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Western blot of rat hippocampal lysate showing specific immunolabeling of the ~67 kDa GAT-1 protein. Immunoflouresence of mouse retina specifically labeling GAT-1 protein (cat. 880-GAT1, red, 1:500) in the inner plexiform layer (IPL). The white identifies nuclei stained with DAPI. The retina was fixed with 4% paraformaldehyde for 30 minutes, embedded in 5% agarose, and sliced into 60 micron thick slices with a vibratome. The image is a Z-stack taken from a confocal microscope. Image courtesy of Andrea Wellington, Assistant Research Scientist, University of Arizona Physiology Dept.
PhosphoSolutions Anti-GABA Transporter (GAT) 1 Antibody
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Immunofluorescence of a section of rat hippocampus selectively labeling the neuronal cilia with anti-adenylate cyclase III (cat. 85-AC3, 1:10,000, red), labeling nuclei with anti-MECP2 (cat. 1205-MeCP2, 1:1000,green), and Hoechst staining of nuclear DNA.Immunostaining of cultured rat neurons and glia showing strong staining of neuronal cilia using our anti-adenylate cyclase III antibody (cat. 85-AC3, 1:500, red) and axonal and dendritic staining of alpha II spectrin (cat. 98-A2SM,1:500, green) revealing the submembranous cytoskeleton and DNA (blue).
PhosphoSolutions Anti-Adenylate Cyclase III Antibody
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Western blot of rat cortical lysate showing specific immunolabeling of the ~57 kDa α6-subunit of the GABAA-R.
PhosphoSolutions Anti-GABAA Receptor α6 Antibody
$375
Western blot of mouse forebrain lysates from Wild Type (WT) and α6-knockout (K/O) animals showing specific immunolabeling of the ~57 kDa α6-subunit of the GABAA-R. The labeling was absent from a lysate prepared from α6-knockout animals.
PhosphoSolutions Anti-GABAA Receptor α6 Antibody
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Western blot of mouse whole brain showing specific immunolabeling of the ~55 kDa α5-subunit of the GABAA-R.
PhosphoSolutions Anti-GABAA Receptor α5 Antibody
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Western blot of 10 µg of rat hippocampal lysate showing specific immunolabeling of the ~46 kDa γ2-subunit of the GABAA-R. Immunostaining of rat cerebellum showing labeling of GABAA γ2 subunit in fuchsia.
PhosphoSolutions Anti-GABAA Receptor γ2 Antibody
$375
Immunolabeling of mouse cortical cultures labeled with Anti-GFAP (cat. 620-GFAP, 1:1000, green) and anti-MAP2 (1100-MAP2, 1:1000, red).Western blot of rat cortical lysate showing specific immunolabeling of the ~50 kDa GFAP protein.
PhosphoSolutions Anti-Glial Fibrillary Acidic Protein (GFAP) Antibody
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High magnification confocal image of HeLa cells showing strong, prominent labeling of the nucleoli in the nucleus with Anti-Fibrillarin (cat. 560-FIB, 1:100, green), colabeled with Anti-Vimentin (cat. 2105-VIM, red, 1:1000) showing the cytoplasmic intermediated filaments, and nuclear staining was done with DAPI (blue).Western blot of HeLa cell lysate showing specific immunolabeling of the ~34 kDa fibrillarin protein.
PhosphoSolutions Anti-Fibrillarin Antibody
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Western blot of mouse adipose tissue lysate showing specific immunolabeling of the ~25 kDa DsbA-L protein.
PhosphoSolutions Anti-DsbA-L (Disulfide-bond-A Oxidoreductase-like Protein) Antibody
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Western blot of rat cerebellar lysate showing specific immunolabeling of the ~43 kDa connexin43 protein. Immunolabeling on astroglial in the CA1 and DG mouse hippocampus, and anterior cingulate cortex (ACC) specifically labeling Connexin43 (Cat no 327-CON, green, 1:200). Image from publication. CC-BY-4.0 PMID: 37239279
PhosphoSolutions Anti-Connexin 43 Antibody
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Immunofluorescence of HEK293 cells transfected with a fusion construct of GFP with the C-terminal region of S. aureas Cas9 protein (green) that is labeled with Anti-CRISPR Cas9 (cat. 304-Cas9, 1:1000, red)and the DNA stained blue with DAPI. The yellow label is the overlapping of the Anti-Cas9 antibody and the Cas9-GFP construct.Western blot of GFP-Cas9 transfected HEK293 cell lysate (+) showing specific immunolabeling of the ~53 kDa GFP-Cas9 fusion protein. Labeling is absent in the untransfected lysate (-).
PhosphoSolutions Anti-CRISPR Cas9 Antibody
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Western blot of rat cerebellar lysate showing specific immunolabeling of the ~22 kDa VSNL1 protein.Immunofluorescence of a section of rat cerebellum labeled with anti-visinin-like 1 protein (cat. 2145-VSNL1, 1:500, red) and anti-GFAP (cat. 620-GFAP, green, 1:5000). The anti-VSNL1 revels protein expressed in the granule cell menbranes and in synapses in the white matter and molecular layers. The anti-GFAP reveals protein expressed in the processes of Bergmann glia and astroglia.
