Trypsin Reagent
Trypsin is an antigen retriever solution used for proteolytic digestion of formalin-fixed paraffin-embedded (FFPE) tissue sections prior to application of antibodies.
Trypsin is an antigen retriever solution used for proteolytic digestion of formalin-fixed paraffin-embedded (FFPE) tissue sections prior to application of antibodies. In IHC most commonly used fixative like formalin; mask tissue antigens (cellular, membrane and nuclear) by cross-linking process, this results in poor or no staining in IHC. Trypsin digestion improves immunoreactivity of some antigens in FFPE tissue sections. For cytokeratin clone AE3 and AE1/AE3, this enzyme works much better than boiling the tissue with citrate buffer.
This enzyme is supplied as concentrated form along with buffer
2-8 °C; DO NOT FREEZE
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For research use only; not for use in diagnostic procedures. FOR IN VITRO LABORATORY USE ONLY
United States
- Deparafinize FFPE tissue section is carried out as outlined in your protocol. Hydrate tissue with PBS or ImmunoBioScience Universal Immuno buffer. Add endogenous peroxidase block as usual. Wash tissue section 3-5 times with buffer.
- Remove buffer, add 2-4 drops of ready-to use enzyme solution to cover the tissue section.
- Incubate at 37oC for 5-10 minutes or 10-15 minutes at room temperature, (the time required for optimum digestion of FFPE tissue section will vary with the extent of fixation, generally approx. 5-15 minutes are sufficient at the above temperatures. In some cases it may require more time).
- Discard trypsin reagent, wash 3-5 times with buffer.
- The tissue sections are ready for further IHC protocol.
AR-6541-01: 15 mL
Reagent B (Buffer) 15 ml
Reagent E (Enzyme concentrated), 2ml
AR-6541-02: 100 mL
Reagent B (Buffer) 100 ml
Reagent E (Enzyme concentrated), 10ml