Anti-NMDA NR2B Subunit (Tyr1472) Antibody
Our Anti-NMDA NR2B Subunit (Tyr1472) rabbit polyclonal phosphospecific primary antibody from PhosphoSolutions is produced in-house. It detects mouse and rat NMDA NR2B Subunit (Tyr1472) and is antigen affinity purified from pooled serum. It is great for use in WB.
Western blot of rat hippocampal lysate showing specific immunolabeling of the ~180 kDa NR2B subunit of the NMDAR phosphorylated at Tyr1472 in the first lane (-). Phosphospecificity is shown in the second lane (+) where immunolabeling is completely eliminated by lysate treatment with lambda phosphatase (400 units/100uL lysate for 30 min).
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Product Specific References for Applications and Species
| Immunocytochemistry: Rat | ||
| PMID | Dilution | Publication |
| 32651756 | 1:100 | Akkuratov, E.E., et al. 2020. Ouabain Modulates the Functional Interaction Between Na, K-ATPase and NMDA Receptor. Molecular Neurobiology, pp.1-13. |
| Western Blot: Human | ||
| PMID | Dilution | Publication |
| 31135041 | 1:1000 | Dedek, A., et al. 2019. Loss of STEP61 couples disinhibition to N-methyl-d-aspartate receptor potentiation in rodent and human spinal pain processing. Brain, 142(6), pp.1535-1546. |
| Western Blot: Mouse | ||
| PMID | Dilution | Publication |
| 39450669 | 1:800 | Escamilla, S, et al. 2024. Synaptic and extrasynaptic distribution of NMDA receptors in the cortex of Alzheimer's disease patients. Alzheimer's & Dementia, . |
| 37284938 | 1:500 | He, RB, et al. 2023. Ceftriaxone Improves Impairments in Synaptic Plasticity and Cognitive Behavior in APP/PS1 Mouse Model of Alzheimer's Disease by Inhibiting Extrasynaptic NMDAR-STEP61 Signaling. Journal of Neurochemistry, 215-232. |
| 34044147 | 1:1000 | Zhang, L., et al. 2021. Tyrosine phosphatase PTP1B impairs presynaptic NMDA receptor-mediated plasticity in a mouse model of Alzheimer's disease. Neurobiology of Disease, 156, p.105402. |
| 31543505 | 1:1000 | Ajit, D., et al. 2020. A unique tau conformation generated by an acetylation-mimic substitution modulates P301S-dependent tau pathology and hyperphosphorylation. Journal of Biological Chemistry, 294(45), pp.16698-16711. |
| 30518596 | 1:1000 | Salazar, S.V., et al. 2019. Alzheimer's disease risk factor Pyk2 mediates amyloid-β-induced synaptic dysfunction and loss. Journal of Neuroscience, 39(4), pp.758-772. |
| 31452242 | 1:1000 | Liu, G., et al. 2019. Loss of tau and Fyn reduces compensatory effects of MAP2 for tau and reveals a Fyn‐independent effect of tau on calcium. Journal of neuroscience research. Aug 26. |
| 30551522 | not listed | Zhang, X., et al. 2019. Effects of 5-Aza on p-Y1472 NR2B related to learning and memory in the mouse hippocampus. Biomedicine & Pharmacotherapy, 109, pp.701-707. |
| 27335408 | not listed | Xiao, X., et al. 2016. Disruption of Coordinated Presynaptic and Postsynaptic Maturation Underlies the Defects in Hippocampal Synapse Stability and Plasticity in Abl2/Arg-Deficient Mice. The Journal of Neuroscience, 36(25), 6778-6791. |
| 27094400 | 1:1000 | Zamzow, D.R., et al. 2016. Higher levels of phosphorylated Y1472 on GluN2B subunits in the frontal cortex of aged mice are associated with good spatial reference memory, but not cognitive flexibility. AGE, 38(3), 1-17. |
| 22544749 | 1:1000 | Xu, J., et al. 2012. Striatal-enriched protein-tyrosine phosphatase (STEP) regulates Pyk2 kinase activity. J Biol Chem. Jun 15;287(25):20942-56. |
| 22523092 | 1:500 | Gladding, C.M., et al. 2012. Calpain and Striatal-Enriched protein tyrosine phosphatase (STEP) activation contribute to extrasynaptic NMDA receptor localization in a Huntington’s disease mouse model. Hum Mol Genet. Sep 1;21(17):3739-52. |
