Polyclonal Antibodies

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Western blot of human T47D cells showing specific immunolabeling of the ~34 kDa cdc2 phosphorylated at Tyr15 (Control, Lane 1). Treatment with EGF (30 ng per ml for 30 min) caused dephosphorylation of the Tyr15 on cdc2 (Lane 2). Phosphospecificity is shown in lanes 3 and 4, where the immunolabeling is completely eliminated by blot treatment with lambda phosphatase, λ-Ptase (1200 units for 30 min).
PhosphoSolutions Anti-cdc2 (Tyr15) Antibody
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Western blot of OCIAML2 lysate showing specific immunolabeling of the ~51 kDa MEF2C phosphorylated at Ser222 in the first lane (-). Phosphospecificity is shown in the second lane (+) where the immunolabeling is completely eliminated by blot treatment with lambda phosphatase ((λ-Ptase, 1200 units for 30 minutes).
PhosphoSolutions Anti-MEF2C (Ser222) Antibody
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Western blot of rat cortical lysate showing specific immunolabeling of the ~45 kDa GABAA γ2 protein phosphorylated at Ser327 in the first lane (-). Phosphospecificity is shown in the second lane (+) where immunolabeling is blocked by preadsorption of the phosphopeptide used as the antigen, but not by the corresponding non-phosphopeptide (not shown).
PhosphoSolutions Anti-GABAA Receptor γ2 (Ser327) Antibody
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Western blot of rat kidney lysate showing specific immunolabeling of the ~29 kDa and 37 kDa glycosylated form of the AQP2 protein phosphorylated at Ser264 in the vasopressin (AVP) treated lane (+), but not in the control lane (-).
PhosphoSolutions Anti-Aquaporin 2 (Ser264) Antibody
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Western blot of rat hippocampal lysate showing specific immunolabeling of the ~43 kDa connexin43 phosphorylated at Ser368 in the first lane (-). Phosphospecificity is shown in the second lane (+) where immunolabeling is completely eliminated by lysate treatment with lambda phosphatase (λ-Ptase, 800 units/1mg protein for 30 min).
PhosphoSolutions Anti-Connexin 43 (Ser368) Antibody
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Western blot of a rat hippocampal lysate showing the specific immunolabeling of the ~100 kDa GluR2 protein.
PhosphoSolutions Anti-GluR2 Antibody
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Western blot of rat cortical lysate showing specific immunolabeling of the ~43 kDa GAP43 protein.Immunolabeling of mixed neuron and glial cultures with anti-GAP43 (cat. 875-GAP43, 1:2000, green), alpha II spectrin (cat. 99-A2SR, 1:1000, red) and nuclear staining with DAPI (blue). The anti-GAP43 labels protein expressed in the axonal membrane and synapses of neuronal cells. The Anti-αII-Spectrin labels the submembraneous cytoskeleton of axons and dendrites.
PhosphoSolutions Anti-GAP43 Antibody
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Western blot of 7 µg of rat cerebellar lysate showing specific immunolabeling of the ~55 kDa β2-subunit of the GABAA-R.
PhosphoSolutions Anti-GABAA Receptor ß2 Antibody
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Western blot of mouse whole brain lysate showing specific immunolabeling of the ~46 kDa γ2-subunit of GABAA Receptor.Immunostaining of mouse retina showing specific labeling of the GABAA-R γ2 subunit (cat. 832A-GG2C, green, 1:300) and calbindin (red). The blue stain is DAPI staining nuclear DNA. Photo courtesy of Dr. Arlene Hirano, UCLA.
PhosphoSolutions Anti-GABAA Receptor γ2 Antibody
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Western blot of mouse brain lysates from wild type (WT) and α3-knockout (K/O) animals showing specific immunolabeling of the ~51 kDa α3-subunit of the GABAA-R. The labeling was absent from a lysate prepared from α3-knockout animals. Immunostaining of rat amygdala showing labeling of GABAA α3 subunit (DAB, 1:100).
PhosphoSolutions Anti-GABAA Receptor α3 Antibody
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Western blot of mouse forebrain lysates from Wild Type (WT) and α1-knockout (K/O) animals showing specific immunolabeling of the ~51 kDa α1-subunit of the GABAA-R. The labeling was absent from a lysate prepared from α1-knockout animals.
PhosphoSolutions Anti-GABAA Receptor α1, N-Terminus Antibody
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Western blot of human striatal lysate showing specific immunolabeling of the ~88 kDa DAT protein.
PhosphoSolutions Anti-Dopamine Transporter, Extracellular Loop 2 Antibody
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Immunochemical staining of cultured CD1 mouse striatal cells showing specific labeling of DARPP-32(cat. 374-DARPP, green, 1:250) and Tuj1(red). Cells and photo courtesy of QBM CellScience.Western blot of rat hippocampal lysate showing specific immunolabeling of the ~32 kDa DARPP protein.
PhosphoSolutions Anti-DARPP-32 Antibody
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Immunochemical staining of saline treated mouse cortex cryosections showing specific labeling of CNP(cat. 325-CNP, green, 1:500). The blue is staining DNA. The bisected longitudinal mouse brain was fixed for 18h in 4% paraformaldehyde, washed, and cryoprotected in 30% sucrose overnight. The cryosections were antigen retrieved in citrate buffer, blocked in 20% normal goat serum for 1 hr. Photo courtesy of Robert Wine.Western blot of rat brain lysate showing the specific immunolabeling of the ~46 kDa CNP protein.