PhosphoSolutions Anti-Visinin-Like Protein 1 (VSNL1) Antibody
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Western blot of 10 µg of HEK 293 cells expressing: 1) mGluR5 and 2) mGluR1a. Specific immunolabeling of the ~125 kDa monomer and the ~250 kDa dimer of mGluR1a is shown in the second lane (2). Specificity is confirmed in the first lane (1), as the mGluR1a antibody shows no reactivity toward mGluR5.
PhosphoSolutions Anti-Metabotropic Glutamate Receptor 1a Antibody
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Western blot of mouse whole brain lysate showing specific immunolabeling of the ~28 kDa TREM2 protein.Immunofluoresence of FLAG-tagged TREM2 transfected COS cells showing TREM2 (cat. 2006-TREM2, 1:150) staining in red and FLAG staining in green causing positive transfected cells to appear yellow. DNA is stained blue with DAPI.
PhosphoSolutions Anti-TREM2 Antibody
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Western blot of rat hippocampal lysate showing specific labeling of the ~38 kDa Synaptophysin2 protein. Immunostaining of 40DIV cultured rat cortical neurons showing punctate labeling of synaptophysin (cat. 1950-SNT2, red, 1:100). The blue is staining nuclear DNA. Cells and photo courtesy of QBMCellScience.
PhosphoSolutions Anti-Synaptophysin 2 Antibody
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Immunostaining of cultured newborn rat neurons and glia showing peripherin (cat. 1630-PER, green, 1:200) and neurofilament L (red). The nuclear DNA is stained blue.Western blot of rat cerebellar lysate showing specific immunolabeling of the ~57 kDa peripherin protein.
PhosphoSolutions Anti-Peripherin Antibody
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Western blot of neonatal rat brain lysate showing specific immunolabeling of the ~ 27 kDa Olig1 protein.Immunostaining of primary rat oligodendrocytes using anti-Olig1 (cat. 1537-OLIG1, red, 1:100).
PhosphoSolutions Anti-Olig1 Antibody
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Immunolabeling of E20 rat mixed neuron and glial cultured cells labeled with anti-neuron specific enolase antibody (cat. 1520-NSE, red, 1:500) and anti-GFAP antibody (cat. 621-GFAP, green, 1:5000). The blue is Hoechst stain of nuclear DNA. Anti-NSE labels protein expressed in neuronal cells and anti-GFAP labels protein expressed in intermediate filaments in astrocytic and other glial cells. Immunolabeling of HEK 293 cells labeled with anti-neuron specific enolase antibody (cat. 1520-NSE, red, 1:500) and anti-UCHL1 antibody (cat. 2060-UCHL1, green, 1:500).
PhosphoSolutions Anti-NSE (Neuron Specific Enolase) Antibody
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Western blot of 10 µg of HEK 293 cells specific immunolabeling of the ~120 kDa NR1 subunit of the NMDA receptor containing the C2’ splice variant insert (lanes 3 and 4). 1) No NR1 expression; 2) NR1 subunit containing only the C2 Insert; 3) NR1 subunit containing the C1 and C2' Insert; 4) NR1 subunit containing the N1 and C2' Insert.
PhosphoSolutions Anti-NMDA NR1 Subunit, Splice Variant C2' Antibody
$430
Western blot of 10 µg of HEK 293 cells showing specific immunolabeling of the ~120 kDa NR1 subunit of the NMDA receptor containing the C1 splice variant insert (in lane 3). 1) HEK cells without NR1 expression; 2) NR1 subunit containing only the C2 Insert; 3) NR1 subunit containing the C1 and C2' Insert; 4) NR1 subunit containing the N1 and C2' insert.
PhosphoSolutions Anti-NMDA NR1 Subunit, Splice Variant C1 Antibody
$430
Western blot of mouse habenula lysate showing specific immunolabeling of the ~52 kDa nAChRβ2 protein.
PhosphoSolutions Anti-Nicotinic Acetylcholine Receptor (nAChR) β2 Antibody
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Western blot of rat cortical lysate showing specific immunolabeling of the ~68 kDa NF-L protein.Immunofluorescence of a section of rat cerebellum showing specific labeling of Neurofilament-L (cat. 1453-NFL, 1:2000, green) and labeling of FOX3/NeuN (cat. 583-FOX3, 1:5000, red). The anti-NFL strongly labels the axons of basket calls and perikarya and processes of neuronal cells. The anti-FOX3/NeuN labels the nuclei and proximal cytoplasm of neurons. The blue is DAPI staining of nuclear DNA.
PhosphoSolutions Anti-Neurofilament NF-L Antibody
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Western blot of rat cortical lysate showing specific immunolabeling of the ~200 kDa NF-H protein.Immunofluorescence of a section of rat cerebellum showing specific labeling of Neurofilament H (cat. 1451-NFH, 1:25,000, red) in nuclei of neurons and specific labeling of GFAP (cat. 620-GFAP, 1:5000, green) in astrocytes and other glial cells, and DAPI staining of nuclear DNA.
PhosphoSolutions Anti-Neurofilament NF-H Antibody
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Western blot of rat hippocampal lysate showing specific immunolabeling of the ~115 kDa APP protein.
PhosphoSolutions Anti-Amyloid Precursor Protein Antibody
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