Pronase Reagent (Ready to Use)
Pronase is an antigen retriever solution used for proteolytic digestion of formalin-fixed paraffin-embedded (FFPE) tissue sections prior to application of antibodies.
Pronase is an antigen retriever solution used for proteolytic digestion of formalin-fixed paraffin-embedded (FFPE) tissue sections prior to application of antibodies. In IHC most commonly used fixative like formalin mask tissue antigens (cellular, membrane and nuclear) by cross-linking process, this results in poor or no staining in IHC. Pronase digestion of FFPE tissue section improves accessibility of antibodies to tissue antigens.
Ready-to use Pronase reagent
2-8 °C; DO NOT FREEZE
Ships overnight (domestic), International Priority Shipping
For research use only; not for use in diagnostic procedures. FOR IN VITRO LABORATORY USE ONLY
United States
- Deparafinize FFPE tissue section is carried out as outlined in your protocol. Hydrate tissue with PBS or ImmunoBioScience Universal Immuno buffer.
- Remove buffer, add 2-4 drops of Pronase reagent to cover tissue section.
- Incubate at room temp. for 5 minutes (the time required for optimum digestion of FFPE tissue section will vary with the extent of fixation, generally approx. 5 minutes are sufficient at room temp. In some cases it may require 15 minutes at room temp).
- Discard Pronase reagent, wash 3-5 times with buffer.
- The tissue sections are ready for further IHC protocol.
Product Specific References
| PMID | Publication |
| 22217002 | Kameyama, K., et al. 2012. Immunological characterization of Neospora caninum cyclophilin. Parasitology, 294-301. |
| 21699673 | Awadin, W, et al. 2011. Detection of lumpy skin disease virus antigen and genomic DNA in formalin-fixed paraffin-embedded tissues from an Egyptian outbreak in 2006. Transboundary and Emerging Diseases, 451-457. |