Our Anti-ASIC1 mouse monoclonal primary antibody from NeuroMab is produced in-house from hybridoma clone N271/44. It is KO validated, detects mouse and rat ASIC1, and is purified by Protein A chromatography. It is great for use in IHC, ICC, WB.
Transfected cell immunoblot: extracts of COS cells transiently transfected with GFP-tagged ASIC1, ASIC2 or untagged Kv2.1 plasmid and probed with N271/44 (left) or N86/8 (right) TC supe.
Acid sensing ion channel 1, Neuronal amiloride-sensitive cation channel 2 or ASIC1 is encoded by the gene ASIC1 and is a member of the Acid sensing ion channel (ASIC) family. ASIC1 acts as an acid sensing proton gated sodium channel and is thought to play a role in learning, pain transduction, memory development, and fear conditioning. ASIC1 is widely expressed in the central and peripheral nervous system. Diseases associated with ASIC1 include spatial learning and memory capability problems and Frozen Shoulder.
Purified by Protein A chromatography
1 mg/mL
Monoclonal
N271/44
IgG1
ICC, IHC, IP, WB
Mouse
Asic1 Accn2 Asic Bnac2
60 kDa
Fusion protein amino acids 460-526 (cytoplasmic C-terminus) of mouse ASIC1 (accession number Q6NXK8) produced recombinantly in E. Coli
Human, Mouse, Rat
AB_11030370
Aliquot and store at ≤ -20°C for long term storage. For short term storage, store at 2-8°C. For maximum recovery of product, centrifuge the vial prior to removing the cap.
Liquid
Produced by in vitro bioreactor culture of hybridoma line followed by Protein A affinity chromatography. Purified mAbs are >90% specific antibody.
10 mM Tris, 50 mM Sodium Chloride, 0.065% Sodium Azide pH 7.125
WB: 1:1000
IHC: 1:1000
IP: 1.5 µL
Unconjugated
Does not cross-react with ASIC2
Each new lot of antibody is quality control tested by western blot on rat whole brain lysate and confirmed to stain the expected molecular weight band.
These antibodies are to be used as research laboratory reagents and are not for use as diagnostic or therapeutic reagents in humans.
United States
24 months from date of receipt
Shipped on ice packs
Acid-sensing ion channel 1 (ASIC1) (Acid-sensing ion channel) (Amiloride-sensitive cation channel 2, neuronal) (Brain sodium channel 2) (BNaC2)
Yang, J., et al. 2020. Acid-Sensing Ion Channels Contribute to Type III Adenylyl Cyclase-Independent Acid Sensing of Mouse Olfactory Sensory Neurons. Molecular Neurobiology, 3042-3056.
Leisle, L., et al. 2021. Dynorphin Neuropeptides Decrease Apparent Proton Affinity of ASIC1a by Occluding the Acidic Pocket. Journal of Medicinal Chemistry, 13299-13311.
Leisle, L., et al. 2021. Dynorphin Neuropeptides Decrease Apparent Proton Affinity of ASIC1a by Occluding the Acidic Pocket. Journal of Medicinal Chemistry, 13299-13311.
Rook, M.L., et al. 2021. Mutation of a conserved glutamine residue does not abolish desensitization of acid-sensing ion channel 1. The Journal of General Physiology, .
Neuhof, A., et al. 2021. Large Acid-Evoked Currents, Mediated by ASIC1a, Accompany Differentiation in Human Dopaminergic Neurons. Frontiers in Cellular Neuroscience, 668008.
Farrag, M., et al. 2017. Endomorphins potentiate acid-sensing ion channel currents and enhance the lactic acid-mediated increase in arterial blood pressure: effects amplified in hindlimb ischaemia.. Journal of Physiology, 7167-7183.
Kim, J.S., et al. 2019. Effect of knockout of the ASIC3 on cardiovascular reflexes arising from hindlimb muscle in decerebrated rats. American Journal of Physiology. Regulatory, Integrative, and Comparative Physiology, R641-R648.
Diochot, S., et al. 2016. Analgesic effects of mambalgin peptide inhibitors of acid-sensing ion channels in inflammatory and neuropathic pain. Pain, 552-559.
