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Western blot analysis of mouse J774A.1 macrophage stimulated with calyculin A (lanes 1-4) then dephosphorylated with lambda phosphatase (lanes 2 & 4). The blots were probed with mouse monoclonal anti-Bad (lanes 1 & 2), and rabbit polyclonal anti-Bad (Ser-112) (lanes 3 & 4).
PhosphoSolutions Anti-Bad (Ser-112), Phosphospecific Antibody
$340
Western blot analysis of mouse J774A.1 macrophage stimulated with calyculin A (lanes 1-4) then dephosphorylated with lambda phosphatase (lanes 2 & 4). The blots were probed with mouse monoclonal anti-Bad (lanes 1 & 2), and rabbit polyclonal anti-Bad (Ser-26) (lanes 3 & 4).
PhosphoSolutions Anti-Bad (Ser-26), Phosphospecific Antibody
$340
Western blot of human LNCaP prostate adenocarcinoma (lane 1), MCF-7 breast ductal carcinoma (lane 2), NCI-H28 pleural mesothelioma (lane 3), A431 epidermoid carcinoma (lane 4), MDAMB-231 breast carcinoma (lane 5) and Jurkat T-cell line (lane 6). The blot was probed with mouse monoclonal anti-B7-H3/CD276 (BM0451) at 1:500.Immunocytochemical labeling of B7-H3 in aldehyde fixed human MCF-7 breast ductal carcinoma cells. The cells were labeled with mouse monoclonal anti-B7-H3/CD276 (BM0451). The antibody was detected using goat anti-mouse DyLight® 594.
PhosphoSolutions Anti-B7-H3/CD276 (Extracellular region) Antibody
$370
Western blot of human MDA-MB-231 breast carcinoma (lane 1), PC-3 prostate adenocarcinoma (lane 2), A549 lung carcinoma (lane 3), NCI-H1915 lung carcinoma (lane 4), and A431 epidermoid carcinoma (lane 5). The blot was probed with mouse monoclonal anti-Axl (AM0471) at 1:1000.Immunocytochemical labeling of Axl in aldehyde fixed human MDAMB-231 breast carcinoma cells. The cells were labeled with mouse monoclonal anti-Axl (AM0471). The antibody was detected using goat anti-mouse DyLight® 594.
PhosphoSolutions Anti-Axl/UFO (Extracellular region) Antibody
$370
Western blot image of human recombinant Atrogin 1 (lanes 1-6). The blot was probed with rabbit polyclonal Atrogin-1 (lanes 1-3) and rat monoclonal Atrogin-1 (lanes 4-6) at 1:1000 (lanes 1 & 4), 1:2000 (lanes 2 & 5), and 1:4000 (lanes 3 & 6).
PhosphoSolutions Anti-Atrogin-1 Antibody
$340
Western blot of human A431 cells treated with Calyculin A (100 nM) for 30 min. Blot lanes were untreated (lanes 1, 3, & 5) or treated with lambda phosphatase (lanes 2, 4, & 6) then probed with anti-ATM (Ser-794) (lanes 1 & 2), anti-ATM (C-Terminal) (lanes 3 & 4), or anti-ATM (Ser-1981) (lanes 5 & 6).Immunocytochemical labeling of ATM phosphorylation in control (Top row) or calyculin A-treated A431 cells (Bottom row). The cells were labeled with mouse monoclonal ATM (C-terminal region) (AM3611) and ATM (Ser-1981) (AM3661). The antibodies were detected using goat anti-mouse-DyLight® 594.
PhosphoSolutions Anti-ATM (Ser-1981), Phosphospecific Antibody
$340
Immunocytochemical labeling of ATM phosphorylation in calyculin A-treated A431 cells. The cells were labeled with rabbit polyclonal anti-ATM (Ser-794) (AP3631) antibody in the absence (Left) or presence (Right) of blocking peptide (AX3635). The antibody was detected using appropriate secondary antibody conjugated to DyLight® 594.
PhosphoSolutions Anti-ATM (Ser-794), Phosphospecific Antibody
$340
Western blot of human A431 cells treated with Calyculin A (100 nM) for 30 min. Blot lanes were untreated (lanes 1, 3, & 5) or treated with lambda phosphatase (lanes 2, 4, & 6) then probed with anti-ATM (Ser-794) (lanes 1 & 2), anti-ATM (C-Terminal) (lanes 3 & 4), or anti-ATM (Ser-1981) (lanes 5 & 6).Immunocytochemical labeling of ATM phosphorylation in control (Top row) or calyculin A-treated A431 cells (Bottom row). The cells were labeled with mouse monoclonal ATM (C-terminal region) (AM3611) and ATM (Ser-1981) (AM3661). The antibodies were detected using goat anti-mouse-DyLight® 594.
