Our Anti-Alpha Internexin/ NF-66 primary antibody from PhosphoSolutions is chicken polyclonal. It detects human, mouse, and rat Alpha Internexin/ NF-66 and is total IgY fraction. It is great for use in WB, IHC, ICC.
Immunofluorescence of a section of rat cerebellum showing labeling of axons of granule cell and dendrites of Purkinje with alpha-internexin (cat. 101-AIN, 1:5000, red), labeling of the myelin sheaths around the axons with MBP (cat. 1120-MBP, 1:5000, green), and additional nuclear staining with DAPI (blue).
Alpha-internexin is a Class IV intermediate filament originally discovered as it co-purifies with other neurofilament subunits (1). Alpha-internexin is related to but distinct from the better known neurofilament triplet proteins, NF-L, NF-M and NF-H, having similar protein sequence motifs and a similar intron organization. It is expressed only in neurons and in large amounts early in neuronal development, but is down-regulated in many neurons as development proceeds. Many classes of mature neurons contain alpha-internexin in addition to NF-L, NF-M and NF-H. In some mature neurons alphainternexin is the only neurofilament subunit expressed. Antibodies to alpha-internexin are therefore unique probes to study and classify neuronal types and follow their processes in sections and in tissue culture. In addition, recent studies show a marked up-regulation of alpha-internexin during neuronal regeneration (2). The use of antibodies to this protein in the study of brain tumors has not been examined to date, but is likely to be of interest. Recently Cairns et al. used this antibody to show that alphainternexin is an abundant component of the inclusions of neurofilament inclusion body disease (NFID), a serious human neurodegenerative disorder (3,4). The antibody was also used to confirm the presence of circulating auto-antibodies to alpha-internexin in the sera of some patients with endocrine autoimmunity, as well as in some normal individuals (5).
Total IgY fraction
Polyclonal
IgY
ICC, IHC, WB
Chicken
INA
66 kDa
Recombinant rat alpha-internexin expressed in and purified from E. coli
Bovine, Human, Mouse, Rat
AB_2492033
Recommended that the undiluted antibody be aliquoted into smaller working volumes (10-30 uL/vial depending on usage) upon arrival and stored long term at -20° C or -80° C, while keeping a working aliquot stored at 4° C for short term. Avoid freeze/thaw cycles. Stable for at least 1 year.
Specific for endogenous levels of the ~66 kDa alpha Internexin protein. Minor bands at ~150 kDa are probably covalent dimers and bands at ~50 kDa represent alpha-internexin breakdown products.
Western blots performed on each lot.
For research use only. Not intended for therapeutic or diagnostic use. Use of all products is subject to our terms and conditions, which can be viewed on our website.
After date of receipt, stable for at least 1 year at -20°C.
Pachter, J.S. and Liem, R.K., 1985. alpha-Internexin, a 66-kD intermediate filament-binding protein from mammalian central nervous tissues. The Journal of Cell Biology, 101(4), pp.1316-1322. PMID: 2413040
McGraw, T.S., Mickle, J.P., Shaw, G. and Streit, W.J., 2002. Axonally transported peripheral signals regulate α-internexin expression in regenerating motoneurons. Journal of Neuroscience, 22(12), pp.4955-4963. PMID: 12077192
Cairns, N.J., Uryu, K., Bigio, E.H., Mackenzie, I.R., Gearing, M., Duyckaerts, C., Yokoo, H., Nakazato, Y., Jaros, E., Perry, R.H. and Arnold, S.E., 2004. α-Internexin aggregates are abundant in neuronal intermediate filament inclusion disease (NIFID) but rare in other neurodegenerative diseases. Acta Neuropathologica, 108(3), pp.213-223. PMID: 15170578
Cairns, N.J., Zhukareva, V., Uryu, K., Zhang, B., Bigio, E., Mackenzie, I.R., Gearing, M., Duyckaerts, C., Yokoo, H., Nakazato, Y. and Jaros, E., 2004. α-Internexin is present in the pathological inclusions of neuronal intermediate filament inclusion disease. The American Journal of Pathology, 164(6), pp.2153-2161. PMID: 15161649
Rajasalu, T., Teesalu, K., Janmey, P.A. and Uibo, R., 2004. Demonstration of natural autoantibodies against the neurofilament protein α-internexin in sera of patients with endocrine autoimmunity and healthy individuals. Immunology Letters, 94(1-2), pp.153-160. PMID: 15234547
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