Anti-Akt (N-terminal region) Antibody (M101)

4 Citations

Our Akt (N-terminal region) primary antibody from PhosphoSolutions is mouse monoclonal. It detects human, mouse, and rat Akt (N-terminal region) and is Protein A purified. It is great for use in WB, ICC.

Western blot analysis of A431 cells, serum starved overnight (lanes 1 & 3) or treated with calyculin A (100 nM) for 30 min. (lanes 2 & 4). The blot was probed with anti-Akt (Thr-34) (lanes 1 & 2) or anti-Akt1 (N-terminal region) (lanes 3 & 4).
Western blot analysis of A431 cells untreated (lanes 1 & 3) or treated with 100 ng/ml EGF for 60 min. (lanes 2 & 4). The blots were probed with monoclonal anti-phospho-Akt (Ser-473) (lanes 1 & 2) and monoclonal anti-Akt1 (N-terminal region) (lanes 3 &4).
Western blot image of HEK293 cells treated with 100 nM insulin for 15, 30, 60, and 120 minutes measuring AKT activation between phosphorylated and total AKT protein levels. (Cat No AM1011, 1:1000). CC-BY-4.0 PMID: 37108445
Western blot analysis of A431 cells, serum starved overnight (lanes 1 & 3) or treated with calyculin A (100 nM) for 30 min. (lanes 2 & 4). The blot was probed with anti-Akt (Thr-34) (lanes 1 & 2) or anti-Akt1 (N-terminal region) (lanes 3 & 4).
Roll over image to zoom in Click on image to zoom
Western blot analysis of A431 cells, serum starved overnight (lanes 1 & 3) or treated with calyculin A (100 nM) for 30 min. (lanes 2 & 4). The blot was probed with anti-Akt (Thr-34) (lanes 1 & 2) or anti-Akt1 (N-terminal region) (lanes 3 & 4).
Western blot analysis of A431 cells untreated (lanes 1 & 3) or treated with 100 ng/ml EGF for 60 min. (lanes 2 & 4). The blots were probed with monoclonal anti-phospho-Akt (Ser-473) (lanes 1 & 2) and monoclonal anti-Akt1 (N-terminal region) (lanes 3 &4).
Western blot image of HEK293 cells treated with 100 nM insulin for 15, 30, 60, and 120 minutes measuring AKT activation between phosphorylated and total AKT protein levels. (Cat No AM1011, 1:1000). CC-BY-4.0 PMID: 37108445

Human, Mouse, Rat
ICC, WB
Mouse

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Data Sheet

SKU: AM1011

Volume: 100 µL
1-2 business days
Price:
$285.00

Product Specific References for Applications and Species

Western Blot: Human
PMID Dilution Publication
371084451:1000Cimini, FA, et al. 2023. Dynamic Changes of BVRA Protein Levels Occur in Response to Insulin: A Pilot Study in Humans. International Journal of Molecular Sciences, 7282.
30941654not listedHu, C, et al. 2019. Resveratrol inhibits the proliferation of estrogen receptor-positive breast cancer cells by suppressing EZH2 through the modulation of ERK1/2 signaling. Cell biology and toxicology, 445-456.
Western Blot: Rat
PMID Dilution Publication
380041051:1000Rodrigues Junior, CF, et al. 2023. Changes in Skeletal Muscle Protein Metabolism Signaling Induced by Glutamine Supplementation and Exercise. Nutrients, 4711.
19880666not listedWei, H, et al. 2010. Ischemic preconditioning and heat shock activate Akt via a focal adhesion kinase-mediated pathway in Langendorff-perfused adult rat hearts. American journal of physiology. Heart and circulatory physiology, H152-157.

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