PhosphoSolutions

Western blot of neonatal rat brain lysate showing specific immunolabeling of the ~62 kDa COBRA1 protein.

PhosphoSolutions Anti-COBRA1 (NELF-B) Antibody

From $160

Western blot of cofilin in jurkat cells. The blots were untreated (lanes 1 & 4) or treated (lanes 2, 3, 5 & 6) with lambda phosphatase. In lanes 3 & 6, the phosphatase was inhibited with phospho-Cofilin 1 (Ser-3) peptide. The blots were probed with anti-Cofilin 1 (Ser-3) phospho-specific (lanes 1-3) or anti-Cofilin 1 (N-terminus) (lanes 4-6).

Immunocytochemical labeling in chick dorsal root ganglion neurons using anti-Cofilin (N-terminus; CP1131), anti-Cofilin (Ser-3; CP1151), anti-βIII-Tubulin (C-terminus; TP1691) and anti-β-Tubulin (TM1541) antibodies. (Images provided by Dr. Diane Snow, Department of Anatomy & Neurobiology, University of Kentucky).

PhosphoSolutions Anti-Cofilin 1 (N-terminus) Antibody

$340

PhosphoSolutions Anti-Cofilin 1 (Ser-3), Phosphospecific Antibody

$340

Western blot of rat lung lysate showing specific immunolabeling of the ~180 kDa collagen 1.

Immunostaining of fibrotic mouse lung tissue showing specific staining of collagen I molecules (cat. 621-COLP, 1:100, red) that are still associated with the cells in which they were synthesized. The blue is staining DNA.

PhosphoSolutions Anti-Collagen 1, alpha 1 propeptide Antibody

From $160

Immunostaining of formaldehyde-fixed fibrotic mouse lung tissue. The antibody recognizes mature collagen I (cat. 322-COLT, 1:100, red) that has formed fibrils in the extracellular matrix. The blue is staining nuclear DNA.

Immunofluorescence of a transversal section from mouse spinal cord labeling fibers of collagen (cat. : 322-COLT, red, 1:200) from the tissue scaffold. The blue is DAPI counterstaining nuclear DNA. Image kindly provided by Dr. Rodolfo Gatto and Dr. Gerardo Morfini, Department of Anatomy and Cell biology, University of Illinois at Chicago.

PhosphoSolutions Anti-Collagen 1, alpha 1 telopeptide Antibody

From $160

PhosphoSolutions Anti-COM1/p8 (N-terminus) Antibody

$445

Western blot of connexin35/36 fusion proteins showing specific immunolabeling of the ~35 kDa connexin-35/36 phosphorylated at Ser276. The first lane (1) is PKA phosphorylated protein purified from the C-Terminal domain. The second lane (2) shows phosphospecificity indicated by no labeling in protein without PKA stimulation. The third lane (3) is protein purified from the intracellular loop, demonstrating that there is no cross-reactivity with the other major PKA phosphorylation site at Ser110.

PhosphoSolutions Anti-Connexin 35/36 (Ser276) Antibody

From $160

Western blot of rat hippocampal lysate showing specific immunolabeling of the ~43 kDa connexin43 phosphorylated at Ser368 in the first lane (-). Phosphospecificity is shown in the second lane (+) where immunolabeling is completely eliminated by lysate treatment with lambda phosphatase (λ-Ptase, 800 units/1mg protein for 30 min).

PhosphoSolutions Anti-Connexin 43 (Ser368) Antibody

From $160

Western blot of rat cerebellar lysate showing specific immunolabeling of the ~43 kDa connexin43 protein.

Immunolabeling on astroglial in the CA1 and DG mouse hippocampus, and anterior cingulate cortex (ACC) specifically labeling Connexin43 (Cat no 327-CON, green, 1:200). Image from publication. CC-BY-4.0 PMID: 37239279

PhosphoSolutions Anti-Connexin 43 Antibody

From $160

Western blot analysis of connexin-43 expression in adult mouse brain (lanes 1-3). The blots were probed with mouse monoclonal anti-connexin-43 at 1:250 (lane 1), 1:500 (lane 2), and 1:1000 (lane 3).

PhosphoSolutions Anti-Connexin-43 (C-terminal region) Antibody

$285

Western blot analysis of human A431 cells treated with Calyculin A (100 nM) for 30 min (lanes 1 & 3) before treatment with lambda phosphatase (lanes 2 & 4). The blots were probed with anti-Coronin-1B (C-terminal region) (lanes 1 & 2) and anti-Coronin-1B (Ser-2) (lanes 3 & 4).

Immunocytochemical labeling of coronin-1B in rabbit spleen fibroblasts treated with Calyculin A. The cells were labeled with rabbit polyclonal Coronin-1B (C-terminus) and Coronin-1B (Ser-2) antibodies, then detected using appropriate secondary antibodies conjugated to Cy3. The antibodies were used in the absence (left) or presence (right) of their respective blocking peptide (CX2585 or CX2625).

