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Western blot analysis of draxin expression in rat PC12 cells (lane 1), rat P1 brain (lane 2), adult mouse brain (lane 3), and chick E9 brain (lane 4). The blot was probed with rabbit polyclonal anti-Draxin (C-terminal region) at 1:1000.Immunocytochemical labeling of Draxin in rat PC12 cells differentiated with NGF. The cells were probed with Draxin (C-terminal region) rabbit polyclonal antibody, then the antibody was detected using appropriate secondary antibody conjugated to Cy3. The antibody was used in the absence (left) or presence (right) of blocking peptide (DX3675). Lower images show corresponding phase images.
PhosphoSolutions Anti-Draxin (C-terminal region) Antibody
$285
Western blot image of Jurkat cells stimulated with calyculin A (100 nM, 30 min) (lanes 1-6) followed by lambda phosphatase (lanes 2 & 6) or alkaline phosphatase (lane 4) treatment. The blots were probed with anti-Dok1 (lanes 1 & 2), anti-Dok1 (Tyr-362) (lanes 3 & 4), and anti-Dok1 (Ser-450) (lanes 5 & 6).
PhosphoSolutions Anti-Dok1 (Ser-450), Phosphospecific Antibody
$340
Western blot image of Jurkat cells stimulated with calyculin A (100 nM, 30 min) (lanes 1-6) followed by lambda phosphatase (lanes 2 & 6) or alkaline phosphatase (lane 4) treatment. The blots were probed with anti-Dok1 (lanes 1 & 2), anti-Dok1 (Tyr-362) (lanes 3 & 4), and anti-Dok1 (Ser-450) (lanes 5 & 6).
PhosphoSolutions Anti-Dok1 (Tyr-362) [Dok2 (Tyr-337)], Phosphospecific Antibody
$340
Western blot of DISC1 phosphorylation in PC12 cells untreated (lanes 1 & 3) or treated with calyculin A (100 nm) for 30 min (lanes 2 & 4). The blots were probed with DP3041 anti-DISC1 (a.a. 740-753) (lanes 1 & 2) or DP3061 anti-DISC1 (Ser-710) (lanes 3 & 4).
PhosphoSolutions Anti-DISC1 (Ser-710), Phosphospecific Antibody
$340
Western blot of DISC1 in mouse brain (lanes 1 & 3) and rat PC12 cells (lanes 2 & 4). The blots were probed with DP3021 anti-DISC1 (a.a. 740-753) (lanes 1 & 2) and DP3041 anti-DISC1 (a.a. 740-753) (lanes 3 & 4).
PhosphoSolutions Anti-DISC1 Antibody
$285
Western blot of DISC1 in mouse brain (lanes 1 & 3) and rat PC12 cells (lanes 2 & 4). The blots were probed with DP3021 anti-DISC1 (a.a. 740-753) (lanes 1 & 2) and DP3041 anti-DISC1 (a.a. 740-753) (lanes 3 & 4).
PhosphoSolutions Anti-DISC1 (C-terminus) Antibody
$285
Western blot analysis of mDia3 expression in human Jurkat cells treated with Calyculin A (100 nM) (lanes 1-4). The blots were treated with lambda phosphatase (lanes 2 & 4), then probed with rabbit polyclonal anti-mDia3 (C-terminus; DP4511) (lanes 1 & 2) and anti-phospho-mDia3 (Ser-196; DP4521) (lanes 3 & 4).
PhosphoSolutions Anti-mDia3 (Ser-196)) [conserved site], Phosphospecific Antibody
$340
Western blot analysis of mDia3 expression in human Jurkat cells treated with Calyculin A (100 nM) (lanes 1-4). The blots were treated with lambda phosphatase (lanes 2 & 4), then probed with rabbit polyclonal anti-mDia3 (C-terminus; DP4511) (lanes 1 & 2) and anti-phospho-mDia3 (Ser-196; DP4521) (lanes 3 & 4).
PhosphoSolutions Anti-mDia3 (C-terminus) Antibody
$340
Western blot analysis of COS-7 cells expressing flag-tagged mDia2 (lanes 1-3) or mDia2 mutant (T1061A) (lane 4). The cells were untreated (lane 1) or treated with calyculin A (lanes 2 & 3). The blot was treated with alkaline phosphatase (lane 2), then probed with anti-mDia2 (Thr-1061) (upper blot) or anti-flag antibody (lower blot). (Image provided by Dr. Christopher Mack in the Dept. of Pathology at UNC Chapel Hill).
