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Western blot analysis of human cell lysates: A549 (lane 1), A431 (lane 2), LNCaP (lane 3), MDA-MB-231 (lane 4) and MeWo (lane 5). The blot was probed with mouse monoclonal anti-CD99 (CM0261) at 1:1000.Immunocytochemical labeling of CD99 in paraformaldehyde fixed human MeWo cells. The cells were labeled with mouse monoclonal anti-CD99 (clone M026). The antibody was detected using goat anti-mouse DyLight® 594.
PhosphoSolutions Anti-CD99 (Extracellular region) Antibody
$370
Immunocytochemical labeling of CD99 in paraformaldehyde fixed human MeWo cells. The cells were labeled with mouse monoclonal anti-CD99 (clone M030). The antibody was detected using goat anti-mouse DyLight® 594.
PhosphoSolutions Anti-CD99 (Extracellular region) Antibody
$340
Western blot of human T47D cells showing specific immunolabeling of the ~34 kDa cdc2 phosphorylated at Tyr15 (Control, Lane 1). Treatment with EGF (30 ng per ml for 30 min) caused dephosphorylation of the Tyr15 on cdc2 (Lane 2). Phosphospecificity is shown in lanes 3 and 4, where the immunolabeling is completely eliminated by blot treatment with lambda phosphatase, λ-Ptase (1200 units for 30 min).
PhosphoSolutions Anti-cdc2 (Tyr15) Antibody
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Western blot analysis of human jurkat cells (lanes 1 & 2) and mouse brain (lanes 3 & 4). The blots were probed with anti-Cdc42 antibody at 1:125 (lanes 1 & 3) or 1:500 (lanes 2 & 4).Immunocytochemical labeling in rat PC12 cells grown for 4 days on poly-D-lysine-coated plates in the presence (200 ng/ml) or absence (Control) of Nerve Growth Factor (NGF). Anti-Cdc42 (CM1521) was used at 1:50 dilution followed by labeling with donkey anti-mouse:Cy2.
PhosphoSolutions Anti-Cdc42 Antibody
$285
Immunocytochemical labeling in paraformaldehyde fixed and NP-40 permeabilized rat A7r5 cells. The cells were labeled with mouse monoclonal Anti-Cdc42 (CM4301) in the absence (Left) or presence of full length Cdc42 recombinant protein (Right), then the antibody was detected using Goat anti-Mouse secondary antibody conjugated to DyLight® 594.
PhosphoSolutions Anti-Cdc42 Antibody
$285
Western blot analysis of human SYF fibroblasts before (lanes 1 & 3) and after (lanes 2 & 4) treatment with alkaline phosphatase. The blots were probed with anti-Cdk1 (N-terminal region) antibody (lanes 1 & 2) or anti-Cdk1 (Tyr-15) phospho-specific antibody (lanes 3 & 4).
PhosphoSolutions Anti-Cdk1 (N-terminal region) Antibody
$285
Western blot analysis of human SYF fibroblasts before (lanes 1 & 3) and after (lanes 2 & 4) treatment with alkaline phosphatase. The blots were probed with anti-Cdk1 (N-terminal region) antibody (lanes 1 & 2) or anti-Cdk1 (Tyr-15) phospho-specific antibody (lanes 3 & 4).
PhosphoSolutions Anti-Cdk1 (Tyr-15) [conserved site], Phosphospecific Antibody
$340
Western blot of rat striatal lysate showing specific immunolabeling of the ~28 kDa Cdk5 protein.
PhosphoSolutions Anti-CDK5 Antibody
From $160
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Western blot analysis of human HeLa cells. The blot was probed with anti-Cdk5 (CM2361) antibody at 1:250 (lane 1), 1:1000 (lane 2), and 1:4000.(lane 3).
PhosphoSolutions Anti-Cdk5 Antibody
$285
Western blot of rat hippocampal lysate showing specific immunolabeling of the ~70 kDa ChAT. Immunostaining of rat brainstem showing specific labeling using our anti-ChAT antibody. Image courtesy of Dr. Robert Sloviter, University of Arizona.
PhosphoSolutions Anti-ChAT (Choline Acetyltransferase) Antibody
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Western blot of rat hippocampal lysate showing specific immunolabeling of the ~68/70 kDa ChAT.
PhosphoSolutions Anti-ChAT (Choline Acetyltransferase) Antibody
From $150
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Western blot of HeLa cell lysate showing specific immunolabeling of the ~66 kDa Che-1 protein phosphorylated at Ser477 in the first lane (-). Phosphospecificity is shown in the second lane (+) where immunolabeling is blocked by preadsorption of the phosphopeptide used as the antigen, but not by the corresponding non-phosphopeptide (not shown).
