Our Semaphorin-3A (Central region) rabbit polyclonal primary antibody from PhosphoSolutions is produced in-house. It detects human, mouse, and rat Semaphorin-3A (Central region) and is antigen affinity purified. It is great for use in WB, ICC, IHC.
Western blots of neonatal rat brain (lanes 1, 3 & 5) and human recombinant Sema3A/Fc chimera (95/125 kDa) (lanes 2, 4 & 6). Blots were probed with anti-Sema3A (SP1401) (lanes 1 & 2), anti-Sema3A (SP1221) (lanes 3 & 4) and anti-Sema3A (SP1241) (lane 5 & 6). The antibodies recognize both the 95 kDa and 125 kDa forms of the recombinant Sema3A.
One family of inhibitory axon guidance molecules is the semaphorins. The semaphorins include secreted, transmembrane, and GPI-anchored extracellular molecules that are involved in regulating axon guidance by inhibiting axons from growing toward incorrect targets. Semaphorin 3A (Sema3A) may play a particularly interesting role in limiting axon regeneration since it is expressed in meningeal fibroblasts that invade the injured spinal cord and surround the glial scar. In addition, the Sema3A co-receptors, Neuropilin-1 and Plexin-A1, are expressed on axons that regenerate up to the injured region, but do not cross this Sema3A-containing region. Thus, Sema3A and its co-receptors may have important roles in regulating axon guidance during neuronal development and after neuronal injury.
Antigen Affinity Purified
Polyclonal
IgG
ICC, IHC, WB
Rabbit
SEMA3A
95
Semaphorin 3A synthetic peptide (coupled to carrier protein) corresponding to amino acids in the central region of human Sema3A. The sequence used is highly conserved in rat and mouse Sema3A, and has low homology to other semaphorin family members.
Human, Mouse, Rat
Storage at -20°C is recommended, as aliquots may be taken without freeze/thawing due to presence of 50% glycerol. Stable for at least 1 year at -20°C.
Liquid
PBS + 1 mg/ml BSA, 0.05% NaN3 and 50% glycerol
WB: 1:1000
IHC: 6:00
ICC: 1:100
Unconjugated
The antibody detects a 95kDa* protein corresponding to the apparent molecular mass of Sema3A on SDS-PAGE immunoblots of human recombinant Sema3A and neonatal rat brain lysates.
Western blots performed on each lot.
For research use only. Not intended for therapeutic or diagnostic use. Use of all products is subject to our terms and conditions, which can be viewed on our website.
United States
After date of receipt, stable for at least 1 year at -20°C.
