Our Paxillin (Tyr-118) rabbit polyclonal phosphospecific primary antibody from PhosphoSolutions is produced in-house. It detects chicken, human, mouse, rat, and Xenopus Paxillin (Tyr-118) and is antigen affinity purified. It is great for use in WB.
Western blot analysis of A431 cells (30 µg/lane) serum starved overnight (lane 1 & 3) or treated with pervanadate (1 mM) for 30 min (lanes 2 & 4). The blot was probed with mouse monoclonal anti-Paxillin (PM1071) (lanes 1 & 2) and rabbit polyclonal anti-phospho-Paxillin (Tyr-118) (PP4501) (lanes 3 & 4).
Paxillin is involved in focal adhesion formation during cell adhesion and migration. Paxillin contains LD motifs, LIM domains, and an SH3- and SH2-binding domain that participate in a variety of protein-protein interactions with kinases, GTPase-activating proteins, and cytoskeletal proteins. Phosphorylation of paxillin occurs at both tyrosine and serine sites. Tyrosine phosphorylation of paxillin occurs in response to growth factors, neuropeptides, and integrins. The major sites of tyrosine phosphorylation include Tyr-31 and Tyr-118. Both of these sites may be involved in Crk binding to paxillin during integrin-mediated cell adhesion. These sites may provide docking motifs for recruitment of other signaling molecules to focal adhesions.
Antigen Affinity Purified
Polyclonal
IgG
WB
Rabbit
PXN
68
Phospho-Paxillin (Tyr-118) synthetic peptide (coupled to KLH) corresponding to amino acid residues around tyrosine 118 from human paxillin. This sequence is highly conserved in rat and mouse paxillin and is also found in all isoforms (a, b, g) of paxillin.
Chicken, Frog, Human, Mouse, Rat
Storage at -20°C is recommended, as aliquots may be taken without freeze/thawing due to presence of 50% glycerol. Stable for at least 1 year at -20°C.
Liquid
PBS + 1 mg/ml BSA, 0.05% NaN3 and 50% glycerol
WB: 1:1000
Unconjugated
This antibody detects a 68kDa* protein corresponding to the molecular mass of paxillin on SDS-PAGE immunoblots of A431 cells treated with pervanadate. This reactivity is not observed after akaline phosphatase treatment.
Phosphorylated
Tyr 118
Western blots performed on each lot.
For research use only. Not intended for therapeutic or diagnostic use. Use of all products is subject to our terms and conditions, which can be viewed on our website.
United States
After date of receipt, stable for at least 1 year at -20°C.
Juanes, MA, et al. 2019. The role of APC-mediated actin assembly in microtubule capture and focal adhesion turnover. The Journal of cell biology, 3415-3435.
Chang, TY, et al. 2017. Paxillin facilitates timely neurite initiation on soft-substrate environments by interacting with the endocytic machinery. eLife, e31101.
Tachibana, H, et al. 2023. Plasma Membrane of Focal Adhesions has a High Content of Cholesterol and Phosphatidylcholine with Saturated Acyl Chains. Journal of cell science, jcs260763.
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