PhosphoSolutions Anti-ATM (C-terminal region) Antibody
$285
Western blot analysis of mouse macrophage J774A.1 cells stimulated with pervanadate (1 mM for 30 min.), then untreated (-) or treated (+) with alkaline phosphatase. The blot was probed with rabbit polyclonal anti-Asc (Tyr-144) phospho-specific antibody (AP5631) at 1:500.Immunocytochemical labeling of Asc (Tyr-144) in inflammasomes. Paraformaldehyde fixed J774 cells were primed with LPS and treated with nigericin. Cells were co-labeled with DAPI, a caspase-1 inhibitor (FAM-YVAD-FMK), and anti-Asc (Tyr-144) phosphospecific antibody detected with AlexaFluor 568 secondary. (Image provided by Jordan Yaron, Center for Biosignatures Discovery Automation, Arizona State University)
PhosphoSolutions Anti-Asc (Tyr-144), Phosphospecific Antibody
$340
Western blot analysis of Arpc1b phosphorylation in human A431 stimulated with calyculin A (100 nM) for 30 min (lanes 1 & 3). The blots were treated with lambda phosphatase to remove phosphorylation (lanes 2 & 4), then probed with anti-Arpc1b (C-terminal region) (lanes 1 & 2) or anti-Arpc1b (Thr-21) (lanes 3 & 4).
PhosphoSolutions Anti-Arpc1b/p41-Arc (Thr-21), Phosphospecific Antibody
$340
Western blot of rat A7r5 (lane 1), human Jurkat (lane 2), rat PC12 (lane 3), human PC3 (lane 4), mouse C2C12 (lane 5), and human A431 cells (lane 6). The blot was probed with rabbit polyclonal anti-Arpc1b (C-terminal region).
PhosphoSolutions Anti-Arpc1b/p41-Arc (C-terminal region) Antibody
$340
Western blot of human A431 (lane 1), Jurkat (lane 2), and HeLa (lane 3) cells. The blots were probed with rabbit polyclonal anti-Arp3 (C-terminal region) antibody at 1:1000 (lanes 1-3).Immunocytochemical labeling of Arp3 in aldehyde-fixed and NP-40-permeabilized rat PC12 cells differentiated with NGF. The cells were labeled with rabbit polyclonal anti-Arp3 (C-terminal region) (AP4581) in the absence (left) or presence (right) of blocking peptide (AX4585). The antibody was detected using appropriate secondary antibody conjugated to DyLight® 594.
PhosphoSolutions Anti-Arp3 (C-terminal region) Antibody
$285
Western blot of human A431 cells treated with Calyculin A (100 nM) for 30 min. Blot lanes were untreated (lanes 1 & 3) or treated with lambda phosphatase (lanes 2 & 4) then probed with anti-Arp2 (C-terminal) (lanes 1 & 2) or anti-Arp2 (Thr-237/Thr-238) (lanes 3 & 4).Immunocytochemical labeling of Arp2 phosphorylation in rat PC12 cells differentiated with NGF. The cells were probed with Arp2 (C-terminal region) and Arp2 (Thr-237/Thr-238) rabbit polyclonal antibodies, then the antibodies were detected using appropriate secondary antibody conjugated to Cy3.
PhosphoSolutions Anti-Arp2 (Thr-237/Thr-238), Phosphospecific Antibody
$340
Western blot of human A431 cells treated with Calyculin A (100 nM) for 30 min. Blot lanes were untreated (lanes 1 & 3) or treated with lambda phosphatase (lanes 2 & 4) then probed with anti-Arp2 (C-terminal) (lanes 1 & 2) or anti-Arp2 (Thr-237/Thr-238) (lanes 3 & 4).Immunocytochemical labeling of Arp2 phosphorylation in rat PC12 cells differentiated with NGF. The cells were probed with Arp2 (C-terminal region) and Arp2 (Thr-237/Thr-238) rabbit polyclonal antibodies, then the antibodies were detected using appropriate secondary antibody conjugated to Cy3.
PhosphoSolutions Anti-Arp2 (C-terminal region) Antibody
$340
Western blot analysis of human A431 cells treated with EGF (100 ng/ml for 60 min.) (lanes 1-4). The blot was treated with lambda phosphatase (lanes 2 & 4) then probed with rat monoclonal anti-Ago2 (AM5271) (lanes 1 & 2) and rabbit polyclonal anti-Ago2 (Tyr-393) phospho-specific antibody (lanes 3 & 4).
