Anti-Ankyrin-B Antibody (N105/17)

Our Anti-Ankyrin-B mouse monoclonal primary antibody from NeuroMab is produced in-house from hybridoma clone N105/17. It detects human, mouse, and rat Ankyrin-B, and is purified by Protein A chromatography. It is great for use in IHC, ICC, WB.



SKU: 75-145

Volume: 100 µL
1-2 business days
Price:
Sale price$385.00

Product Details

Ankyrin-B
Ankyrin-2 or -Ankyrin-B is encoded by the gene ANK2. Ankyrin B is a scaffold protein that functions to link proteins that mediate the attachment of integral membrane proteins to the spectrin-actin based membrane cytoskeleton. In this manner, ankyrin family proteins play key roles in various processes including cell motility, activation, proliferation, contact and the maintenance of specialized membrane domains. Ankyrin B is expressed in brain and muscle. Mutations in this gene cause long QT syndrome 4 and cardiac arrhythmia syndrome.
Purified by Protein A chromatography
1 mg/mL
Monoclonal
N105/17
IgG2a
ICC, IHC, IP, WB
Mouse
ANK2 ANKB
<200 kDa
Fusion protein amino acids 203-496 of human Ankyrin-B (accession number Q01484) produced recombinantly in E. Coli
Guinea Pig, Human, Mouse, Rat
AB_2057705
Aliquot and store at ≤ -20°C for long term storage. For short term storage, store at 2-8°C. For maximum recovery of product, centrifuge the vial prior to removing the cap.
Liquid
Produced by in vitro bioreactor culture of hybridoma line followed by Protein A affinity chromatography. Purified mAbs are >90% specific antibody.
10 mM Tris, 50 mM Sodium Chloride, 0.065% Sodium Azide pH 7.71
WB: 1:1000
IHC: 1:200
ICC: 1:200
Unconjugated
No cross-reactivity reported
Each new lot of antibody is quality control tested by western blot on rat whole brain lysate and confirmed to stain the expected molecular weight band.
These antibodies are to be used as research laboratory reagents and are not for use as diagnostic or therapeutic reagents in humans.
United States
24 months from date of receipt
Shipped on ice packs
Ankyrin-2 (ANK-2) (Ankyrin-B) (Brain ankyrin) (Non-erythroid ankyrin)

Product Specific References for Applications and Species

Immunocytochemistry: Mouse
PMID Dilution Publication
336896791:10Chang, K.J., et al. 2021. TDP-43 maximizes nerve conduction velocity by repressing a cryptic exon for paranodal junction assembly in Schwann cells. . Elife, e64456.
25362473not listedHo, T.S., et al. 2015. A hierarchy of ankyrin-spectrin complexes clusters sodium channels at nodes of Ranvier.. Nature Neuroscience, 1664-72.
Immunocytochemistry: Rat
PMID Dilution Publication
319348591:500Yang, H.Q., et al. 2020. Ankyrin-G mediates targeting of both Na + and K ATP channels to the rat cardiac intercalated disc. Elife, e52373.
22632975not listedGaliano, M.R., et al. 2012. A distal axonal cytoskeleton forms an intra-axonal boundary that controls axon initial segment assembly.. Cell, 1125-1139.
21518878not listedSusuki, K., et al. 2011. Schwann cell spectrins modulate peripheral nerve myelination.. PNAS: USA, 8009-8014.
Immunohistochemistry: Guinea Pig
PMID Dilution Publication
213902231:100Huff, T.B., et al. 2011. Real-time CARS imaging reveals a calpain-dependent pathway for paranodal myelin retraction during high-frequency stimulation.. PLoS One, e17176.
Immunohistochemistry: Mouse
PMID Dilution Publication
367105001:300Cunningham, M.E., et al. 2023. Axolemmal Nanoruptures Arising from Paranodal Membrane Injury Induce Secondary Axon Degeneration in Murine Guillain-Barré Syndrome. Journal of the Peripheral Nervous System, 17-31.
356711051:300McGonigal, R., et al. 2022. Schwann Cell Nodal Membrane Disruption Triggers Bystander Axonal Degeneration in a Guillain-Barré Syndrome Mouse Model. The Journal of Clinical Investigation, e158524.
28128343not listedMooney, C.M., et al. 2017. RNA sequencing of synaptic and cytoplasmic Upf1-bound transcripts supports contribution of nonsense-mediated decay to epileptogenesis. Scientific Reports, 41517.
25362471not listedChang, K.J., et al. 2014. Glial ankyrins facilitate paranodal axoglial junction assembly.. Nature Neuroscience, 1673-81.
23728480not listedLe Bras, B., et al. 2014. In vivo assembly of the axon initial segment in motor neurons.. Brian Structure and Function, 1433-1450.
22131424not listedKaphzan, H., et al. 2011. Alterations in intrinsic membrane properties and the axon initial segment in a mouse model of Angelman syndrome.. Journal of Neuroscience, 17637-17648.
Immunohistochemistry: Rat
PMID Dilution Publication
319348591:500Yang, H.Q., et al. 2020. Ankyrin-G mediates targeting of both Na + and K ATP channels to the rat cardiac intercalated disc. Elife, e52373.
29853631not listedSusuki, K., et al. 2018. Glial βII spectrin contributes to paranode formation and maintenance. Journal of Neuroscience, 6063-6075.
217343021:2000Lysakowski, A., et al. 2011. Molecular microdomains in a sensory terminal, the vestibular calyx ending.. Journal of Neuroscience, 10101-10114.
Immunoprecipitation: Rat
PMID Dilution Publication
25362471not listedChang, K.J., et al. 2014. Glial ankyrins facilitate paranodal axoglial junction assembly.. Nature Neuroscience, 1673-81.
STORM: Rat
PMID Dilution Publication
319348591:50Yang, H.Q., et al. 2020. Ankyrin-G mediates targeting of both Na + and K ATP channels to the rat cardiac intercalated disc. Elife, e52373.
Western Blot: Mouse
PMID Dilution Publication
310421471:100Wang, G., et al. 2019. Structural plasticity of actin-spectrin membrane skeleton and functional role of actin and spectrin in axon degeneration. Elife, e38730.
247955611:1000Lugo, J.N., et al. 2014. Deletion of PTEN produces autism-like behavioral deficits and alterations in synaptic proteins.. Frontiers in Molecular Neuroscience, 27.
24217619not listedZhang, C., et al. 2013. Membrane domain organization of myelinated axons requires βII spectrin.. The Journal of Biology, 437-443.
Western Blot: Rat
PMID Dilution Publication
319348591:2000Yang, H.Q., et al. 2020. Ankyrin-G mediates targeting of both Na + and K ATP channels to the rat cardiac intercalated disc. Elife, e52373.

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