| 21464302 | 1:1000 | Hinklin, T.R., et al. 2011. Alcohol inhibition of the NMDA receptor function, long-term potentiation, and fear learning requires striatal-enriched protein tyrosine phosphatase. PNAS, Apr 2011; 108: 6650 - 6655. |
| 20427654 | 1:1000 | Kurup, P., et al. 2010. Aβ-mediated NMDA receptor endocytosis in Alzheimer's disease involves ubiquitination of the tyrosine phosphatase STEP61. Journal of Neuroscience, 30(17), pp.5948-5957. |
| Western blot: Rat | ||
| PMID | Dilution | Publication |
| 34653560 | 1:1500 | Su, L., et al. 2021. . P2Y1 purinergic receptor inhibition attenuated remifentanil-induced postoperative hyperalgesia via decreasing NMDA receptor phosphorylation in dorsal root ganglion. Brain Research Bulletin, 177, pp.352-362. |
| 34166321 | 1:1000 | Su, L., et al. 2021. . P2Y1 Purinergic Receptor Contributes to Remifentanil-Induced Cold Hyperalgesia via Transient Receptor Potential Melastatin 8–Dependent Regulation of N-methyl-d-aspartate Receptor Phosphorylation in Dorsal Root Ganglion. Anesthesia & Analgesia, 133(3), pp.794-810. |
| 33257326 | not listed | Higginbotham, J.A., et al. 2021. . CB1 receptor signaling modulates amygdalar plasticity during context-cocaine memory reconsolidation to promote subsequent cocaine seeking. Journal of Neuroscience, 41(4), pp.613-629. |
| 32437889 | 1:500 | Zhu, D., et al. 2020. . Angiotensin (1-7) through modulation of the NMDAR–nNOS–NO pathway and serotonergic metabolism exerts an anxiolytic-like effect in rats. Behavioral Brain Research, 390, p.112671. |
| 27127657 | not listed | Jang, S.S., et al. 2016. Seizure-Induced Regulations of Amyloid-β, STEP61, and STEP61 Substrates Involved in Hippocampal Synaptic Plasticity. Neural plasticity, 2016. |
| 26391783 | not listed | Jang, S.S., et al. 2015. Regulation of STEP61 and tyrosine-phosphorylation of NMDA and AMPA receptors during homeostatic synaptic plasticity. Molecular brain, 8. |
| 26277342 | not listed | Mao, L.M., et al. 2015. Dopaminergic and cholinergic regulation of Fyn tyrosine kinase phosphorylation in the rat striatum in vivo. Neuropharmacology, 99, 491-499. |
| 24611998 | 1:1000 | Chen, W., et al. 2014. BDNF released during neuropathic pain potentiates NMDA receptors in primary afferent terminals. European Journal of Neuroscience, 39(9), 1439-1454. |
| 21464302 | 1:1000 | Hinklin, T.R., et al. 2011. Alcohol inhibition of the NMDA receptor function, long-term potentiation, and fear learning requires striatal-enriched protein tyrosine phosphatase. PNAS, Apr 2011; 108: 6650 - 6655. |
| 21215796 | 1:1000 | Castillo, C., et al. 2011. The N-methyl-D-aspartate-evoked cytoplasmic calcium increase in adult rat dorsal root ganglion neuronal somata was potentiated by substance P pretreatment in a protein kinase C-dependent manner. Neuroscience, 177, pp.308-320. |
| 20427654 | 1:1000 | Kurup, P., et al. 2010. Aβ-mediated NMDA receptor endocytosis in Alzheimer's disease involves ubiquitination of the tyrosine phosphatase STEP61. Journal of Neuroscience, 30(17), pp.5948-5957. |
| 19625523 | 1:1000 | Xu, J., et al. 2009. Extrasynaptic NMDA receptors couple preferentially to excitotoxicity via calpain-mediated cleavage of STEP. Journal of Neuroscience, 29(29), pp.9330-9343. |
| 12536146 | 1:1000 | Alvested, R.M., et al. 2003. Tyrosine dephosphorylation and ethanol inhibition of N-methyl-D-aspartate receptor function. Journal of Biological Chemistry, 278(13), pp.11020-11025. |
Product Specific Protocols
- Western Blot Protocol: Download