PhosphoSolutions Anti-Che-1 (AATF) (Ser477) Antibody
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Western Blot of 30ug HepG2 cell lysate showing specific immunolabeling of CIDE3.
PhosphoSolutions Anti-CIDE3 Antibody
$445
Western blot of mouse mitochondria from whole brain lysate showing specific immunolabeling of the ~46 kDa CK1Mt protein phosphorylated at Tyr153 in the first lane (-). Phosphospecificity is shown in the second lane (+) where the immunolabeling is greatly decreased by blot treatment with lambda phosphatase (λ-Ptase, 1200 units for 30 minutes).
PhosphoSolutions Anti-CK1Mt (Tyr153) Antibody
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Western Blot of 100 ug of formic acid extracts of Alzheimer diseased brain showing specific immunolabeling of Collagen XXV.
PhosphoSolutions Anti-CLAC-P (NC2-1 region) Antibody
$445
PhosphoSolutions Anti-CLAC-P (NC2-2 region) Antibody
$445
Immunohistochemistry of the neocortex of Alzheimer's diseased brain sections (floating, fixed with 10% formalin for 24 hrs) showing specific staining of Collagen XXV. Western Blot of Lane 1: full-length CLAC-P (lysate), Lane 2: secreted form of CLAC-P (media), Lane 3: COLXIII (lysate), Lane 4 degrees COLXIII (media), Lane 5: COLXXIII (lysate), Lane 6: COLXXIII (media) showing specific immunolabeling of Collagen XXV alpha 1.
PhosphoSolutions Anti-CLAC-P (NC3 region) Antibody
$445
Western Blot of lung lysate showing specific immunolabeling of CLAN. This antibody detects CLAN-A and CLAN-B isoforms.Western Blot showing specific immunolabeling of cN-II and Ipaf (CARD12) protein expression in different human cell lines. Image from the following publication (https://dx.plos.org/10.1371/journal.pone.0121525) licensed under a CC-BY license.
PhosphoSolutions Anti-CLAN (CARD12) Antibody
$445
Western blot of CLASP1 in mouse brain (lane 1) and rat PC12 cells (lane 2). The blots were probed with rat monoclonal CM5011 anti-CLASP1 (C-terminus) at a dilution of 1:500. Then detected using donkey anti-Rat IgG:HRP (RS3101).
PhosphoSolutions Anti-CLASP1 (C-terminus) Antibody
$285
Western blot of CLASP2 in mouse brain (lanes 1 & 3) and rat PC12 cells (lanes 2 & 4). The blots were probed with rat monoclonals CM5051 anti-CLASP2 (clone KT68) (lanes 1 & 2) and CM5071 anti-CLASP2 (clone KT69) (lanes 3 & 4). The antibodies were used at a dilution of 1:500.Immunocytochemical labeling of CLASP2 in paraformaldehyde-fixed and NP40-permeabilized A431 cells. The cells were dual labeled with mouse monoclonal anti-α-Tubulin (TM4111) (left) and rat monoclonal anti-CLASP2 (CM5051) (middle). The antibodies were detected using either goat anti-mouse:DyLight® 488 (MS3011) or goat anti-Rat:DyLight® 594 (RS3111).
PhosphoSolutions Anti-CLASP2 (N-terminus) Antibody
$285
Western blot of rat hippocampal lysate showing specific immunolabeling of the ~35 kDa clavesin protein.
PhosphoSolutions Anti-Clavesin Antibody
From $160
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Immunochemical staining of saline treated mouse cortex cryosections showing specific labeling of CNP(cat. 325-CNP, green, 1:500). The blue is staining DNA. The bisected longitudinal mouse brain was fixed for 18h in 4% paraformaldehyde, washed, and cryoprotected in 30% sucrose overnight. The cryosections were antigen retrieved in citrate buffer, blocked in 20% normal goat serum for 1 hr. Photo courtesy of Robert Wine.Western blot of rat brain lysate showing the specific immunolabeling of the ~46 kDa CNP protein.
PhosphoSolutions Anti-CNP (2,3-cyclic nucleotide-3-phosphodiesterase) Antibody
$385
Immunostaining of mouse striatum showing specific labeling of CNP in myelinating oligodendrocytes (Cat. # 326-CNP, green, 1:3000) and neurofilament L (red, 1:5000). The blue is staining DNA. Western blot of rat hippocampal lysate showing the specific immunolabeling of the ~46 kDa CNP protein.
PhosphoSolutions Anti-CNP (2,3-cyclic nucleotide-3-phosphodiesterase) Antibody
From $160
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Western Blot of 10 ug of normal human brain lysate showing specific immunolabeling of CNPase.
PhosphoSolutions Anti-CNPase Antibody
$445

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