ImmunoChemistry Technologies

303 products

ImmunoChemistry Technologies is your partner in cell viability assays, ELISA reagents, IHC reagents, and immunoassay services. With decades of protein chemistry expertise, ICT offers a wide range of products for immunoassay, cellular, and molecular diagnostics development. Our kits include fluorescent whole cell assays for intracellular apoptosis detection, autophagy, oxidative stress, caspase activity, and more. Optimize your technique with our coating and wash buffers, blockers, sample and assay diluents, stabilizers, and stop solutions. From ELISA development and plate coating, to IHC counterstains and mounting media, to antibody conjugation and purification, the team at our ISO-13485-certified facility in Davis, California is dedicated to helping you build a better assay.

Showing 25 - 48 of 303 products

ImmunoChemistry Technologies Immuno Fluoro Mount™ with DABCO

From $80.75

The cell morphology of MDA-MB-231 breast cancer cells after they were treated with fludioxonil(Flu) for 72 h and were stained with DAPI and actin. The samples were sealed with mounting medium (Cat. no. AR-6515). Image from publication, CC-BY-4.0, PMID: 39201710.

ImmunoChemistry Technologies Fluoroshield™ with DABCO

From $85.75

Figure 1. Assay principle. The kit uses a non-fluorescent detection reagent, which is reduced by NADH to produce its fluorescent analog and NAD. NAD is further converted to NADH via an enzyme coupled reaction (which does not react with NADP/NADPH).

Figure 2. NADH standard curve. Titrated in NADH standard diluent. Incubation time was 1 hour.

ImmunoChemistry Technologies Fluoro NAD/NADH Detection Kit by Cell Technology

From $591

Figure 1. (a) Fluorescent responses of NP3 (5 μM) toward various analytes (10 μM). Data shown represent fluorescent intensity at 470nm, 30 min after addition of the analytes. (b) ONOO− (final 10 μM) was quickly injected into a solution of NP3 (final 5μM), and the fluorescent intensity at 470 nm was plotted against time. (c) Fluorescence enhancement of NP3 (5μM) at 470 nm as a function of ONOO− (0−10 μM) after 15 min of reaction. All data acquired in PBS (10 mM, pH 7.4) with excitation at 375 nm.

Figure 2. (a) Time-lapse images taken from living EA.hy926 endothelial cells. Cells were preincubated with NP3 (5.0 μM), followed by stimulation with or without SIN-1 (0.5 mM). (b) Dynamic changes of NP3 fluorescence after SIN-1 (0.5 mM) treatment in panel a. (c) Effects of ONOO− scavengers uric acid (100 μM) and FeTTPS (1 μM) on changes in NP3 fluorescence in endothelial cells in the presence of ONOO− (60 μM). PI (red) stains nuclei. NP3 fluorescence was collected at 420−480 nm with λex 405 nm.

ImmunoChemistry Technologies Peroxynitrite Detection Kit

$289

Figure shows the two phosphate moieties of ADP, with no trace of a peak indicating the third phosphate of ATP using P31 NMR.

ImmunoChemistry Technologies Ultra Pure ADP by Cell Technology

From $393

ImmunoChemistry Technologies Sample Diluent Optimization Pack

$187

ImmunoChemistry Technologies Protein-Free Sample Diluent

From $46.75

Figure 1. Stabilization of HRP conjugates

Figure 2. Minimizes background in mono-poly sandwich ELISAs

ImmunoChemistry Technologies HRP Conjugate Stabilizer, Non-Mammalian-based, 1X

From $68.25

ImmunoChemistry Technologies Alternative Block ELISA Blocking

From $52.25

ImmunoChemistry Technologies 96-Well Plate Template Post-It Notes

$7.80

ImmunoChemistry Technologies Stop Solution for TMB Substrates (STOPT)

From $29.90

Figure 1. Dilute samples within the detection limits of the ELISA

ImmunoChemistry Technologies Sample Diluent - Non-Mammalian-based

From $57.50

Figure 1. Use of ELISA Blocking Buffer in Antigen-Down and Sandwich ELISA formats

ImmunoChemistry Technologies ELISA Blocking Buffer

From $52.25

Figure 1. FLICA® 660 was reconstituted in DMSO, diluted in diH2O, and analyzed in a fluorescence plate reader. The excitation spectrum was generated using an emission of 760 nm. The emission spectrum was generated using an excitation of 600 nm.

Figure 2. Jurkat cells were treated with a negative control (left) or staurosporine, an apoptosis-inducing agent (right), for 4 hours, then stained with FLICA® 660-DEVD-FMK (cat. 9125) for 1 hour. Cells were washed and analyzed by flow cytometer. The negative control induced caspase-3/7 activity in 4.4% of cells (left); treatment with staurosporine induced caspase-3/7 activity in 70.9% of cells (right). This is a ratio of 16:1. Data courtesy of Mrs. Tracy Murphy, ICT, 13F38, 022013.

ImmunoChemistry Technologies Far-Red Fluorescent FLICA® 660 Caspase-3/7 (DEVD) Assay Kit

$256

Figure 1. Cresyl Violet Perchlorate Excitation and Emission Spectra in Ethanol

Figure 2. MCF-7 cells were stained with acridine orange for 30 minutes, then washed twice in PBS (cells not stained with Magic Red®) and then analyzed by fluorescence microscopy at 400X using either 492 nm excitation with a 540-550 nm emission filter (A, lysosomes appear yellowish green), or 540 nm excitation with a long pass >640 nm barrier filter (B, lysosomes appear red). Data from the laboratory of Dr. Z. Darzynkiewicz (Brander Cancer Research Center Institute, New York City, NY).

ImmunoChemistry Technologies Magic Red® Fluorescent Cathepsin L Assay Kit

From $206

Figure 2. Intracellular cathepsin activity was detected in HL60 cells using Magic Red®-(LR)2 cathepsin K fluorogenic substrate. Intracellular localization of the hydrolyzed fluorescent Magic Red® product was detected using a Nikon Eclipse E800 photomicroscope equipped with a 510–560 nm excitation filter and a 570–620 nm emission/barrier filter at 500X (A). Photo at right (B) shows the corresponding DIC image of the cells. Data courtesy of Dr. Brian Lee, ICT, 061202.

ImmunoChemistry Technologies Magic Red® Fluorescent Cathepsin K Assay Kit

From $206

Figure 1. Control Alzheimer's Disease (AD) macrophages and curcumin-treated AD macrophages were exposed to FITC-labeled Aß and stained with SR-DEVD-FMK (#931/932). Macrophages that engulf FITC-Aß fluoresce green (right). Caspase-positive cells fluoresce red (left). Essentially all of the untreated AD macrophages engulfed FITC-Aß and became apoptotic. Curcumin-treated AD macrophages engulfed FITC-Aß but did not become apoptotic. Data courtesy of Dr. Milan Fiala, UCLA 072205.

ImmunoChemistry Technologies Red Fluorescent SR-FLICA® Caspase-3/7 (DEVD) Assay Kit

From $216

ImmunoChemistry Technologies UEA-I-FITC, Conjugated

$194.50

ImmunoChemistry Technologies UEA-I-Biotin, Conjugated

$194.50

ImmunoChemistry Technologies PNA-R-PE, Conjugated

$227

ImmunoChemistry Technologies PNA-FITC, Conjugated

$194.50

ImmunoChemistry Technologies PNA-Biotin, Conjugated

$194.50

ImmunoChemistry Technologies ConA-FITC, Conjugated

$194.50

ImmunoChemistry Technologies ConA-Biotin Conjugated

$171.50

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