Anti-Dopamine Transporter (Thr53) Antibody
Our Anti-Dopamine Transporter (Thr53) rabbit polyclonal phosphospecific primary antibody from PhosphoSolutions is produced in-house. It detects rat Dopamine Transporter (Thr53) and is antigen affinity purified from pooled serum. It is great for use in WB.
Western blot of rat striatal lysate showing specific immunolabeling of the ~55 kDa glycosylated form of the DAT protein phosphorylated at Thr53 in the first lane (-). Phosphospecificity is shown in the second lane (+) where immunolabeling is blocked by preadsorption of the phosphopeptide used as the antigen, but not by the corresponding non-phosphopeptide (not shown).
Product Specific References for Applications and Species
| Western Blot: Mouse | ||
| PMID | Dilution | Publication |
| 33794225 | 1:2000 | Varman, D.R., et al. 2021. Novelty-induced Hyperactivity and Suppressed Cocaine Induced Locomotor Activation in Mice Lacking Threonine 53 Phosphorylation of Dopamine Transporter. Behavioral Brain Research,/i., p.113267. |
| 33599293 | not listed | Asami, M., et al. 2021. Dopamine and the phosphorylated dopamine transporter are increased in the diacylglycerol kinase ƞ‐knockout mouse brain. FEBS letters. |
| 33032296 | not listed | Alonso, I.P., et al. 2020. Dopamine transporter function fluctuates across sleep/wake state: potential impact for addiction. Neuropsychopharmacology, 46(4), pp.699-708. |
| 29739866 | not listed | Gowrishankar, R., et al. 2018. Region-specific regulation of presynaptic dopamine homeostasis by D2 autoreceptors shapes the in vivo impact of the neuropsychiatric disease-associated DAT variant Val559. Journal of Neuroscience, 38(23), pp.5302-5312. |
| 29486157 | not listed | Lu, Q., et al. 2018. Abnormalities of the serotonergic system in diacylglycerol kinase δ-deficient mouse brain. Biochemical and Biophysical Research Communications, 497(4), pp.1031-1037. |
| Western Blot: Rat | ||
| PMID | Dilution | Publication |
| 36301217 | 1:1000 | Samson, K.R., et al. 2022. Intermittent Access to Oxycodone Decreases Dopamine Uptake in the Nucleus Accumbens Core During Abstinence. Addiction Biology, e13241. |
| 30071195 | 1:1000 | Brodnik, Z.D., et al. 2018. Hypocretin receptor 1 involvement in cocaine-associated behavior: therapeutic potential and novel mechanistic insights. Brain Research, p.145894. |
| 26941626 | 1:5000 | Aher, Y.D., et al. 2016. A novel heterocyclic compound CE-104 enhances spatial working memory in the radial arm maze in rats and modulates the dopaminergic system. Frontiers in Behavioral Neuroscience, 10, p.20. |
| 26407764 | 1:5000 | Sase, A., et al. 2016. A heterocyclic compound CE-103 inhibits dopamine reuptake and modulates dopamine transporter and dopamine D1-D3 containing receptor complexes. Neuropharmacology, 102, pp.186-196. |
| 22722938 | not listed | Foster, J.D., et al. 2012. Dopamine transporter phosphorylation site threonine 53 regulates substrate reuptake and amphetamine-stimulated efflux. Journal of Biological Chemistry, 287(35), pp.29702-29712. |
Product Specific Protocols
- Western Blot Protocol: Download