PhosphoSolutions’ Antibodies that Work™ have been made and tested right here in Denver, Colorado since 2001. With a focus in both neuroscience and cancer research, we pride ourselves on offering the highest quality antibodies and customer support in the business. Our serum-pooling initiative ensures reproducible polyclonal antibodies that come with a 100% satisfaction guarantee. Our all-inclusive custom antibody services allow you to work directly with the scientist developing your antibody: from design and immunization to purification and characterization. We understand how frustrating it can be to conduct research using ineffective antibodies, and we believe that you shouldn’t have to.
![Western blot of mouse kidney lysate showing specific immunolabeling of the ~160 kDa NCC protein phosphorylated at Thr53 in the first lane (-). Phosphospecificity is shown in the second lane (+) where immunolabeling is completely eliminated by blot treatment with lambda phosphatase (λ-Ptase, 1200 units for 30 min).](http://www.antibodiesinc.com/cdn/shop/files/NCC-T53-WB-CNC-e1491943713654_bb141718-1241-4f01-be2b-fa8fcd9b6863_{width}x.jpg?v=1692737239)
![Immunostaining of PFA perfused frozen kidney sections from WT and NCC KO mice showing specific labeling of the NCC protein phosphorylated at Thr53 (cat. p1311-53, red, 1:100,000) on the left and the absence of staining in the KO on the right. (Image courtesy of Lauren Miller, Ellison Lab, OHSU.)](http://www.antibodiesinc.com/cdn/shop/files/p1311-53-if-1_{width}x.jpg?v=1692737240)