PhosphoSolutions Anti-mDia2 (Thr-1061), Phosphospecific Antibody
$340
Western blot analysis of mDia2 expression in rat PC12 (lane 1), human A431 (lane 2), mouse brain (lane 3), and rabbit spleen fibroblasts (lane 4). The blots were probed with anti-mDia2 (C-terminal region) at 1:500.
PhosphoSolutions Anti-mDia2 (C-terminal region) Antibody
$340
Western blot analysis of mDia1 expression in human Jurkat cells (lanes 1-4). The blots were probed with anti-mDia1 (a.a. 66-77: DP4471) in the presence (lane 1) or absence of blocking peptide (DX4475) using dilutions of 1:250 (lane 2), 1:1000 (lane 3), and 1:4000 (lane 4).
PhosphoSolutions Anti-mDia1 (N-terminal region) Antibody
$340
Western blot analysis of DAAM1 expression in mouse C2C12 (lane 1), human A431 (lane 2), and K562 (lane 3) cell lysates. The blots were probed with mouse monoclonal DAAM1 (N-terminal region) antibody at 1:500.Immunocytochemical labeling of DAAM1 in aldehyde-fixed and NP-40-permeabilized A431 cells. The cells were labeled with mouse monoclonal DAAM1 (N-terminal region) antibody, then the antibody was detected using appropriate secondary antibody conjugated to DyLight® 594. The corresponding phase image is shown to the right.
PhosphoSolutions Anti-DAAM1 (N-terminal region) Antibody
$285
Western blot analysis of human LNCaP (lane 1), MCF7 (lane 2), MDA-MB-231 (lane 3), and MeWo (lane 4) cell lysates, as well as human recombinant full-length cyclophilin B (lane 5) and cyclophilin A (lane 6). The blot was probed with mouse monoclonal anti-cyclophilin B (CM0191) at 1:500.Immunocytochemical labeling of cyclophilin B in methanol:acetone (1:1) fixed human A549 lung cancer cells. The cells were labeled with mouse monoclonal anti-cyclophilin B (CM0191). The antibody was detected using goat anti-mouse DyLight® 594.
PhosphoSolutions Anti-Cyclophilin B Antibody
$370
Western blot analysis of human Jurkat cells treated with 100 nM calyculin A for 30 min. then the blots were untreated (-) or treated (+) with lambda phosphatase. The blots were probed with rabbit polyclonals anti-CXCR4 (Ser-324/Ser-325) (left panel) or anti-CXCR4 (a.a. 322-329) (right panel).Immunocytochemical labeling of CXCR4 in chick pluripotent cells. The cells were labeled with rabbit polyclonal CXCR4 (S324/S325) antibody (CP4251), then detected using appropriate secondary antibody (Red). (Image provided by Dr. Yangqing Lu at the Regenerative Bioscience Center, University of Georgia).
PhosphoSolutions Anti-CXCR4 (Ser-324/Ser-325), Phosphospecific Antibody
$340
Western blot analysis of human Jurkat cells treated with 100 nM calyculin A for 30 min. then the blots were untreated (-) or treated (+) with lambda phosphatase. The blots were probed with rabbit polyclonal anti-CXCR4 (Ser-324/Ser-325) (left panel), or anti-CXCR4 (a.a. 328-338) (right panel).Immunocytochemical labeling of CXCR4 in chick pluripotent cells. The cells were labeled with rabbit polyclonal CXCR4 (a.a. 328-338) antibody (CP4231), then detected using appropriate secondary antibody (Red). (Image provided by Dr. Yangqing Lu at the Regenerative Bioscience Center, University of Georgia).
PhosphoSolutions Anti-CXCR4 (C-terminal region) Antibody
$285
Western blot analysis of human Jurkat cells treated with 100 nM calyculin A for 30 min. then the blots were untreated (-) or treated (+) with lambda phosphatase. The blots were probed with rabbit polyclonals anti-CXCR4 (Ser-324/Ser-325) (left panel) or anti-CXCR4 (a.a. 322-329) (right panel).Immunocytochemical labeling of CXCR4 in chick pluripotent cells. The cells were labeled with rabbit polyclonal CXCR4 (a.a. 322-329) antibody (CP4211), then detected using appropriate secondary antibody (Red). (Image provided by Dr. Yangqing Lu at the Regenerative Bioscience Center, University of Georgia).
PhosphoSolutions Anti-CXCR4 (C-terminal region) Antibody
$285
Western blot of rat PC12 cells stimulated with calyculin A (100 nM) for 30 min (lanes 1-4). Then the blot was treated with lambda phosphatase to dephosphorylate CRMP2 (lanes 2 & 4). The blot was probed with rabbit polyclonal anti-CRMP2 (C-terminal Region) antibody (lanes 1 & 2) or mouse monoclonal anti-CRMP2 (Thr-555) antibody (lanes 3 & 4).
