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Western Blot of 50 ug of mouse brain lysate showing specific immunolabeling of SHPRH.
Western blot analysis of SMAD7 in human HepG2 cells. The blot was probed with rabbit polyclonal SMAD7 antibody (SP4901) at 1:250.
Western blot of NIH 3T3 cell lysate showing specific immunolabeling of the ~35 kDa SOX2 protein.Immunofluoresence of FLAG-tagged SOX2 transfected COS cells showing SOX2 staining in red (cat. 1907-SOX2, 1:20) and FLAG staining in green, causing positive transfected cells to appear yellow. DNA is stained blue with DAPI.
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Western blot image of mouse F9 stem cells treated with with calyculin A (100 nM, 30 min.) (lanes 1-4) then Sox2 was dephosphorylated with lambda phosphatase (lanes 2 & 4). The blot was probed with mouse monoclonal Sox2 (lanes 1 & 2) and rabbit polyclonal anti-Sox2 (Thr-118) phospho-specific antibody (lanes 3 & 4).Immunocytochemical labeling of Sox2 in aldehyde fixed and NP-40 permeabilized human NCI-H446 lung carcinoma cells. The cells were labeled with mouse monoclonal anti-Sox2 (SM5511). The antibody was detected using goat anti-mouse DyLight® 594.
Western blot analysis of human A431 cells untreated (lanes 1, 3, 5, 7 & 9) or treated with EGF (100 nM) for 60 min (lanes 2, 4, 6, 8 & 10). The blots were probed with anti-Stat1 (lanes 1 & 2), anti-Stat1 (Tyr-701) (lanes 3 & 4), anti-Stat3 (lanes 5 & 6), anti-Stat5 (lanes 7 & 8), and anti-Stat5 (Tyr-694) (lanes 9 & 10).Immunocytochemical labeling of Stat1 in human A431 cells. The cells were labeled with mouse monoclonal Stat1 (SM2491) antibody (Right), then the antibody was detected using appropriate secondary antibody conjugated to DyLight® 594. Corresponding phase image is shown to the left.
Western blot analysis of human A431 cells untreated (lanes 1, 3, 5, 7, & 9) or treated with EGF (100 nM) for 60 min (lanes 2, 4, 6, 8, & 10). The blots were probed with anti-Stat1 (lanes 1 & 2), anti-Stat1 (Tyr-701) (lanes 3 & 4), anti-Stat3 (lanes 5 & 6), anti-Stat5 (lanes 7 & 8), and anti-Stat5 (Tyr-694) (lanes 9 & 10).
Western blot analysis of human A431 cells untreated (lanes 1, 3, 5, 7, & 9) or treated with EGF (100 ng/ml for 60 min (lanes 2, 4, 6, 8, & 10). The blots were probed with anti-Stat1 (lanes 1 & 2), anti-Stat1 (Tyr-701) (lanes 3 & 4), anti-Stat3 (lanes 5 & 6), anti-Stat5 (lanes 7 & 8), and anti-Stat5 (Tyr-694) (lanes 9 & 10).Immunocytochemical labeling of Stat5 in control and pervanadate-treated A431 cells. The cells were labeled with mouse monoclonal Stat5 (SM2511) or Stat5 (Tyr-694) (SM1481) antibodies, then the antibodies were detected using appropriate secondary antibody conjugated to DyLight® 594.

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