Our Paxillin (Ser-83) rabbit polyclonal phosphospecific primary antibody from PhosphoSolutions is produced in-house. It detects rat, mouse Paxillin (Ser-83) and is antigen affinity purified. It is great for use in WB, ICC.
Western blot analysis of PC12 cells untreated (lanes 1 & 3) or treated with NGF (200 ng/ml) for 60 min (lanes 2 & 4). The blots were probed with mouse monoclonal anti-Paxillin (PM1071; lane 1 & 2) or rabbit polyclonal anti-Paxillin (Ser-83) phospho-specific antibody (lane 3 & 4).
Paxillin, a focal adhesion protein, is involved in focal adhesion formation during cell adhesion and migration. Paxillin contains LD motifs, LIM domains, and SH3-/SH2-binding domains that participate in a variety of protein-protein interactions with kinases, GTPase-activating proteins, and cytoskeletal proteins. Phosphorylation of paxillin occurs at tyrosine, threonine, and serine sites. Serine and threonine phosphorylation of paxillin occur in response to growth-factor activation, PKC activators, and fibronectins. Phosphorylation of Ser-85, Ser-178, and Thr-538 may be important sites for regulating paxillin activity. Paxillin phosphorylation of Thr-538 occurs in response to TPA-activated PKCs in vitro, and this phosphorylation may contribute to dissolution of the actin cytoskeleton and redistribution of LFA-1 integrins in vivo.
Antigen Affinity Purified
Polyclonal
IgG
ICC, WB
Rabbit
PXN
68
Phospho-Paxillin (Ser-83) synthetic peptide (coupled to KLH) corresponding to amino acid residues around serine 83 of mouse paxillin. This sequence is highly conserved in rat and human paxillin and is also found in all isoforms (a, b, g) of paxillin.
Mouse, Rat
Storage at -20°C is recommended, as aliquots may be taken without freeze/thawing due to presence of 50% glycerol. Stable for at least 1 year at -20°C.
Liquid
PBS + 1 mg/ml BSA, 0.05% NaN3 and 50% glycerol
WB: 1:1000
ICC: 1:50
Unconjugated
The antibody detects a 68kDa* protein corresponding to the molecular mass of phosphorylated paxillin on SDS-PAGE immunoblots of NGF treated rat PC12 cells, as well as calyculin A treated human A431 cells and mouse macrophages.
Phosphorylated
Ser-83
Western blots performed on each lot.
For research use only. Not intended for therapeutic or diagnostic use. Use of all products is subject to our terms and conditions, which can be viewed on our website.
United States
After date of receipt, stable for at least 1 year at -20°C.
Blue Ice
UniProt (Immunogen Species): Q8VI36
Product Specific References for Applications and Species
Fan, Y, et al. 2018. Differential Regulation of Adhesion and Phagocytosis of Resting and Activated Microglia by Dopamine. Frontiers in cellular neuroscience, 309.
Fusté, N.P., et al. 2016. Cytoplasmic cyclin D1 regulates cell invasion and metastasis through the phosphorylation of paxillin. Nature Communications, 11581.
Fusté, N.P., et al. 2016. Cytoplasmic cyclin D1 regulates cell invasion and metastasis through the phosphorylation of paxillin. Nature Communications, 11581.
Fan, Y, et al. 2021. Salidroside Improves Chronic Stress Induced Depressive Symptoms Through Microglial Activation Suppression. Frontiers in pharmacology, 635762.
Fan, Y, et al. 2018. Differential Regulation of Adhesion and Phagocytosis of Resting and Activated Microglia by Dopamine. Frontiers in cellular neuroscience, 309.
Fusté, N.P., et al. 2016. Cytoplasmic cyclin D1 regulates cell invasion and metastasis through the phosphorylation of paxillin. Nature Communications, 11581.
Veith, C., et al. 2014. Hypoxia- or PDGF-BB-dependent paxillin tyrosine phosphorylation in pulmonary hypertension is reversed by HIF-1α depletion or imatinib treatment. Thrombosis and Haemostasis, 1288-1303.
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