PhosphoSolutions Anti-Argonaute 2 (Tyr-393), Phosphospecific Antibody
$340
Western blot analysis of mouse recombinant Ago2 full length protein (lanes 1-4). The blot was treated with lambda phosphatase (lanes 2 & 4) then probed with rabbit polyclonal anti-Ago2 (AP5281) (lanes 1 & 2) and rabbit polyclonal anti-Ago2 (Ser-387) phospho-specific antibody (lanes 3 & 4).
PhosphoSolutions Anti-Argonaute 2 (Ser-387), Phosphospecific Antibody
$340
Western blot analysis of mouse recombinant Ago2 full length protein (lanes 1-4). The blot was treated with lambda phosphatase (lanes 2 & 4) then probed with rabbit polyclonal anti-Ago2 (AP5281) (lanes 1 & 2) and rabbit polyclonal anti-Ago2 (Ser-387) phospho-specific antibody (lanes 3 & 4).
PhosphoSolutions Anti-Argonaute 2 Antibody
$285
Western blot analysis of human A431 cells treated with EGF (100 ng/ml for 60 min.) (lanes 1-4). The blot was treated with lambda phosphatase (lanes 2 & 4) then probed with rat monoclonal anti-Ago2 (AM5271) (lanes 1 & 2) and rabbit polyclonal anti-Ago2 (Tyr-393) phospho-specific antibody (lanes 3 & 4).
PhosphoSolutions Anti-Argonaute 2 (N-terminus) Antibody
$285
PhosphoSolutions Anti-APC (Central region) Antibody
$285
PhosphoSolutions Anti-APC (C-terminal region) Antibody
$285
Western blot of human PC3 cells (lane 1), human breast tissue (lane 2), lung tissue (lane 3), skin tissue (lane 4), and brain tissue (lane 5). The blot was probed with mouse monoclonal anti-Annexin A5 antibody (AM0171) at 1:500 (lanes 1-5).Western blot of A431 cell lysate only (lane 1), AM0171 antibody only (lane 2), and AM0171 antibody immunoprecipitate from A431(lane 3). The blot was probed with mouse monoclonal anti-Annexin A5 antibody (AM0171) at 1:500 (lanes 1-3). The asterisk shows the antibody heavy chain in immunopreciptates at 50 kDa, while Annexin A5 band is observed at 35 kDa.
PhosphoSolutions Anti-Annexin A5 Antibody
$371
Western blot of rat PC12 cell lysate. The blot was probed with mouse monoclonal anti-Annexin A2 antibody at 1:250 (lane 1) or 1:1000 (lane 2).Immunocytochemical labeling of Annexin A2 in NGF-differentiated rat PC12 cells. The cells were fixed in paraformaldehyde and permeabilized using NP-40, then labeled with mouse monoclonal Annexin A2 (C-terminal region). The antibody was detected using goat anti-mouse DyLight® 594.
PhosphoSolutions Anti-Annexin A2 (C-terminal region) Antibody
$371
Western blot of human A431 (lane 1), MDA-MB-231 (lane 2), LNCaP (lane 3), MeWo (lane 4), HUVEC (lane 5), Jurkat (lane 6), K562 (lane 7), and PC3 (lane 8) cell lysates. The blot was probed with mouse monoclonal anti-Annexin A2 antibody (AM0091) at 1:500 (lanes 1-8).Immunocytochemical labeling of Annexin A2 in paraformaldehyde fixed human A431 cells. The cells were labeled with mouse monoclonal anti-Annexin A2 (clone M009). The antibody was detected using goat anti-mouse DyLight® 594.
PhosphoSolutions Anti-Annexin A2 Antibody
$371
Western blot of human A431 cells (lane 1), A549 cells (lane 2), LNCaP cells (lane 3), full-length recombinant human annexin A1 (lane 4), and recombinant human annexin A2 (lane 5). The blot was probed with mouse monoclonal anti-Annexin A1 antibody (AM0211) at 1:1000 (lanes 1-5).Immunocytochemical labeling of Annexin A1 in paraformaldehyde fixed human A549 cells. The cells were labeled with mouse monoclonal anti-Annexin A1 (clone M021). The antibody was detected using goat anti-mouse Ig DyLight® 594.
PhosphoSolutions Anti-Annexin A1 Antibody
$371

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