Our Anti-Alpha-2C adrenergic receptor mouse monoclonal primary antibody from NeuroMab is produced in-house from hybridoma clone N330A/80. It is KO validated, detects human and mouse Alpha-2C adrenergic receptor, and is purified by Protein A chromatography. It is great for use in IHC, ICC, WB.
Transfected cell immunoblot: extracts of COS cells transiently transfected with HA-tagged Alpha-2C and Alpha-2A adrenergic receptor or untagged Kv2.1 plasmid and probed with N330A/80 TC supe.
α-2C adrenergic receptor or α-2 Adrenergic Receptor Subtype C4 is encoded by the gene ADRA2C. It belongs to the Adrenergic receptor subfamily of G-protein coupled receptors. α-2C adrenergic receptors mediate catecholamine-induced inhibition of adenylate cyclase by the action of G proteins. α-2C adrenergic is involved in the regulation of neurotransmitter release from sympathetic nerves and adrenergic neurons. α-2C adrenergic receptors are expressed in brain, kidney and heart. Diseases associated with ADRA2C include Raynaud Phenomenon.
Purified by Protein A chromatography
1 mg/mL
Monoclonal
N330A/80
IgG1
ICC, IHC, WB
Mouse
ADRA2C ADRA2L2 ADRA2RL2
50 kDa and larger (possibly due to dimerization)
Synthetic peptide amino acids 442-462 (QDFRRSFKHILFRRRRRGFRQ, cytoplasmic C-terminus) of human Alpha-2C adrenergic receptor (accession number P18825)
Human, Mouse
AB_2315797
Aliquot and store at ≤ -20°C for long term storage. For short term storage, store at 2-8°C. For maximum recovery of product, centrifuge the vial prior to removing the cap.
Liquid
Produced by in vitro bioreactor culture of hybridoma line followed by Protein A affinity chromatography. Purified mAbs are >90% specific antibody.
10 mM Tris, 50 mM Sodium Chloride, 0.065% Sodium Azide pH 7.125
Unconjugated
Does not cross-react with other adrenergic receptors (based on KO validation results)
Each new lot of antibody is quality control tested on cells overexpressing target protein and confirmed to give the expected staining pattern.
These antibodies are to be used as research laboratory reagents and are not for use as diagnostic or therapeutic reagents in humans.
