Anti-NMDA NR2A Subunit (Tyr1325) Antibody

6 Citations

Our Anti-NMDA NR2A Subunit (Tyr1325) rabbit polyclonal phosphospecific primary antibody from PhosphoSolutions is produced in-house. It detects human, mouse, and rat NMDA NR2A Subunit (Tyr1325) and is antigen affinity purified from pooled serum. It is great for use in WB.

Western blot of rat hippocampal lysate showing specific immunolabeling of the ~180 kDa NR2A subunit of the NMDAR phosphorylated at Tyr1325 in the first lane (-). Phosphospecificity is shown in the second lane (+) where immunolabeling is completely eliminated by lysate treatment with lambda phosphatase (400 units/100uL lysate for 30 min).
Western blot of rat hippocampal lysate showing specific immunolabeling of the ~180 kDa NR2A subunit of the NMDAR phosphorylated at Tyr1325 in the first lane (-). Phosphospecificity is shown in the second lane (+) where immunolabeling is completely eliminated by lysate treatment with lambda phosphatase (400 units/100uL lysate for 30 min).
Western blot of rat hippocampal lysate showing specific immunolabeling of the ~180 kDa NR2A subunit of the NMDAR phosphorylated at Tyr1325 in the first lane (-). Phosphospecificity is shown in the second lane (+) where immunolabeling is completely eliminated by lysate treatment with lambda phosphatase (400 units/100uL lysate for 30 min).

Mouse, Rat
WB
Rabbit

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Data Sheet
Trial Size Trial Size Pooled Serum Pooled Serum

SKU: p1514-1325

Volume: 100 µL
1-2 business days
Price:
$430.00

Product Specific References for Applications and Species

Western Blot: Human
PMID Dilution Publication
31135041 1:1000 Dedek, A., et al. 2019. Loss of STEP61 couples disinhibition to N-methyl-d-aspartate receptor potentiation in rodent and human spinal pain processing. Brain, 142(6), pp.1535-1546.
Western Blot: Mouse
PMID Dilution Publication
34433031 not listed Shi, X., et al. 2021. Disrupting phosphorylation of Tyr-1070 at GluN2B selectively produces resilience to depression-like behaviors. Cell Reports, 36(8), p.109612.
34044147 1:1000 Zhang, L., et al. 2021. Tyrosine phosphatase PTP1B impairs presynaptic NMDA receptor-mediated plasticity in a mouse model of Alzheimer's disease. Neurobiology of Disease, 156, p.105402.
Western blot: Rat
PMID Dilution Publication
33333103 1:200 Avchalumov, Y., et al. 2020. Chronic ethanol exposure differentially alters neuronal function in the medial prefrontal cortex and dentate gyrus. Neuropharmacology, Dec. 15, p.108438.
31835746 1:200 Somkuwar, S.S., et al. 2019. Individual Differences in Ethanol Drinking and Seeking Behaviors in Rats Exposed to Chronic Intermittent Ethanol Vapor Exposure is Associated with Altered CaMKII Autophosphorylation in the Nucleus Accumbens Shell. Brain Sciences, 9(12), p.367.
31135041 1:1000 Dedek, A., et al. 2019. Loss of STEP61 couples disinhibition to N-methyl-d-aspartate receptor potentiation in rodent and human spinal pain processing. Brain, 142(6), pp.1535-1546.
29441405 1:200 Takashima, Y., et al. 2018. Neuroadaptations in the dentate gyrus following contextual cued reinstatement of methamphetamine seeking. Brain Structure and Function, pp.1-15.
26220171 1:200 Staples, M.C., et al. 2015. Developmental effects of wheel running on hippocampal glutamate receptor expression in young and mature adult rats. Neuroscience. Jul 25. pii: S0306-4522(15)00672-7.

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