PhosphoSolutions Anti-CRMP2 (Thr-555), Phosphospecific Antibody
$340
Western blot of rat PC12 cells untreated (lanes 1 & 3) or treated with calyculin A (lanes 2 & 4). The blot was probed with anti-CRMP2 (C-terminal Region) antibody (lanes 1 & 2) or anti-CRMP2 (Thr-555) antibody (lanes 3 & 4).Immunocytochemical labeling of phosphorylated CRMP2 in mouse C2C12 cells. The cells were probed with CRMP2 (C-terminal region) and CRMP2 (Thr-555) rabbit polyclonal antibodies, then the antibodies were detected using appropriate secondary antibodies conjugated to Cy3. The antibodies were used in the absence (left) or presence (right) of their respective blocking peptide (CX2165 or CX2255).
PhosphoSolutions Anti-CRMP2 (Thr-555), Phosphospecific Antibody
$340
Western blot image of mouse brain untreated (lanes 1 & 3) or treated with lambda phosphatase (lanes 2 & 4). The blot was probed with anti-CRMP2 (C-terminal Region) (lanes 1 & 2) or anti-CRMP2 (Ser-522) (lanes 3 & 4).Immunocytochemical labeling of CRMP2 phosphorylation in aldehyde-fixed and NP-40-permeabilized NGF-differentiated PC12 cells. The cells were labeled with rabbit polyclonal anti-CRMP2 (Ser-522) (CP2191) antibody in the absence (Left) or presence (Right) of blocking peptide (CX2195). The antibody was detected using appropriate secondary antibody conjugated to DyLight® 594.
PhosphoSolutions Anti-CRMP2 (Ser-522), Phosphospecific Antibody
$340
Western blot image of mouse brain untreated (lanes 1 & 3) or treated with lambda phosphatase (lanes 2 & 4). The blot was probed with anti-CRMP2 (C-terminal Region) (lanes 1 & 2) or anti-CRMP2 (Ser-522) (lanes 3 & 4).Immunocytochemical labeling of phosphorylated CRMP2 in mouse C2C12 cells. The cells were probed with CRMP2 (C-terminal region) and CRMP2 (Thr-555) rabbit polyclonal antibodies, then the antibodies were detected using appropriate secondary antibodies conjugated to Cy3. The antibodies were used in the absence (left) or presence (right) of their respective blocking peptide (CX2165 or CX2255).
PhosphoSolutions Anti-CRMP2 (C-terminal region) Antibody
$340
Western blot of human K562 cells stimulated with pervanadate (1 mM) for 30 min. (lanes 1-4). The blot was treated with alkaline phosphatase to dephosphorylate CrkL (lanes 2 & 4), then the blot was probed with rabbit polyclonals CrkL (C-terminus) CP3081 (lanes 1 & 2) and CrkL (Tyr-207) phospho-specific CP4671 (lanes 3 & 4).
PhosphoSolutions Anti-CrkL (Tyr-207), Phosphospecific Antibody
$340
Western blot of human K562 cells stimulated with pervanadate (1 mM) for 30 min. (lanes 1-4). The blot was treated with alkaline phosphatase to dephosphorylate CrkL (lanes 2 & 4), then the blot was probed with rabbit polyclonals CrkL (C-terminus) CP3081 (lanes 1 & 2) and CrkL (Tyr-207) phospho-specific CP4671 (lanes 3 & 4).
PhosphoSolutions Anti-CrkL (C-terminus) Antibody
$285
Western blot of human K562 cells stimulated with pervanadate (1 mM) for 30 min. (lanes 1-6). The blot was treated with akaline phosphatase to dephosphorylate Crk (lanes 2, 4 & 6), then the blot was probed with rabbit polyclonals CrkL (C-terminus) CP3081 (lanes 1 & 2), Crk (Tyr-251) phospho-specific CP3091 (lanes 3 & 4), and mouse monoclonal anti-Crk II (C-terminal region) (lanes 5 & 6).
PhosphoSolutions Anti-Crk II (Tyr-251), Phosphospecific Antibody
$340
Western blot of human K562 cells stimulated with pervanadate (1 mM) for 30 min. (lanes 1-4). The blot was treated with alkaline phosphatase to dephosphorylate Crk II (lanes 2 & 4), then the blot was probed with mouse monoclonal Crk II (C-terminal region) CM3321 (lanes 1 & 2) and Crk II (Tyr-221) phospho-specific CP4701 (lanes 3 & 4).
PhosphoSolutions Anti-Crk II (Tyr-221), Phosphospecific Antibody
$340

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