PhosphoSolutions Anti-Coronin-1B (C-terminus) Antibody

$285

PhosphoSolutions Anti-Coronin-1B (Ser-2), Phosphospecific Antibody

$340

Western blot of rat mitochondrial lysate showing specific immunolabeling of the ~17 kDa COXIV phosphorylated at Ser58 in the first lane (-). Phosphospecificity is shown in the second lane (+) where immunolabeling is blocked by preadsorption of the phosphopeptide used as the antigen, but not by the corresponding non-phosphopeptide (not shown).

PhosphoSolutions Anti-COXIV (Ser58) Antibody

From $160

Western blot analysis of human A431 cells treated with calyculin A (100 nM) for 30 min. (lanes 1, 3 & 5) then the blots were treated with lambda phosphatase (lanes 2, 4 & 6). The blots were probed with anti-CREB (N-terminal region) (lanes 1 & 2), anti-CREB (Ser-133) (lanes 3 & 4), and anti-CREB (Ser-271) (lanes 5 & 6).

Immunocytochemical labeling of CREB in A431 that were fixed in paraformaldehyde and permeabilized using NP-40. The cells were labeled with rabbit polyclonal CREB (N-terminal region). The antibody was detected using goat anti-rabbit DyLight® 594.

PhosphoSolutions Anti-CREB (N-terminal region) Antibody

$285

Immunocytochemical labeling of phosphorylated CREB in calyculin A-treated A431 cells. The cells were fixed in paraformaldehyde and permeabilized using NP-40 before labeling with mouse monoclonal CREB (Ser-133). The antibody was detected using goat anti-mouse DyLight® 594.

PhosphoSolutions Anti-CREB (Ser-133), Phosphospecific Antibody

$285

Western blot analysis of human A431 cells treated with calyculin A (100 nM) for 30 min. (lanes 1, 3, & 5) then blots were treated with lambda phosphatase (lanes 2, 4, & 6). The blots were probed with anti-CREB (N-terminal region) (lanes 1 & 2), anti-CREB (Ser-133) (lanes 3 & 4), and anti-CREB (Ser-271) (lanes 5 & 6).

Immunocytochemical labeling of phosphorylated CREB in control and calyculin A-treated A431 cells. The cells were fixed in paraformaldehyde and permeabilized using NP-40 before labeling with rabbit polyclonal CREB (Ser-271). The antibody was detected using goat anti-rabbit DyLight® 594.

PhosphoSolutions Anti-CREB (Ser-271), Phosphospecific Antibody

$340

Immunolabeling of a section of mouse piriform cortex labeled with Anti-Phospho-Ser133 CREB (cat. p1010-133, red, 1:1000). Cell nuclei are visualized with DAPI DNA stain (blue).

Western blot of rat hippocampal lysate stimulated with forskolin showing specific immunolabeling of the ~45 kDa CREB phosphorylated at Ser133 in the first lane (-). Phosphospecificity is shown in the second lane (+) where immunolabeling is completely eliminated by lysate treatment with lambda phosphatase (λ-Ptase, 800 units/1mg protein for 30 min).

PhosphoSolutions Anti-CREB (Ser133) Antibody

From $160

PhosphoSolutions Anti-CRG-L2 Antibody

$445

Immunofluorescence of HEK293 cells transfected with a fusion construct of GFP with the C-terminal region of S. aureas Cas9 protein (green) that is labeled with Anti-CRISPR Cas9 (cat. 304-Cas9, 1:1000, red)and the DNA stained blue with DAPI. The yellow label is the overlapping of the Anti-Cas9 antibody and the Cas9-GFP construct.

Western blot of GFP-Cas9 transfected HEK293 cell lysate (+) showing specific immunolabeling of the ~53 kDa GFP-Cas9 fusion protein. Labeling is absent in the untransfected lysate (-).

PhosphoSolutions Anti-CRISPR Cas9 Antibody

From $160

Western blot of human Jurkat cell lysate. The blot was probed with mouse monoclonal anti-Crk II (C-terminal region) antibody at 1:250 (lane 1), 1:500 (lane 2), or 1:1000 (lane 3).

PhosphoSolutions Anti-Crk II (C-terminal region) Antibody

$285

Western blot of human K562 cells stimulated with pervanadate (1 mM) for 30 min. (lanes 1-4). The blot was treated with alkaline phosphatase to dephosphorylate Crk II (lanes 2 & 4), then the blot was probed with mouse monoclonal Crk II (C-terminal region) CM3321 (lanes 1 & 2) and Crk II (Tyr-221) phospho-specific CP4701 (lanes 3 & 4).

PhosphoSolutions Anti-Crk II (Tyr-221), Phosphospecific Antibody

$340

Western blot of human K562 cells stimulated with pervanadate (1 mM) for 30 min. (lanes 1-6). The blot was treated with akaline phosphatase to dephosphorylate Crk (lanes 2, 4 & 6), then the blot was probed with rabbit polyclonals CrkL (C-terminus) CP3081 (lanes 1 & 2), Crk (Tyr-251) phospho-specific CP3091 (lanes 3 & 4), and mouse monoclonal anti-Crk II (C-terminal region) (lanes 5 & 6).

PhosphoSolutions Anti-Crk II (Tyr-251), Phosphospecific Antibody

$340

PhosphoSolutions Anti-CrkL (C-terminus) Antibody

$285

PhosphoSolutions Anti-CrkL (Tyr-207), Phosphospecific Antibody

$340

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