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Immunoblot versus crude membranes from adult rat brain (RBM) and WT and TRIP8b KO mouse brains probed with N212/17 (left) and N52A/42 (right) TC supe. Mouse samples courtesy of Dane Chetkovich (Northwestern University).Adult rat hippocampus immunohistochemistry.
Adult rat brain membrane immunoblotRepresentative images of EDL muscles of 3 M, 12 M, and 24 M-old mice, whole mount stained with 555-anti-LRP4 (Cat no 75-221, 1:200, red) and NF/Syn (blue). Arrowheads, non-annulospiral axons had no LRP4 expression. Arrows, LRP4 negative annulospiral endings in aged mice. Scale bar, 10 µm. Image from publication CC-BY-4.0. PMID:36765071
Adult rat hippocampus immunohistochemistry.Adult rat brain membrane immunoblot.
Adult rat brain membrane immunoblot.Lysates of rat cortical neurons treated with the indicated compounds were  subjected to ABE to purify palmitoylated proteins. Levels of palmitoyl-AnkG-190 (right blot) and total AnkG (cat. 75-187) expression in parent lysates (left blot bottom blot) were detected with specific antibodies. Exclusion of NH2OH was used as a control for assay specificity. Image from publication CC-BY-4.0. PMID:36969554
Adult rat hippocampus immunohistochemistry.Immunoblot on adult rat brain membrane (RBM) and lysates from Kvβ1.1 -transfected (COS+) and untransfected (COS-) COS cells.
Adult rat brain immunohistochemistry.Adult rat brain membrane immunoblot.
Adult rat basal forebrain immunofluorescence with antigen retrieval via sodium citrate pretreatment with K37/89 (red) and Kv2.1 rabbit (green). Image courtesy of Kaori Misonou and Hiroaki Misonou (University of Maryland, now at Doshisha University, Japan).Immunoblot against adult rat brain membranes (RBM) and extracts of COS cells transiently transfected with Myc-tagged Kv2.2short, GFP-tagged Kv2.2long or untagged Kv2.1 plasmid probed with K37/89 (left), K89/34 (middle) or N52A/42 (right) TC supe.
Immunolabeling of spreaded WT mouse pachytene spermatocytes positively labeling CREST/Centromere Protein (red, Cat 15-235, 1:20) and SYCP3 (green). Image kindly provided by Fernando Sánchez Sáez and Alberto M. Penás from Universidad de Salamanca, Spain.Immunostaining of Vipera berus (Reptile, snake) synaptonemal complexes identifying the lateral elements of meiotic bivalents (Anti-SYCP3, green), the centromere proteins (Anti-ACA, Cat 15-235, 1:250, red), and nuclei staining with DAPI (blue). Image kindly provided by Victor Spangenberg, Vavilov Institute of General Genetics, Moscow, Russia.
Centromere Positive Control
ANA Kit (20 x 12-well slides)
Immunoblot versus crude membranes from adult rat brain (RBM) and lysates of WT and genetically engineered Vapa-/- 3A11 RAW 264.7 cells probed with N479/12 (left) or N52A/42 (right) TC supe.  Cell lines courtesy of Tim Schaiff, Sanghyun Lee and Skip Virgin (Washington University, 2017 McCune et al mBio, PMID 28698274).
Immunofluorescence labeling of adult rat hippocampus with N450/53 TC supe (red) and Hoechst nuclear stain (blue)..Anti-BDNF Antibody
Adult rat pontine nucleus immunohistochemistry.Adult rat hippocampus immunohistochemistry.
Adult rat hippocampus immunohistochemistry.Transfected cell immunoblot: COS cells transiently transfected with Kv2.1 and Ataxin-1 plasmids and probed with N76/3 (left panel) and K89/34 (right panel) TC supe.
Adult rat hippocampus immunohistochemistry.Transfected cell immunoblot: extracts of COS-1 cells transiently transfected with Shank1, Shank2, Shank3 or Kv2.1 plasmids and probed with N69/46 TC supe (left panel) or a monoclonal lane loading control (right panel).
NeuroMab Anti-Shank3 Antibody
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Adult rat hippocampal immunohistochemistry.Adult rat cerebellar immunohistochemistry.
Adult mouse brain immunoblot: extracts of brains from wild-type (1), Netrin-G2 knockout (2) and NGL-2 knockout (3) mice were probed with Netrin-G2 antibody (left) or N50/36 (right). Reproduced with permission from 2007 Zhang et al Genes Brain Behav.Adult rat brain membrane (RBM) and transfected cell immunoblot: extracts of RBM and COS cells transiently transfected with Myc-tagged NGL-1, NGL-2, NGL-3 or untagged Kv2.1 plasmid and probed with N50/36 (left) or K89/41 (right) TC supe.
Immunoblot against extracts of COS cells transiently transfected with GFP-tagged zebrafish VSP (DrVSP) or untagged Xenopus (XtVSP, XlVSP and XlVSP2), sea squirt (CiVSP), chicken VSP (GgVSP) or Kv2.1 plasmid probed with N432/21 (left) or N52A/42 (right) TC supe..II Confocal images of Dr-Vsp immunostaining in a 7-dpf wild-type zebrafish larva (sagittal section). Dr-Vsp is highly expressed in enterocytes at the posterior part of the mid-intestine, which are defined as lysosomerich enterocytes (LREs). Image from publication, CC-BY-4.0, PMID: 36088390
Adult rat brain immunohistochemistry.Transfected cell immunoblot: extracts of CV-1 fibroblast cells transfected with VGlut3 (+) or left untransfected (-) and probed with N34/34 TC supe. Image courtesy of Jeff Erickson, Louisiana State University Health Sciences Center.
Adult rat hippocampus immunohistochemistry.Immunoblot versus samples from adult rat brain (RBLSP) and WT and BAF53b KO mouse brains probed with N332B/15 (left) and N336B/83 (right) TC supes. Mouse samples courtesy of Ann Kuo and Gerald Crabtree (Stanford).
Adult rat brain immunohistochemistry.Immunoblot against crude membrane fractions from whole mouse (MBM) or rat (RBM) brain and from human hippocampus [HBM(H)], cerebral cortex [HBM(Cx)] or cerebellum [HBM(Cb)] and probed with N25/35 (left) or N52A/42 (right) TC supe.

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