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Immunoblot against isolated cilia and extracts of COS cells transiently transfected with GFP-tagged DNAIC1 or untagged Kv2.1 plasmid probed with UNC 65.56.18.11 (left) or N52A/42 (right) TC supe.
Adult rat brain membrane immunoblot.
Adult rat brain immunohistochemistry.Transfected cell immunoblot: extracts of CV-1 fibroblast cells transfected with VGlut3 (+) or left untransfected (-) and probed with N34/34 TC supe. Image courtesy of Jeff Erickson, Louisiana State University Health Sciences Center.
Immunoblot against crude brain membrane preparations from adult rat (RBM) or wild-type (WT) or KA2 knockout (KO) mice probed with N279B/27 (left) or N52A/42 (right) TC supe. Mouse brains courtesy of Anis Contractor (Northwestern University.
Immunoblot versus crude membranes from adult rat brain (RBM) and WT and TRIP8b KO mouse brains probed with N212/17 (left) and N52A/42 (right) TC supe. Mouse samples courtesy of Dane Chetkovich (Northwestern University).Adult rat hippocampus immunohistochemistry.
Adult rat brain membrane immunoblotRepresentative images of EDL muscles of 3 M, 12 M, and 24 M-old mice, whole mount stained with 555-anti-LRP4 (Cat no 75-221, 1:200, red) and NF/Syn (blue). Arrowheads, non-annulospiral axons had no LRP4 expression. Arrows, LRP4 negative annulospiral endings in aged mice. Scale bar, 10 µm. Image from publication CC-BY-4.0. PMID:36765071
Adult rat hippocampus immunohistochemistry.Adult rat brain membrane immunoblot.
Immunoblot against membranes from livers of wild-type (WT) and VDAC1 knockout (KO) mice and brains of WT mice (MBM) and rat (RBM). Liver samples courtesy of Tatiana Sheiko and Bill Craigen (Baylor College of Medicine).Array tomography immunofluorescence of 70 nm sections of LRWhite-embedded adult mouse hippocampus. Two consecutive sections labeled with N152B/23 (bottom) and DAPI (top), where low level autofluorescence reveals mitochondria appearing as brighter elongated structures within darker apical dendrites (arrows). Images courtesy of Kristina Micheva (Stanford).
Adult rat cerebellar immunohistochemistry.Representative confocal images of control and α-Syn fibril-treated mouse neurons stained with IP3R1 (cat. 75-035; magenta) and MAP2 (cyan) with or without treatment of 100 nM UNC-3230. Zoomed-in images show only IP3R1. Image from publication CC-BY-4.0. PMID:37838947
Adult rat hippocampus immunohistochemistry.Immunoblot on adult rat brain membrane (RBM) and lysates from Kvβ1.1 -transfected (COS+) and untransfected (COS-) COS cells.
Adult rat basal forebrain immunofluorescence with antigen retrieval via sodium citrate pretreatment with K37/89 (red) and Kv2.1 rabbit (green). Image courtesy of Kaori Misonou and Hiroaki Misonou (University of Maryland, now at Doshisha University, Japan).Immunoblot against adult rat brain membranes (RBM) and extracts of COS cells transiently transfected with Myc-tagged Kv2.2short, GFP-tagged Kv2.2long or untagged Kv2.1 plasmid probed with K37/89 (left), K89/34 (middle) or N52A/42 (right) TC supe.
Centromere Positive Control
ANA Kit (20 x 12-well slides)
Immunoblot versus crude membranes from adult rat brain (RBM) and lysates of WT and genetically engineered Vapa-/- 3A11 RAW 264.7 cells probed with N479/12 (left) or N52A/42 (right) TC supe.  Cell lines courtesy of Tim Schaiff, Sanghyun Lee and Skip Virgin (Washington University, 2017 McCune et al mBio, PMID 28698274).
Immunofluorescence labeling of adult rat hippocampus with N450/53 TC supe (red) and Hoechst nuclear stain (blue)..Anti-BDNF Antibody
Adult rat pontine nucleus immunohistochemistry.Adult rat hippocampus immunohistochemistry.
Adult rat hippocampus immunohistochemistry.Transfected cell immunoblot: extracts of COS-1 cells transiently transfected with Shank1, Shank2, Shank3 or Kv2.1 plasmids and probed with N69/46 TC supe (left panel) or a monoclonal lane loading control (right panel).
NeuroMab Anti-Shank3 Antibody
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Adult rat hippocampal immunohistochemistry.Adult rat cerebellar immunohistochemistry.
Adult mouse brain immunoblot: extracts of brains from wild-type (1), Netrin-G2 knockout (2) and NGL-2 knockout (3) mice were probed with Netrin-G2 antibody (left) or N50/36 (right). Reproduced with permission from 2007 Zhang et al Genes Brain Behav.Adult rat brain membrane (RBM) and transfected cell immunoblot: extracts of RBM and COS cells transiently transfected with Myc-tagged NGL-1, NGL-2, NGL-3 or untagged Kv2.1 plasmid and probed with N50/36 (left) or K89/41 (right) TC supe.

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