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Whole brain immunohistochemistry.Adult rat hippocampus immunohistochemistry.
Adult rat hippocampus immunohistochemistry.Whole brain immunohistochemistry.
Transfected cell immunoblot: extracts of COS cells transiently transfected with GFP-tagged NR2A, NR2B or untagged Kv2.1 plasmid and probed with N327A/38 (left) and N86/38 (right) TC supe.
Transfected cell immunoblot: extracts of COS cells transiently transfected with GFP- tagged Synaptotagmin or untagged Kv2.1 plasmids and probed with N278/19 (left) and N86/8 (right).Adult rat brain membrane immunoblot.
Immunolabeling of mouse skeletal muscle staining NaV 1.4 (Cat 75-424, 1:1000, red) and acetylcholine receptor (red). The frozen tissue section was fixed with 4% PFA for 10 minutes and mounted before staining.  Image kindly provided by Chengjie Xie and Lauren Fish, Brown University.
Adult rat brain membrane immunoblot.Transfected cell immunofluorescence staining N22/21=green, rabbit anti-Shank1=red, DAPI=blue.
Adult rat brain membrane immunoblot.
Immunoblot against crude membrane fractions from whole mouse (MBM) or rat (RBM) brain and from human hippocampus [HBM(H)], cerebral cortex [HBM(Cx)] or cerebellum [HBM(Cb)] and probed with N202/7 (left) or N52A/42 (right) TC supe.Adult rat brain immunohistochemistry.
Transfected cell immunoblot: extracts of COS cells transiently transfected with Myc-tagged Frataxin and untagged Kv2.1 plasmids and probed with N191/7 (left), 19E10 (middle) and N52A/42 (right) TC supe.
Adult rat striatum immunohistochemistry.
Immunoblot against membranes from livers of wild-type (WT) and VDAC1 knockout (KO) mice and brains of WT mice (MBM) and rat (RBM). Liver samples courtesy of Tatiana Sheiko and Bill Craigen (Baylor College of Medicine).Array tomography immunofluorescence of 70 nm sections of LRWhite-embedded adult mouse hippocampus. Two consecutive sections labeled with N152B/23 (bottom) and DAPI (top), where low level autofluorescence reveals mitochondria appearing as brighter elongated structures within darker apical dendrites (arrows). Images courtesy of Kristina Micheva (Stanford).
Cerebellar lysate immunoblot probed with N151/3 (3 mg/mL).Cultured rat hippocampal neuron surface (S, left) and total (T, right) protein immunofluorescence staining (green) with N151/3 (2 mg/mL).
Transfected cell immunoblot: extracts of COS cells transiently transfected with GFP-tagged Gamma- protocadherin-A3 and -A4 and untagged Kv2.1 plasmids and probed with N144/17 (left) and N159/5 (right) TC supe.
Transfected cell immunoblot: extracts of COS cells transiently transfected with GFP-tagged Gamma-protocadherin-B2 and -B1 and untagged Kv2.1 plasmids and probed with N148/30 (left) and N86/6 (right) TC supe.
Immunolabeling of mouse hippocampus identifying GKAP (Anti-SAPAP, Cat no 75-156, green). Nuclear staining done with DAPI.  Image kindly provided by Huaye Zhang, Department of Neuroscience and Cell Biology, Rutgers Robert Wood Johnson Medical School. Adult RBM and transfected cell immunoblot: extracts of RBM and COS cells transiently transfected with Flag-tagged SAPAP1, SAPAP4 or untagged Kv2.1 plasmids and probed with N127/31 TC supe.
Adult rat hippocampus immunohistochemistry.Immunoblots versus crude adult rat brain membranes (RBM) and brain extracts from Thorase WT and KO mice and probed with N125/10 (left) and N52A/42 (right) TC supe. Samples courtesy of Dr. Jianmin Zhang, Dr. Ted Dawson and Dr. Valina Dawson, Johns Hopkins School of Medicine.
Immunoblot against crude membranes from whole mouse (MBM) or rat (RBM) brain and from human hippocampus [HBM(H)], cerebral cortex [HBM(Cx)] or cerebellum [HBM(Cb)] probed with N105/17 (left) or N52A/42 (right) TC supe.Adult rat hippocampus immunohistochemistry.
Adult rat hippocampus immunohistochemistry.Adult rat brain membrane immunoblot.
Immunoblot on adult rat brain membrane (RBM), and adult mouse brain membranes (MBM) from wild-type (WT) and lethargic (lh/lh) mice (left) Lethargic mice courtesy of Dr. Jeff Noebels, Baylor College of Medicine.Adult rat brain membrane immunoblot.
Immunoblot against crude membranes from adult rat brain (RBM), wild-type (MBM-WT) and BKAlpha knockout (MBM- BKaKO) mouse brains probed with L6/23 (left), L6/60 (middle) or N52A/42 (right) TC supe. Reproduced from PLoS One (2010 Wersinger et al, PMID 21079807).Maximum intensity projections of confocal z stacks taken from the apical cochlear region of Eps8−/− (top) and Eps8−/− rescued (bottom) mice using antibodies against BK channels carrying IK,f (red) and the hair-cell marker Myo7a (white). Image from publication CC-BY-4.0. PMID: 36034774
Adult rat brain membrane immunoblot.
Adult rat optic nerve immunofluorescence staining L11A/41 (5 μg/ml)=red Rabbit anti-Caspr=green Image courtesy of Dr. Matthew Rasband.Adult rat brain membrane immunoblot.
Adult rat brain striatum and globus pallidus immunohistochemistry.Adult rat hippocampus immunohistochemistry.

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