PhosphoSolutions Anti-CNP (2,3-cyclic nucleotide-3-phosphodiesterase) Antibody
$385
Immunostaining of 14 DIV rat cortical neurons showing NR2B (green, 1:400) and PSD95(red). Photo courtesy of Gang Liu. Western blot of 10 µg of rat hippocampal lysate showing specific immunolabeling of the ~180 kDa NR2B subunit of the NMDA receptor.
PhosphoSolutions Anti-NMDA NR2B Subunit, N-terminus Antibody
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Western blot of 10 µg of rat hippocampal lysate showing specific immunolabeling of the ~180 kDa NR2A subunit. Immunostaining of 21 DIV nucleofected mouse striatal neuron (green) co-cultured with cortical neurons showing nice punctate labeling of the N-terminal NR2A subunit (red, 1:500) in both the medium spiny neurons and the large pyramidal cell in the upper right. Photo courtesy of Dr. A.J. Milnerwood, Dr. Lynn Raymond Lab, University of British Columbia.
PhosphoSolutions Anti-NMDA NR2A Subunit, N-terminus Antibody
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Western blot of 10 µg of rat hippocampal lysate showing specific immunolabeling of the ~180 kDa NR2B subunit of the NMDA receptor.
PhosphoSolutions Anti-NMDA NR2B Subunit Antibody
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Immunofluorescent staining of HeLa cells using 2 µg/mL Aves Labs chicken anti-Calnexin (CANX) (Cat. No. CANX-0100) antibody (green). Actin filaments were stained using Phalloidin (red). DAPI nuclear stain (blue) shows cell nuclei. The cells were mounted with Antibodies Incorporated Fluoroshield with DAPI mounting medium (Cat. No. AR-6501). Anti-Calnexin specifically stains the Endoplasmic Reticulum in HeLa cells. ​Western blotting of various cell lysates with Aves Labs chicken anti-Calnexin (CANX) antibody (0.05 µg/ml) and detected with anti-chicken HRP. Aves Labs Chicken anti-Calnexin recognizes endogenous Calnexin in all the cell lysates at ~90 kDa.
Aves Labs Anti-Calnexin Antibody
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Metabotropic glutamate receptor type 2 (mGluR2, 1:1000 dilution, green) immunoreactivity and neurofilament-M (NF-M, rabbit antibody, 1:500 dilution, red) immunoreactivity in a tissue section through an e18 mouse cochlear ganglion.Metabotropic glutamate receptor type 2 (mGluR2, 1:1000 dilution, green) immunoreactivity and neurofilament-M (NF-M rabbit antibody, 1:500 dilution, red) immunoreactivity in a tissue section through the CA3 hippocampal formation of an e18 mouse embryo.
Aves Labs Anti-Metabotropic Glutamate Receptor Type 2 / Type 3 (mGluR2/mGluR3) Antibody
$470
Metabotropic glutamate receptor type 1 (1:1000 dilution, green) immunoreactivity and neurofilament-M (NF-M, rabbit antibody, 1:500 dilution) immunoreactivity in a tissue section through an e18 mouse cochlear ganglion.Metabotropic glutamate receptor type 1 (1:1000 dilution, green) immunoreactivity and neurofilament-M (NF-M, rabbit antibody, 1:500 dilution) immunoreactivity in a tissue section through an e18 mouse cochlear ganglion.
Aves Labs Anti-Metabotropic Glutamate Receptor Type 1 (mGluR1) Antibody
$470
Anti-Polyhistidine Anti-6XHIS (for Western) Antibody
Aves Labs Polyhistidine Anti-6XHIS Antibody (for Westerns)
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HeLa cells were subjected to immunofluorescent staining using chicken anti-TOMM20 antibody (visualized in green) and Antibodies Inc mouse anti-Mortalin antibody (75-127) (visualized in red). DAPI nuclear stain (blue) shows cell nuclei. The cells were mounted with ICT's Fluoroshield with DAPI mounting medium (AR-6501). The staining revealed a complete overlap between the signal from the chicken anti-TOMM20 antibody and the mouse anti-Mortalin antibody specifically in the cell mitochondria.Western blotting of various cell lysates with chicken anti-TOMM20 antibody (0.2 µg/ml)(1:1000) and detected with anti-chicken HRP. Chicken anti-TOMM20 recognizes endogenous TOMM20 in all the cell lysates at ~16 kDa.
Aves Labs Anti-TOMM20 Antibody
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Aves Labs Goat Anti-Chicken IgY (IgG) (H+L) Secondary Antibody, CF®647
$164
Western blot of human SYF cSrc-transformed cells. Blots were were probed with anti-WAVE1 (N-terminal region) at a dilution of 1:1000 (lane 1), 1:2000 (lane 2) or 1:4000 (lane 3). In addition, the antibody was used in the absence (lane 4) or presence of blocking peptides, WAVE1 (N-terminal region) peptide (lane 5) or WAVE2 (Central region) peptide (lane 6).Immunocytochemical labeling of phosphorylated WAVE in pervanadate-treated mouse C2C12. The cells were labeled with rabbit polyclonal WAVE1 (N-terminal region) and WAVE (Tyr-125) antibodies, then the antibodies were detected using appropriate secondary antibodies conjugated to Cy3.
PhosphoSolutions Anti-WAVE1 (N-terminal region) Antibody
$340

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