphate Buffer, 10X

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ImmunoChemistry Technologies Phosphate Buffered Saline (PBS), 10X

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Antibodies Incorporated Phosphate Buffered Saline Solution (PBS), Dry

$31

ImmunoChemistry Technologies Plasma Sample Diluent

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