Anti-NMDA NR2B Subunit (Tyr1252) Antibody

Our Anti-NMDA NR2B Subunit (Tyr1252) rabbit polyclonal phosphospecific primary antibody from PhosphoSolutions is produced in-house. It detects mouse and rat NMDA NR2B Subunit (Tyr1252) and is antigen affinity purified from pooled serum. It is great for use in WB, IHC.



SKU: p1516-1252

Volume: 100 µL
1-2 business days
Price:
Sale price$445.00

Product Details

NMDA NR2B Subunit (Tyr1252)
The NMDA receptor (NMDAR) plays an essential role in memory, neuronal development and it has also been implicated in several disorders of the central nervous system including Alzheimer’s, epilepsy and ischemic neuronal cell death (Grosshans et al., 2002; Wenthold et al., 2003; Carroll and Zukin, 2002). The rat NMDAR1 (NR1) was the first subunit of the NMDAR to be cloned. The NR1 protein can form NMDA activated channels when expressed in Xenopus oocytes but the currents in such channels are much smaller than those seen in situ. Channels with more physiological characteristics are produced when the NR1 subunit is combined with one or more of the NMDAR2 (NR2 A-D) subunits (Ishii et al., 1993). Phosphorylation of Tyr-1252 is thought to potentiate NMDA receptor-dependent influx of calcium (Takasu et al., 2002).
Antigen Affinity Purified from Pooled Serum
Polyclonal
IgG
IHC, WB
Rabbit
GRIN2B
180 kDa
Synthetic phospho-peptide corresponding to amino acid residues surrounding Tyr1252 of the NR2B subunit of the rat NMDA receptor, conjugated to keyhole limpet hemocyanin (KLH).
Mouse, Rat
Bovine, Canine, Chicken, Human, Non-Human Primate, Zebrafish
AB_2492180
Storage at -20°C is recommended, as aliquots may be taken without freeze/thawing due to presence of 50% glycerol. Stable for at least 1 year at -20°C.
Liquid
Prepared from pooled rabbit serum by affinity purification via sequential chromatography on phospho and non-phosphopeptide affinity columns.
10 mM HEPES (pH 7.5), 150 mM NaCl, 100 µg per ml BSA and 50% glycerol.
WB: 1:1000
WB Brain: 1:1000
ICC: 1:100-1:400
Unconjugated
Specific for endogenous levels of the ~180 kDa NMDAR NR2B-subunit protein phosphorylated at Tyr1252 . Immunolabeling is completely eliminated by treatment with λ-phosphatase. May also show slight reactivity with Tyr1246 of NR2A.
Phosphorylated
Tyr1252
Western blots performed on each lot.
For research use only. Not intended for therapeutic or diagnostic use. Use of all products is subject to our terms and conditions, which can be viewed on our website.
After date of receipt, stable for at least 1 year at -20°C.
Blue Ice
EPND antibody, FESD antibody, GluN2A antibody, Glutamate [NMDA] receptor subunit epsilon-1 antibody, Glutamate receptor antibody, Glutamate receptor ionotropic N methyl D aspartate 2A antibody, GRIN 2A antibody, GRIN2A antibody, hNR2A antibody, LKS antibody, N methyl D aspartate receptor channel subunit epsilon 1 antibody, N Methyl D Aspartate Receptor Subtype 2A antibody, N methyl D aspartate receptor subunit 2A antibody, N-methyl D-aspartate receptor subtype 2A antibody, NMDA receptor subtype 2A antibody, NMDAR 2A antibody, NMDAR2A antibody, NMDE1_HUMAN antibody, NR2A antibody, OTTHUMP00000160135 antibody, OTTHUMP00000174531 antibody
UniProt (Human): Q13224
UniProt (Immunogen Species): Q00960
24410

Product Specific References for Applications and Species

Immunocytochemistry: Rat
PMID Dilution Publication
32651756 1:100 Akkuratov, E.E., et al. 2020. Ouabain Modulates the Functional Interaction Between Na, K-ATPase and NMDA Receptor. Molecular Neurobiology, pp.1-13.
Western Blot: Mouse
PMID Dilution Publication
32946951 1:1000 Diab, A., et al. 2020. NCK1 regulates amygdala activity to control context-dependent stress responses and anxiety in male mice. Neuroscience, 448, pp 107-125.
25158771 not listed Knox, R., et al. 2014. NR2B phosphorylation at tyrosine 1472 contributes to brain injury in a rodent model of neonatal hypoxia-ischemia. Stroke, 45(10), pp.3040-3047.
21464302 1:1000 HIcklin, T.R., et al. 2011. Alcohol inhibition of the NMDA receptor function, long-term potentiation, and fear learning requires striatal-enriched protein tyrosine phosphatase. PNAS, Apr 2011; 108: 6650 - 6655.
Western Blot: Rat
PMID Dilution Publication
21464302 1:1000 HIcklin, T.R., et al. 2011. Alcohol inhibition of the NMDA receptor function, long-term potentiation, and fear learning requires striatal-enriched protein tyrosine phosphatase. PNAS, Apr 2011; 108: 6650 - 6655.

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